We narrowed to 9,236 results for: mel
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Plasmid#166133PurposeThis plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistanceDepositorInserthU6-gRNA and hH1-gRNA targeting cldn1 gene
UseCRISPR and LentiviralTagsiRFP670ExpressionMammalianAvailable SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro
Plasmid#166134PurposeThis plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistanceDepositorInserthU6-gRNA and hH1-gRNA targeting firefly luciferase gene
UseCRISPR and LentiviralTagsiRFP670ExpressionMammalianAvailable SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF385A
Plasmid#101615PurposeDonor Vector containing ZNF385A transcription factor, part of the Human TFome CollectionDepositorInsertZNF385A (ZNF385A Human)
UseGateway shuttling vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-FOXD4L3
Plasmid#101491PurposeDonor Vector containing FOXD4L3 transcription factor, part of the Human TFome CollectionDepositorInsertFOXD4L3 (FOXD4L3 Human)
UseGateway shuttling vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-TDP-43
Plasmid#162608PurposeAllows for transcription of TDP-43 mRNA for injection into zebrafish embryos for BiFC assaysDepositorInsertTARDBP (TARDBP Human)
ExpressionMammalianAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF197
Plasmid#101547PurposeDonor Vector containing ZNF197 transcription factor, part of the Human TFome CollectionDepositorInsertZNF197 (ZNF197 Human)
UseGateway shuttling vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF660
Plasmid#88673PurposeDonor Vector containing ZNF660 transcription factor, part of the Human TFome CollectionDepositorInsertZNF660 (ZNF660 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF302
Plasmid#88279PurposeDonor Vector containing ZNF302 transcription factor, part of the Human TFome CollectionDepositorInsertZNF302 (ZNF302 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF322
Plasmid#88327PurposeDonor Vector containing ZNF322 transcription factor, part of the Human TFome CollectionDepositorInsertZNF322 (ZNF322A Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG442
Plasmid#165616PurposeVector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)DepositorInsertTEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
UseCRISPRTags3x HA, SV40 NLS, Zif268, and c-Myc-like NLSExpressionYeastMutationCorrected homology relative to wild type SpCas9 (…PromoterTEF1Available SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK-Ptrf-mKate2
Plasmid#128765PurposeFluorescent mKate reporter for PtrfDepositorAvailable SinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-MCS2-EF1-Puro-RGS7-R44C+V56C
Plasmid#113730PurposeExpress double mutant RGS7- R44C+V65C in mamalian cellsDepositorInsertRGS7 (RGS7 Human)
UseLentiviralTagsFlagExpressionMammalianMutationR44C and V56CPromoterCMVAvailable SinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSSO2462
Plasmid#97064PurposeExpressing Sulfolobus solfataricus orf SSO2462 proteinDepositorInsertS. solfataricus orf SSO2462
TagsN-terminal TEV protease cleavable 6xHis tagMutationnonePromoterT7Available SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSSO2452
Plasmid#97059PurposeExpressing Sulfolobus solfataricus orf SSO2452 proteinDepositorInsertS. solfataricus orf SSO2452
TagsN-terminal TEV protease cleavable 6xHis tagMutationnonePromoterT7Available SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPSVORF126
Plasmid#97021PurposeExpressing Pyrobaculum spherical virus orf126 proteinDepositorInsertPSV orf 126
TagsN-ter TEV protease cleavable 6HIsMutationnonePromoterT7Available SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA5006
Plasmid#97016PurposeExpressing Pseudomonas aeruginosa orf PA5006 proteinDepositorInsertP aeruginosa orf PA5006
TagsN-ter TEV protease cleavable 6HIsMutationnonePromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEHISSIRV119
Plasmid#97054PurposeExpressing Sulfolobus islandicus rudivirus (SIRV) orf 119 proteinDepositorInsertSIRV orf 119
TagsN-terminal TEV protease cleavable 6xHis tagMutationnonePromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEHISSIRV55
Plasmid#97055PurposeExpressing Sulfolobus islandicus rudivirus (SIRV) orf 55 proteinDepositorInsertSIRV orf 55
TagsN-terminal TEV protease cleavable 6xHis tagMutationnonePromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA0856
Plasmid#97014PurposeExpressing Pseudomonas aeruginosa orf PA0856 proteinDepositorInsertP aeruginosa orf PA0856
TagsN-ter TEV cleavable 6HIsMutationN-terminal deletion (AA 1-34)PromoterT7Available SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA4631
Plasmid#97017PurposeExpressing Pseudomonas aeruginosa orf PA4631 proteinDepositorInsertNucleoside-diphosphate-sugar epimerase
TagsN-ter TEV protease cleavable 6HIsMutationnonePromoterT7Available SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only