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pHDR2_DAZL-T2A-mGreenLantern-PuroTK

Plasmid

#222917

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to DAZL.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_13

Plasmid

#194674

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_13 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_13

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-cpHaloTag141/145

Plasmid

#194660

Purpose

Protein production of circular permuted HaloTag (cpHaloTag141/145) in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

cpHaloTag141/145

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-cpHaloTag154/156

Plasmid

#194661

Purpose

Protein production of circular permuted HaloTag (cpHaloTag154/156) in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

cpHaloTag154/156

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-MAPTau-C-10

Plasmid

#55311

Purpose

Localization: Microtubules, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

MAPTau (MAPT Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBabe-CyPet-Rac1

Plasmid

#24534

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (RAC1 Human)

Use

Retroviral

Tags

CyPet

Expression

Mammalian

Available Since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only
BII-ChPtW-EGFP

Plasmid

#133400

Purpose

Piggybac vector for constitutive expression of EGFP

Depositor

Blair Madison

Article

Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

Insert

EGFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Cx26-7

Plasmid

#58007

Purpose

Localization: Gap Junctions, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Cx26 (Gjb2 Rat)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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33-G-X5-pEF1/V5-His

Plasmid

#111605

Purpose

Express human 33-G-X5 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 and GARP chimeria (NRROS Human)

Tags

Flag tag

Expression

Mammalian

Promoter

EF1a

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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PA-mCherry1-Vimentin-7

Plasmid

#57202

Purpose

Localization: Intermediate Filaments, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Article

York et al Nat Methods. 2011 Apr;8(4):327-33. doi:

Insert

Vimentin (VIM Human)

Tags

PA-mCherry1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_ORF3b_HA_BASU

Plasmid

#153992

Purpose

Express Orf3b gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

orf3b

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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mCardinal-Paxillin-22

Plasmid

#56171

Purpose

Localization: Focal Adhesions, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Paxillin

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pTriEX-Antenna-GDI Cdc42

Plasmid

#101681

Purpose

"Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al

Depositor

Klaus Hahn

Article

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

Cdc42

Tags

6xHis and FRET Antenna (mCerulean-cpVenus)

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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tdTomato-CENPB-N-22

Plasmid

#58081

Purpose

Localization: Nucleus/Centromeres, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Gao et al Cell. 2012 Dec 7;151(6):1370-85. doi: 10

Insert

CENPB (CENPB Human)

Tags

tdTomato

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
pEMS1988

Plasmid

#49119

Purpose

AAV plasmid with CAGGS promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-CAGGS-iCre

Use

AAV

Expression

Mammalian

Promoter

CAGGS

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pET-51b(+)-Caprola_09

Plasmid

#194670

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_09 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_09

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX ShcD-SH2

Plasmid

#46488

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

ShcD (SHC4 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 512-623

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SH2-B-SH2

Plasmid

#46479

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2-B (SH2B1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 515-639

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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mCerulean-LaminB1-10

Plasmid

#55380

Purpose

Localization: Nuclear Envelope, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

LaminB1 (LMNB1 Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
mCardinal-PMP-N-10

Plasmid

#56173

Purpose

Localization: Peroxisomes, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

PMP (PXMP2 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits