We narrowed to 11,390 results for: mpl
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Plasmid#146065PurposeInsect Expression of DmMe31B_1-267DepositorInsertDmMe31B_1-267 (me31B Fly)
ExpressionInsectAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B_268-459_C
Plasmid#146066PurposeInsect Expression of DmMe31B_268-459DepositorInsertDmMe31B_268-459 (me31B Fly)
ExpressionInsectAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-POLBHR-eGFP
Plasmid#176064PurposeHomology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1DepositorInsertEGFP-PolB (POLB Human)
TagsEGFPExpressionMammalianMutationpUC with endogenous SapI mutated out, PAM mutatio…PromoterN/AAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2308 - [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Plasmid#159852PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Gln93,Ala271 hygro
Plasmid#158638PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro
Plasmid#158639PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRepair-mTq2-CTNNB1
Plasmid#153433PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2DepositorInsertCTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsmTurquoise2Available SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FRT-TO-Nsp8-Nsp7_GC3opt
Plasmid#157700Purposemammalian expression of untagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoterDepositorInsertNsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
ExpressionMammalianMutationcodon optimized; gene expression was optimized by…Available SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEBTetDBl-CLIP-CPAP
Plasmid#136865PurposeMammalian expression of the centrosomal protein CENPJ N-terminally fused to CLIP-tagDepositorInsertCLIP-CENPJ (CENPJ Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-Cep290
Plasmid#136854PurposeMammalian expression of the centrosomal protein Cep290 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep290 (CEP290 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep290
Plasmid#136853PurposeMammalian expression of the centrosomal protein Cep290 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep290 (CEP290 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-CPAP
Plasmid#136816PurposeMammalian expression of the centrosomal protein CENPJ N-terminally fused to SNAP-tagDepositorInsertSNAP-CENPJ (CENPJ Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLB(N)CX AT-CH3-FLAG-HA WY
Plasmid#133725PurposeRetroviral vector with CMV promoter driving mutant form of acetyltransferase and CH3 domains fragment of human p300 (EP300) (WY1466-1467AS in AT domain).DepositorInsertEP300 (EP300 Human)
UseRetroviralTagsFlag and HAMutationpartial (not complete) p300 fragment, AT and CH3 …Available SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-Exoc7-Ex5&10
Plasmid#133303PurposeEGFP reporter sequence was removed from TLCV2 vector and two gRNAs targeting mouse Exoc7 gene were cloned into the modified TLCV2 vector.DepositorInsertExocyst complex component 7 (Exoc7 Mouse)
UseCRISPR and LentiviralExpressionMammalianPromotermU6 promoterAvailable SinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>Fab-srt-his
Plasmid#124810PurposeHDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.DepositorInsertrat IgG2a Hinge-G4S-Sortag-Histag
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterialPromoterpUCAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-NOS-IN133.3xFLAG-WPRE
Plasmid#127869PurposepAAV plasmid expressing an NOS-IN133.3xFLAG fusion protein under the hSyn promoterDepositorAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-GFP-Atg17(416) (I325D)
Plasmid#121412PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
ExpressionYeastMutationIsoleucine 325 to Aspartic acidPromoterCUP1 promoterAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-GFP-Atg17(416) (F317D)
Plasmid#121411PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
ExpressionYeastMutationPhenylalanine 317 to Aspartic acidPromoterCUP1 promoterAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-GFP-Atg17(416) (L105D)
Plasmid#121410PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
ExpressionYeastMutationLeucine 105 to Aspartic acidPromoterCUP1 promoterAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-Atg17(416) (L105D)
Plasmid#121405PurposeExpressing Atg17DepositorInsertCUP1p-ATG17 ORF-CYC1 terminator
ExpressionYeastMutationLeucine 105 to Aspartic acidPromoterCUP1 promoterAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_19kDa_EE
Plasmid#74951PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsGlu-Glu tag (EYMPME)ExpressionMammalianMutationdelta 1-127PromoterCMV, lentiviral LTRAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
DOT1L-CC3
Plasmid#111754PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
Tags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationContains coiled coil #3 (residues 613-661)PromoterT7Available SinceJune 14, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
DOT1L-CC1
Plasmid#111756PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
Tags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationcontains coiled coil #1 (residues 506-555)PromoterT7Available SinceJune 14, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
DOT1L-CC2
Plasmid#111755PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
Tags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationContains coiled coil #2 (residues 560-608)PromoterT7Available SinceJune 14, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCR3-vsv-INCENP d543-746
Plasmid#108473Purposeexpression of vsv-INCENP d543-746DepositorInsertINCENP d543-746 AA (INCENP Human)
TagsVSVExpressionMammalianMutationDelta Alpha helix, aa543-746 deletedPromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG251
Plasmid#80583Purposerecipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTADepositorInsertspnos:PAT-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
BsaI-ccdB_CmR-BsaI
UseCRISPRExpressionPlantMutationD10AAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-mCE(211-597)
Plasmid#82462PurposeExpression of myc epitope and C-terminal domain of murine mRNA Capping Enzyme in mammalian cellsDepositorAvailable SinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_25kDa_Myc
Plasmid#74954PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTags3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)ExpressionMammalianMutationdelta 1-77, M100A M128APromoterCMV, Lentiviral LTRAvailable SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT
Plasmid#61789PurposeLuciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutatedDepositorInsertmouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated
UseLuciferaseMutationboth miR-200c MRE-2 and MRE-3 mutatedAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
CFP(159-238) in pcDNAI/Amp
Plasmid#60896PurposeThis vector attaches CFP(159-238) to the N-terminus of a protein.DepositorInsertCFP(159-238)
ExpressionMammalianMutationAsn-164 to HisPromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only