We narrowed to 14,647 results for: LEA
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Plasmid#119830PurposeProduction of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluh
TagsGSTExpressionBacterialMutationE25APromoterT7Available sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-FLEX-ArgiNLS-AausFP1
Plasmid#220602PurposeCre-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.DepositorInsertAausFP1
UseAAV and Cre/LoxTagsArgiNLSExpressionMammalianPromoterCAGAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1313
Plasmid#217955PurposeExpression of human H3.1 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHistone H3.1 (H3C1 Human)
TagseGFPExpressionMammalianPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-5-enzymatic-domain
Plasmid#214306PurposeProtein overexpression in bacteriaDepositorAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pminiTol2-HuC-NE2m
Plasmid#208688PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m pan-neuronally in zebrafishDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2m
UseCloning vectorPromoterHucAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-LbCas12a-Plus-mCherry
Plasmid#191230PurposeTo express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLbCas12a-Plus-P2A-mCherry
UseCRISPRMutationD156R, R883K, R887AAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMAH-ER-bapaUGAc
Plasmid#201674Purposeboronic acid- and peptide-assisted UDP-GlcNAc sensor in ERDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertER-bapaUGAc
TagsER leading sequence and ER retention sequenceExpressionMammalianPromoterCMV SP6Available sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-N-TEV-HAT
Plasmid#202598PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cellDepositorTypeEmpty backboneTagsTEV cleavable site (ENLYFQG) and poly-histidine a…ExpressionMammalianPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5mycGW-Eps8L2
Plasmid#196542PurposeExpression of myc-Eps8L2-FLDepositorAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_VNp-mNeongreen_CydAB-mCherry
Plasmid#182391PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusionsDepositorInsertsVNp-mNeongreen-His
cydAB-mCherry
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FnR9-10
Plasmid#182567PurposeHuman fibronectin fragment, type-III repeats 9-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 9-10
TagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379AAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHisIHFαIHFβ
Plasmid#149384PurposeExpression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbetaDepositorInsertsPseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
Pseudomonas aeruginosa PA14 IHFbeta
Tags6xHis tag and HRV3C cleavage siteExpressionBacterialPromoterT7Available sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMVTO-SEAP-26SFur-B2AD-tvmvs-KKMP
Plasmid#179645PurposeTVMVP-inducible single TM SEAP RELEASE constructDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSEAP RELEASE
ExpressionMammalianAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjVASA-tdTomato
Plasmid#160872PurposeMarmoset TargetingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttdTomato
UseMarmoset targetingAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXR1AtR2B2NtS1
Plasmid#160879PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
ExpressionBacterialPromoterrbcS-S1: pBAD, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGlul
Plasmid#119832PurposeProduction of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGlul
TagsGSTExpressionBacterialMutationT92APromoterT7Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMU103
Plasmid#73934PurposeThis is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.DepositorInsertbar and GUS cassettes
ExpressionPlantPromoter35SAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
5THA (Gemin5)
Plasmid#101890PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertGemin5 (GEMIN5 Human)
UseBaculovirus expressionTags6xHis, Thrombin cleavagePromoterpolyhedrinAvailable sinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
5WBN (HDAC6)
Plasmid#101267PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHDAC6 (HDAC6 Human)
TagsHexahistidine tag with Thrombin cleavageExpressionBacterialPromoterT7Available sinceOct. 2, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSUPER-retro-puro-shNup133-1
Plasmid#87334PurposeTo express shRNA against human Nup133DepositorInsertshRNA against human Nup133
UseRNAiExpressionMammalianPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only