We narrowed to 15,981 results for: LEA

1. pCMV_eA3A T31A-NG-nCas9

   Plasmid

   #163539

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A T31A-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. VP16-ER beta (long)

   Plasmid

   #11352

   Depositor

   Donald McDonnell

   Article

   Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

   Insert

   ER beta long (ESR2 Human)
   

   Expression

   Mammalian

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV2-CAG-mGreenLantern-KASH

   Plasmid

   #191094

   Purpose

   To express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Pantelis Tsoulfas, Murray Blackmore

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   mGreenLantern-KASH
   

   Use

   AAV and Synthetic Biology

   Tags

   mGreenLantern fused to the KASH domain of Nesprin…

   Expression

   Mammalian

   Mutation

   Optimizzation was done using the Human codon usage

   Promoter

   CMV enhancer and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pclbw-opa1(isoform 1)-myc

   Plasmid

   #62845

   Purpose

   Mammalian Expression of myc-tagged Opa1 isoform1

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   mmOPA1isoform1-myc (Opa1 Mouse)
   

   Use

   Retroviral

   Tags

   9xMyc

   Expression

   Mammalian

   Promoter

   cmv-bactin

   Available Since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pSynapsin_NLS_GFP_P2A_His6_GluA2

   Plasmid

   #170453

   Purpose

   Expresses histidine tagged GluA2 in neuronal cells

   Depositor

   Shigeki Watanabe

   Article

   Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

   Insert

   GluA2 (Gria2 Mouse)
   

   Use

   Lentiviral

   Tags

   Hexa-histidine

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVH-EF1a-GFP-P2A-KLF4

   Plasmid

   #130694

   Purpose

   Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   human KLF4 (KLF4 Human)
   

   Use

   Lentiviral

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKH3-TRIM28-C65A/C68A

   Plasmid

   #92199

   Purpose

   Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   Tif1b (TRIM28 Human, Synthetic)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Cysteines 65 and 68 to Alanines.

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-DroshaS300/302A

   Plasmid

   #62530

   Purpose

   Serines 300 and 302 of Drosha are mutated to alanines

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   human Drosha with serines 300 and 302 changed alanines (DROSHA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed serines 300 and 302 to alanines

   Available Since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pB-Tet-On-Arl13b-RhoA sensor

   Plasmid

   #189613

   Purpose

   Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   Arl13b
   

   Tags

   GFP and mScarlet

   Expression

   Mammalian

   Promoter

   Tet3G

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin

    Plasmid

    #133025

    Purpose

    Expresses EGFP-tagged tail anchor sequence of monoamine oxidase A

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Monoamine oxidase type A (MAOA Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-488

    Promoter

    Human elongation factor 1 alpha

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBK092

    Plasmid

    #183146

    Purpose

    pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expression

    Depositor

    Daan Swarts

    Article

    Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

    Insert

    CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-GFP-EphrinB3-Flag

    Plasmid

    #155012

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    EphrinB3 (EPHB3 Human)
    

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO_Ollas.S.V5_mCherry-NLS

    Plasmid

    #178211

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    Nuclear Localization Signal and Ollas.S.V5

    Promoter

    eF1a

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHis-LMW_FGF2

    Plasmid

    #157657

    Purpose

    Expresses FGF2 in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    changed Cystein 211,229 to serine, deleted amino …

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3xNLS-NLP-cMyc-cMyc LbaCas12a

    Plasmid

    #182125

    Purpose

    pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    3xNLS-NLP-cMyc-cMyc LbaCas12a
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. TVBB C-term-mNeongreen

    Plasmid

    #169227

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mNeongreen at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mNeongreen
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-3xFLAG-YAP1-S127A

    Plasmid

    #42239

    Depositor

    William Pu

    Article

    von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Gateway

    Tags

    3xFLAG

    Mutation

    Serine 127 changed to alanine

    Promoter

    none

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_UdgX-APOBEC1-UdgX-NG-nCas9

    Plasmid

    #163562

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAR100

    Plasmid

    #180377

    Purpose

    pAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).

    Depositor

    Alec Redwood

    Article

    Sooda et al Commun Biol. 2022 Feb 16;5(1):133. doi:

    Inserts

    NFAT-Luciferase
    hCD8A
    

    Expression

    Bacterial and Mammalian

    Promoter

    hEF1-HTLV and minimal promoter and an NFAT respon…

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. proISG15 (2-165)

    Plasmid

    #110762

    Purpose

    Bacterial expression of proISG15

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Tags

    N-His6-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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