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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,777 results for: BLI

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Showing: 6961 - 6980 of 9777 results

  1. pelBHisTEV

    Plasmid
    #157741
    Purpose
    empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Tags
    PelB signal sequence + His10-tag + TEV protease c…
    Expression
    Bacterial
    Promoter
    T7lac
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    157741-plasmid-map-sequence-id-334005

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  2. SmBiT-ADCY8 BiBiT-Ready Vector

    Plasmid
    #237115
    Purpose
    Express SmBiT-ADCY8 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    ADCY8 (ADCY8 Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237115-plasmid-map-sequence-id-474701

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  3. mEmerald-ERBB2-N-18

    Plasmid
    #62755
    Purpose
    Localization: Membrane Receptor, N1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    ERBB2 (ERBB2 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62755-plasmid-map-sequence-id-406429

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  4. pBABE.puro/TO/Flag/HuR.wt

    Plasmid
    #110346
    Purpose
    Doxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG tag
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    gag
    Available Since
    July 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    110346-plasmid-map-sequence-id-215276

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  5. AICSDP-117:POLR2A-mEGFP

    Plasmid
    #164500
    Purpose
    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A
    Depositor
    Allen Institute for Cell Science
    Insert
    POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Feb. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    164500-plasmid-map-sequence-id-324900

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  6. NanoLuc-EGR2 Fusion Vector

    Plasmid
    #237171
    Purpose
    Express NanoLuc(R)-EGR2 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    EGR2 (EGR2 Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237171-plasmid-map-sequence-id-473334

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  9. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid
    #174138
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174138-plasmid-map-sequence-id-341915

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  10. Hs.KRAS4a

    Plasmid
    #83166
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83166-plasmid-map-sequence-id-158129

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  11. Lamin A (R377H)-mRFP

    Plasmid
    #124270
    Purpose
    Lamin A mutant (R377H), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R377H) (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R377H)
    Available Since
    July 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124270-plasmid-map-sequence-id-251113

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  12. pIRESN2_GFP_YTA_Champ1_328-354

    Plasmid
    #105639
    Purpose
    Expresses fragment (amnio acids 328-354) of human Champ1 protein tagged with GFP
    Depositor
    Thanos Halazonetis
    Insert
    Champ1 (CHAMP1 Human)
    Tags
    GFP YTA
    Expression
    Mammalian
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105639-plasmid-map-sequence-id-203817

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  13. pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50467
    Purpose
    Gi-coupled hM4D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has Service
    AAV2 and AAV8
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50467-plasmid-map-sequence-id-199198

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  14. pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50471
    Purpose
    Gi-coupled hM4D-IRES-mCitrine under the control of GFAP promoter
    Depositor
    Bryan Roth
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50471-plasmid-map-sequence-id-75491

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  15. 6xO/S luc

    Plasmid
    #69445
    Purpose
    detection/assessment of OCT4 and SOX2 transcription factor activity
    Depositor
    Lisa Dailey
    Insert
    minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
    Use
    Luciferase
    Expression
    Mammalian
    Available Since
    May 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    69445-plasmid-map-sequence-id-259015

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  18. pAG416-GAL-FUS-G156E

    Plasmid
    #181716
    Purpose
    Galactose inducible expression of FUS-G156E
    Depositor
    Alejandro Chavez
    Insert
    FUS (FUS Human)
    Expression
    Yeast
    Mutation
    G156E
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    181716-plasmid-map-sequence-id-430956

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  19. PRDX6A-c001

    Plasmid
    #175474
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    PRDX6 (PRDX6 Human)
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Mutation
    See Depositor Comments
    Available Since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    175474-plasmid-map-sequence-id-353175

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  20. pAAV-hSyn-GluA2-scFv-mNeon-KDEL

    Plasmid
    #230057
    Purpose
    AAV plasmid that expresses a a single chain variable fragment (scFv) for anti- GluA2 with a C-terminal mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA2 single chain variable fragment (scFv)
    Use
    AAV
    Tags
    ER retrieval motif (KDEL) and mNeon
    Expression
    Mammalian
    Promoter
    human synapsin
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230057-plasmid-map-sequence-id-457175

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Showing: 6961 - 6980 of 9777 results