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Expression_vector_NT_1

Plasmid

#158023

Purpose

Expression vector for the de novo designed protein Expression_vector_NT_1

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein NT_1

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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Expression_vector_NT_8

Plasmid

#158024

Purpose

Expression vector for the de novo designed protein Expression_vector_NT_8

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein NT_8

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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Expression_vector_NT_9

Plasmid

#158025

Purpose

Expression vector for the de novo designed protein Expression_vector_NT_9

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein NT_9

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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Expression_vector_NT_10

Plasmid

#158026

Purpose

Expression vector for the de novo designed protein Expression_vector_NT_10

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein NT_10

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pBS_12x-601_MA1

Plasmid

#114358

Purpose

12x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Article

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

12X601-MA1

Use

Unspecified

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLdCN2

Plasmid

#125186

Purpose

Expresses Streptococcus pyogenes Cas9 (SpCas9) and two gRNAs in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al Methods Mol Biol. 2020;2116:199-224. doi

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-RapGAP

Plasmid

#118324

Purpose

Expression of RapGAP

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

RapGAP

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-RR-zrif1_exon8

Plasmid

#194434

Purpose

Right (RR) TALEN for mutating zebrafish rif1 gene

Depositor

Christopher Sansam

Article

Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

Insert

zrif1_ex8_RIGHT (rif1 Zebrafish)

Use

TALEN

Promoter

CMV IE94

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-DD-zrif1_exon8

Plasmid

#194435

Purpose

Left (DD) TALEN for mutating zebrafish rif1 gene

Depositor

Christopher Sansam

Article

Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

Insert

zrif1_ex8_LEFT (rif1 Zebrafish)

Use

TALEN

Promoter

CMV IE94

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYTK-DN2

Plasmid

#180283

Purpose

Spacer cassette (TU3)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConL2 - Spacer - ConRE

Expression

Bacterial

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSB1K3_M13ps

Plasmid

#190434

Purpose

M13 packaging signal cloned into the pSB1K3 vector.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

M13 packaging signal

Use

Synthetic Biology

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSH88

Plasmid

#127703

Purpose

Pacr-2::gfp1-10

Depositor

David M. Miller

Article

He et al Genetics. 2019 Apr 5. pii: genetics.119.

Insert

GFP1-10

Expression

Worm

Promoter

acr-2

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pYTK-DN3

Plasmid

#180284

Purpose

Spacer cassette (TU2)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConL1 - Spacer - ConR2

Expression

Bacterial

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJL030

Plasmid

#53533

Purpose

supplies EtpC required for optimal EtpA expression on compatible plasmid

Depositor

James Fleckenstein

Article

Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

Insert

etpC

Use

Pacyc184

Expression

Bacterial

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pWS3805

Plasmid

#185746

Purpose

Sub-array spacer 1/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 1/4

Use

CRISPR

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pWS3806

Plasmid

#185747

Purpose

Sub-array spacer 2/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 2/4

Use

CRISPR

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMA3160

Plasmid

#35043

Depositor

Mikhail Alexeyev

Article

Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

Tet

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCis_2+7+8+5

Plasmid

#60589

Purpose

a 4-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_2+7+8+5

Expression

Bacterial

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pWS3808

Plasmid

#185749

Purpose

Sub-array spacer 4/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 4/4

Use

CRISPR

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBS_24x-601_MA1+MA2

Plasmid

#114361

Purpose

24x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Article

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

24X601-MA1_2

Use

Unspecified

Expression

Mammalian

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge