We narrowed to 15,824 results for: LEA

1. pBK092

   Plasmid

   #183146

   Purpose

   pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expression

   Depositor

   Daan Swarts

   Article

   Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

   Insert

   CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDisplay-GRAB_CRF1.0

   Plasmid

   #208655

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. OCT4-eGFP-PGK-Puro

   Plasmid

   #31937

   Purpose

   Oct4 targeting donor vector

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

   Insert

   OCT4 (POU5F1 Human)
   

   Use

   Targeting donor

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO_AU1.C.HA_mCherry-NLS

   Plasmid

   #178209

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   AU1.C.HA and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   eF1a

   Available since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO_Ollas.S.V5_mCherry-NLS

   Plasmid

   #178211

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   Nuclear Localization Signal and Ollas.S.V5

   Expression

   Mutation

   Promoter

   eF1a

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-DroshaS300/302A

   Plasmid

   #62530

   Purpose

   Serines 300 and 302 of Drosha are mutated to alanines

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   human Drosha with serines 300 and 302 changed alanines (DROSHA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed serines 300 and 302 to alanines

   Promoter

   Available since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pclbw-opa1(isoform 1)-myc

   Plasmid

   #62845

   Purpose

   Mammalian Expression of myc-tagged Opa1 isoform1

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   mmOPA1isoform1-myc (Opa1 Mouse)
   

   Use

   Retroviral

   Tags

   9xMyc

   Expression

   Mammalian

   Mutation

   Promoter

   cmv-bactin

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin

   Plasmid

   #133025

   Purpose

   Expresses EGFP-tagged tail anchor sequence of monoamine oxidase A

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Monoamine oxidase type A (MAOA Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-488

   Promoter

   Human elongation factor 1 alpha

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-Tet-On-Arl13b-RhoA sensor

   Plasmid

   #189613

   Purpose

   Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   Arl13b
   

   Use

   Tags

   GFP and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   Tet3G

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAR100

    Plasmid

    #180377

    Purpose

    pAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).

    Depositor

    Alec Redwood

    Article

    Sooda et al Commun Biol. 2022 Feb 16;5(1):133. doi:

    Inserts

    NFAT-Luciferase
    hCD8A
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    hEF1-HTLV and minimal promoter and an NFAT respon…

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKH3-TRIM28-C65A/C68A

    Plasmid

    #92199

    Purpose

    Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    Tif1b (TRIM28 Synthetic, Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Cysteines 65 and 68 to Alanines.

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5-Myc-PSK1-beta

    Plasmid

    #197121

    Purpose

    Expression of MYC-tagged PSK1-beta in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Zihni et al J Biol Chem. 2007 Mar 2;282(9):6484-93.

    Insert

    TAOK2 variant beta (aa1-1049) (TAOK2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Wild Type variant beta

    Promoter

    SV40

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEN017-SpCas9-hiNLS-t-M4

    Plasmid

    #234455

    Purpose

    Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEN019-SpCas9-hiNLS-t-M1M4

    Plasmid

    #234456

    Purpose

    Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M1M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-hSyn-DIO-mCReP

    Plasmid

    #167503

    Purpose

    Conditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)

    Depositor

    Nicole Calakos

    Article

    Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

    Insert

    Ppp1r15b (Ppp1r15b Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHis-LMW_FGF2

    Plasmid

    #157657

    Purpose

    Expresses FGF2 in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    changed Cystein 211,229 to serine, deleted amino …

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-FLEX-ArgiNLS-AausFP1

    Plasmid

    #220602

    Purpose

    Cre-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    AausFP1
    

    Use

    AAV and Cre/Lox

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSynapsin_NLS_GFP-P2A_SNAP_GluA2

    Plasmid

    #170455

    Purpose

    Expresses SNAP tagged GluA2 in neuronal cells

    Depositor

    Shigeki Watanabe

    Article

    Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

    Insert

    GluA2 (Gria2 Mouse)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-GFP-EphrinB3-Flag

    Plasmid

    #155012

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    EphrinB3 (EPHB3 Human)
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHtrA-TEV-His12

    Plasmid

    #137036

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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