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pBS-EF1afull-H2B-mCherry-P2A-Fluc

Plasmid

#207253

Purpose

Lentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoter

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

H2B-mCherry-P2A-Fluc

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha_full

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMW37: pRS426-R2Bm RNA

Plasmid

#209291

Purpose

Contains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequences

Depositor

Feng Zhang

Article

Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

Insert

R2Bm 5'UTR and 3'UTR

Expression

Bacterial and Yeast

Promoter

GAL1 GAPDH

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP FLAG

Plasmid

#59756

Purpose

encodes multiple copies of an epitope tag FLAG

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS2

Plasmid

#196978

Purpose

Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS2 tag

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP HA

Plasmid

#59759

Purpose

encodes multiple copies of an epitope tag HA

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _HA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP V5

Plasmid

#59758

Purpose

encodes multiple copies of an epitope tag V5

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _V5

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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Cys-60nmSAH-SPYcatcher

Plasmid

#214840

Purpose

This plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

Cys-60nmSAH-SPYcatcher

Tags

His-TEV-SUMO-

Expression

Bacterial

Promoter

T5

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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Cys-3HB-SPYcatcher

Plasmid

#214838

Purpose

This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

Cys-3HB-SPYcatcher

Tags

His-TEV-SUMO-

Expression

Bacterial

Promoter

T5

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET-45b-mCherry

Plasmid

#145279

Purpose

Bacterial Expression of 6xHis mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

mCherry

Expression

Bacterial

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-ABEmax-BlastR

Plasmid

#152989

Purpose

A-to-G base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

ABE7.10-nSpCas9

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS1

Plasmid

#196977

Purpose

Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS1 tag

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-truncated

Plasmid

#198286

Purpose

Contains a tail fiber with a truncated binding domain (lacking aa403-476 in Pvc13); produces nontargeting PVCs

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-truncated

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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dCas9-CRY2Clust

Plasmid

#200969

Purpose

induction of phase-separated condensate by CRY2Clust

Depositor

Jian Xu

Article

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

CRY2Clust

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Beclin1

Plasmid

#21150

Depositor

Junying Yuan

Article

Shibata et al J Biol Chem. 2006 May 19. 281(20):14474-

Insert

PcDNA3-Beclin1 (BECN1 Human)

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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alpha7nACHR_Nacho_mCherry

Plasmid

#182939

Purpose

alpha7 nACHR expression in mammalian cell lines

Depositor

Robert Brenner

Article

Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

Insert

NACH7 (CHRNA7 Human)

Use

Synthesized

Expression

Mammalian

Promoter

EF1alpha

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pJMK077: CaMKIIa QuasAr2-TS-GCaMP6f

Plasmid

#72304

Purpose

Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

Depositor

Adam Cohen

Article

Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

Insert

CaViar

Use

Lentiviral

Tags

TS

Expression

Mammalian

Promoter

CaMKIIa

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMVV099

Plasmid

#215305

Purpose

Expresses sfGFP under induction with the Cuminic Acid Marionette Cassette (CymRAM + PCymRC-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PCymRC

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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AAV2_HLF-ZE

Plasmid

#175034

Purpose

HLF targeting construct introducing an ires ZsG P2A tEGFR cassette downstream of the human HLF stop codon

Depositor

Guy Sauvageau

Article

Lehnertz et al Blood. 2021 Sep 9. pii: S0006-4971(21)01

Insert

HLF ires ZsGreen P2A tEGFR

Use

AAV

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-BCL6-IRES-GFP

Plasmid

#31391

Depositor

Hilda Ye

Article

Yu et al Blood. 2005 Feb 15. 105(4):1777-84.

Insert

B-cell lymphoma 6 (BCL6 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP Myc

Plasmid

#59757

Purpose

encodes multiple copies of an epitope tag Myc

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _Myc

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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