We narrowed to 166,390 results for: addgene

1. GFP-OMP25

   Plasmid

   #141150

   Purpose

   Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   OMP25 (Synj2bp Rat)
   

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   OMP25 c terminus from addgene plasmid #69598

   Promoter

   CMV

   Available Since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p300dAIL KAT LIC 1M

   Plasmid

   #233588

   Purpose

   Bacterial expression of p300 KAT enzyme with truncated autoinhibitory loop

   Depositor

   Scott Showalter

   Article

   Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

   Insert

   p300dAIL KAT (EP300 Human)
   

   Tags

   6xHis MBP

   Expression

   Bacterial

   Mutation

   deleted amino acids 1523-1554

   Promoter

   T7

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenSyn2-EGFP-­OMP25C

   Plasmid

   #71427

   Purpose

   Lentiviral expression of EGFP-OMP25C. For visualization of neuronal mitochondria.

   Depositor

   Henri Huttunen

   Article

   Kysenius et al PLoS One. 2014 Sep 5;9(9):e107129. doi:

   Insert

   OMP25 (172-­‐206) (Synj2bp Rat)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 172-206 only

   Promoter

   synapsin

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-hsyn-GRAB_HA1mut

   Plasmid

   #190475

   Purpose

   Express the histamine-insensitive control sensor GRAB_HA1mut in neurons

   Depositor

   Yulong Li

   Article

   Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

   Insert

   Histamine-insensitive sensor GRAB_HA1mut (control sensor)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA-CasRx_SD1-pSV40-TagBFP

   Plasmid

   #221004

   Purpose

   Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

   Depositor

   Benjamin Blencowe, Mikko Taipale

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   CasRx 30nt processed direct repeat with SD1 mutation
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   DR1 A>T

   Promoter

   U6

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJRH-1363 LV EF1a-CD19 IRES-EGFP

   Plasmid

   #201919

   Purpose

   2nd generation lentiviral transfer plasmid for engineering cells to express human CD19

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   CD19 (CD19 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-AAVS1-Ef1a-Puro-moxGFP

   Plasmid

   #227273

   Purpose

   AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMVtight-UPRT-T2A-RFP-IRES-CD

   Plasmid

   #126677

   Purpose

   Doxycycline inducible expression of UPRT and CD along with reporter RFP

   Depositor

   Joan Massague

   Article

   Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

   Insert

   UPRT-T2A-RFP-IRES-CD (FCY1 Budding Yeast, Toxoplasma gondii)
   

   Promoter

   tight TRE

   Available Since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCasTet-λ

   Plasmid

   #128318

   Purpose

   Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

   Depositor

   Christopher Johnston

   Article

   Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

   Insert

   TetR and pTetR/TetO
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Mutation

   Constitutive expression of cas9 and anhydro-tetra…

   Promoter

   tet promoter

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. QPM-sgR (pTaU3)

    Plasmid

    #140448

    Purpose

    For sgRNA expression in monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

    Insert

    TaU3-sgRNA
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    TaU3

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. HIS-HA-CMYC P53

    Plasmid

    #233738

    Purpose

    expresses HIS-HA-CMYC triple tagged P53 for pulldown assay

    Depositor

    Albert Keung

    Article

    Han et al Methods. 2025 Mar;235:92-99. doi: 10.101

    Insert

    HIS-HA-CMYC-p53 (TP53 Human)
    

    Tags

    HIS-HA-CMYC

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid

    #227274

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-EF1-FHC-POLQ

    Plasmid

    #64875

    Purpose

    lentiviral expression of human POLQ

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. plRL117

    Plasmid

    #163635

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet-On

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-GfaABC1D-GRAB_ATP1.0

    Plasmid

    #167579

    Purpose

    Express the ATP sensor GRAB_ATP1.0 in astrocytes

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP sensor GRAB_ATP1.0 (P2RY1 Human)
    

    Use

    AAV

    Promoter

    GfaABC1D

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. sUPRa

    Plasmid

    #223242

    Purpose

    sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels

    Depositor

    Colin Akerman

    Article

    Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10

    Insert

    sUPRa
    

    Use

    AAV

    Promoter

    Custom

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGM

    Plasmid

    #71250

    Purpose

    luciferase reporter with human GM-CSF promoter -627 to + 28

    Depositor

    Peter Cockerill

    Article

    Cockerill et al Proc Natl Acad Sci U S A. 1999 Dec 21;96

    Insert

    GM-CSF promoter -627 to +28 fragment (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    GM-CSF

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. TMPRSS3_pCSdest

    Plasmid

    #53839

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    TMPRSS3 (TMPRSS3 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pLenti_PEmax-2A-MLH1dn-2A-BSD

    Plasmid

    #234072

    Purpose

    For lentiviral expression of PEmax with MLH1dn and blasticidin resistance

    Depositor

    Greg Findlay

    Article

    Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

    Insert

    PEmax-2A-MLH1dn
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF-1α core promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid

    #201917

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Expression

    Mammalian

    Promoter

    CAG and Human U6

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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