We narrowed to 9,186 results for: mel

1. px552-U6-gRNA1-U6-gRNA2-CMV-eGFP

   Plasmid

   #211760

   Purpose

   Paired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

   Depositor

   Glenn Yiu

   Article

   Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

   Insert

   gRNA1 and gRNA2 targeting VEGF-A (VEGFA Mouse, M. mulatta (rhesus macaque), Human)
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Caspase-3 (C163A)-pcw107-V5

   Plasmid

   #64633

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   CASP3 (transcript variant beta) (CASP3 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   C163A

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-EF1α1.1-ChrimsonR-tdTomato

   Plasmid

   #122064

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the EF1α promoter (1.1kb short version).tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato (codon diversified version)

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   EF1a（1.1kb short version)

   Available Since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SmoM2 (W535L)-pcw107-V5

   Plasmid

   #64628

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   SMO (SMO Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   W535L

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CaMKII-ChrimsonR-tdTomato

   Plasmid

   #99231

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   CaMKII

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-Syn-Chronos-tdTomato

   Plasmid

   #62726

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the Syn promoter. Using bGHpA signal. tdTomato has codons varied between the first and second tandem repeats to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV8

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   human synapsin promoter

   Available Since

   April 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. beta-catenin (S33Y)-pcw107

   Plasmid

   #64615

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Lentiviral

   Mutation

   S33Y

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPaint-mScarlet-PuroR [M1G]

   Plasmid

   #171809

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-T2A-PuroR
   

   Use

   Gene tagging

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BCL-XL-pcw107-V5

   Plasmid

   #64631

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   BCL2L1 (transcript variant 1) (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pLKO.1.sh.beta-catenin.2279

    Plasmid

    #19762

    Depositor

    William Hahn

    Article

    Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

    Insert

    small hairpin RNA against beta-catenin (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-Syn-FLEX-rc [ChrimsonR-GFP]

    Plasmid

    #84480

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    human synapsin promoter

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR221-GATA6

    Plasmid

    #101418

    Purpose

    Donor Vector containing GATA6 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    GATA6 (GATA6 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CMV-SpCas9D10A nickase

    Plasmid

    #216737

    Purpose

    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMVd1

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pN1 Lck-GCaMP5G

    Plasmid

    #34924

    Purpose

    Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane

    Depositor

    Baljit Khakh

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    Lck-GCaMP5G
    

    Expression

    Mammalian

    Mutation

    Plasmid contains N-terminal 26 amino acid membran…

    Promoter

    CMV

    Available Since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS2plus-TDP-43 M337V

    Plasmid

    #162609

    Purpose

    Allows for transcription of mutant TDP-43-M337V mRNA for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    TARDBP (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    M337V

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRISPaint-mScarlet-BlastR [M1G]

    Plasmid

    #171814

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-T2A-BlastR
    

    Use

    Gene tagging

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX330-TP53-1

    Plasmid

    #121917

    Purpose

    Encodes sgRNA targeting exon 4 of TP53

    Depositor

    William Hahn

    Article

    Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330-TP53-2

    Plasmid

    #121918

    Purpose

    Encodes sgRNA targeting exon 9 of TP53

    Depositor

    William Hahn

    Article

    Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SmoM2 (W535L)-pcw107

    Plasmid

    #64627

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    SMO (SMO Human)
    

    Use

    Lentiviral

    Mutation

    W535L

    Promoter

    PGK

    Available Since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

20. myr-FLAG-AKT1-pcw107

    Plasmid

    #64606

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and myristoylated

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only