We narrowed to 7,293 results for: Ank

1. qTAG-C-Solo-mStayGold-LAMP1

   Plasmid

   #227322

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-N-Puro-mStayGold-ACTB

   Plasmid

   #227320

   Purpose

   Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-N-Solo-mStayGold-ACTB

   Plasmid

   #227326

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a mStayGold Tag (ACTB Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-N-Solo-mScarlet-LMNB1

   Plasmid

   #207771

   Purpose

   Donor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a mScarlet Tag (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. qTAG-N-Puro-mStayGold-LMNB1

   Plasmid

   #227329

   Purpose

   Donor template for Puro-2A-mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Puro-2A-mStayGold (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLP-PIGA (pLP140)

   Plasmid

   #168461

   Purpose

   Big-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.

   Depositor

   Jef Boeke, Matthew Maurano

   Article

   Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

   Type

   Empty backbone

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Blast-dTAG-LMNB1

   Plasmid

   #207776

   Purpose

   Donor template for Blast-2A-dTAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-dTAG Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. FLAG-LoxP-PURO-LoxP-SNAP-TERT

   Plasmid

   #71390

   Purpose

   Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.

   Depositor

   Thomas Cech

   Article

   Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

   Insert

   TERT (TERT Human)
   

   Use

   Hr donor

   Tags

   FLAG-SNAP

   Promoter

   Endogenous TERT promotor

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-N-Blast-mScarlet-ACTB

   Plasmid

   #207753

   Purpose

   Donor template for Blast-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Blast-mScarlet Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Dec. 1, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCAG-mScarlet-Giantin

    Plasmid

    #196872

    Purpose

    Expresion of Giantin-fused to mScarlet. Used as Golgi marker

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mScarlet-Giantin (GOLGB1 Synthetic, Human)
    

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-N-Solo-mStayGold-CEP135

    Plasmid

    #227287

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the CEP135 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CEP135 (Addgene #227286)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CEP135 Homology Arms flanking a mStayGold Tag (CEP135 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-N-Puro-moxGFP-GOLGA2

    Plasmid

    #207792

    Purpose

    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    GOLGA2 Homology Arms flanking a Puro-moxGFP Cassette (GOLGA2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLVX-BSD-tet-mEGFP-MIB1

    Plasmid

    #140241

    Purpose

    Lentiviral vector expressing mEGFP-tagged MIB1 upon tetracycline treatment

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    Tet-inducible mEGFP-MIB1 (MIB1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

    Plasmid

    #198240

    Purpose

    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites

    Depositor

    Didier Stainier

    Article

    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

    Inserts

    Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)
    

    Use

    Zebrafish transgenesis

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-N-Solo-mStayGold-GOLGA2

    Plasmid

    #227325

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the GOLGA2 locus. For Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 (Addgene #207791)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    GOLGA2 Homology Arms flanking a mStayGold Tag (GOLGA2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TVBB C-term-mScarlet-Blast

    Plasmid

    #169221

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Blast at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mScarlet-P2A-Blast
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PL-5LTR-RGR(DMD​#​1​)-AmCyan-A

    Plasmid

    #138482

    Purpose

    Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    Ribozyme-flanked gRNA and AmCyan
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-N-Blast-mNeon-ACTB

    Plasmid

    #207751

    Purpose

    Donor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. qTAG-C-moxGFP-Puro-LAMP1

    Plasmid

    #207788

    Purpose

    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LAMP1 Homology Arms flanking a moxGFP-Puro Cassette (LAMP1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Dec. 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. TVBB C-term-mScarlet-Puro

    Plasmid

    #169220

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mScarlet-P2A-Puro
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge