We narrowed to 19,051 results for: Tec;

1. ND4.2-Left TALE-G1397-N-DddA11-mCherry

   Plasmid

   #179682

   Purpose

   Expression of base editor in mammalian cells and for cell sorting

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
   

   Expression

   Mammalian

   Mutation

   S1330I A1341V N1342S E1370K T1380I

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGM1190

   Plasmid

   #69994

   Purpose

   OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

   Depositor

   Günther Muth

   Article

   Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

   Inserts

   aac
   tsr
   rep
   sso
   TipA promoter
   dso
   

   Expression

   Bacterial

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. CRISPR-StAR4BN (Lenti)

   Plasmid

   #222692

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PGK without BsmBI cutsite

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EFS-DIO-GRAB_OT1.0

   Plasmid

   #185387

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   GPCR activation based oxytocin sensor GRAB_OT1.0
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. VPX-mRNA

   Plasmid

   #227679

   Purpose

   plasmid for VPX mRNA IVT

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

   Insert

   VPX (HIV2gp3 )
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2307-Tier1-2xcHS4-PPGK-NLuc_TB

   Plasmid

   #169550

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   cHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBG-ttLbUV2

   Plasmid

   #231386

   Purpose

   LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.

   Depositor

   Qi-Jun Chen

   Article

   Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

   Insert

   ttLbCas12a Ultra V2
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D156R and E795L

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCH45 (multiAsCas12a-KRAB lenti)

   Plasmid

   #217330

   Purpose

   Lentiviral expression of multiAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   multiAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Mutation

   R1226A/E174R/S542R/K548R

   Promoter

   UCOE-SFFV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PEmaxNG-_2star_IVT

   Plasmid

   #227678

   Purpose

   plasmid for SpCas9-NG-PEmax** mRNA IVT

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

   Insert

   NG-PEmax_2_star
   

   Use

   CRISPR

   Tags

   no

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid

    #181792

    Purpose

    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9-NG

    Plasmid

    #138566

    Purpose

    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CABE-T1.17

    Plasmid

    #193260

    Purpose

    Expression of CABE-T1.17 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T1.17
    

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. CABE-T2.9

    Plasmid

    #193264

    Purpose

    Expression of CABE-T2.9 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T2.9
    

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP-Actin-7

    Plasmid

    #56421

    Purpose

    Localization: Actin, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Actin (ACTB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. CRISPR-StAR4 (Retro)

    Plasmid

    #222690

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Mutation

    PGK without BbsI cutsite

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AA364

    Plasmid

    #215956

    Purpose

    Fragmid fragment: (guide cassette) epegRNA expression

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    dU6:3_v1; BsmBI_v14; BsmBI_v15; evopreQ1_v1 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. HIV-1-GFP ΔEnv

    Plasmid

    #217437

    Purpose

    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu.

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-HaloAKAR2.1-CLC

    Plasmid

    #216492

    Purpose

    Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) targeted to clathrin coated pits

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.1-CLC
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

    Plasmid

    #164611

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Expression

    Mammalian

    Mutation

    S383T

    Promoter

    CMV

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA

    Plasmid

    #205317

    Purpose

    All-in-one AAV vector expressing ISYmu1 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISYmu1 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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