We narrowed to 10,193 results for: Pol;

1. pCMV-FLAG-CHD5-Y619E-FRAG

   Plasmid

   #68871

   Purpose

   Mammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutation

   Depositor

   Adrian Bracken

   Article

   Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

   Insert

   CHD5 (CHD5 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   331-672aa, Y619E

   Promoter

   CMV

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pShuttle-CMV-Flag-SREBP-1c

   Plasmid

   #237348

   Purpose

   Adenoviral-SREBP-1c cleavage-activation reporter system

   Depositor

   Gerald Höfler

   Article

   Pena de la Sancha et al Commun Biol. 2025 Apr 9;8(1):588. doi: 1

   Insert

   SREBF1 (SREBF1 Human)
   

   Use

   Adenoviral and Mouse Targeting

   Tags

   Flag tag

   Promoter

   CMV

   Available Since

   Nov. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF-1α-Venus-Nup107-FRT

   Plasmid

   #247344

   Purpose

   Expresses Venus-NUP107 under EF1 promoter and can be integrated into FRT site

   Depositor

   Kazuhiro Maeshima

   Article

   Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

   Insert

   Nup107 (NUP107) (NUP107 Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMAPLe4 PCMTD1 T62 H67 M68

   Plasmid

   #246506

   Purpose

   Recombinant protein expression of PCMTD1 T62 H67 M68

   Depositor

   Steven Clarke

   Article

   Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

   Insert

   Protein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Residue 312 is Isoleucine in this plasmid but occ…

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB_rtTA_BsmB-HNRNPK_sgRNA_4

   Plasmid

   #242899

   Purpose

   PiggyBac cargo vector with HNRNPK sgRNA 4 for dox-inducible knockdown

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

   Insert

   HNRNPK (Hnrnpk Mouse)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Sept. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB_rtTA_BsmB-HNRNPK_sgRNA_1

   Plasmid

   #242897

   Purpose

   PiggyBac cargo vector with HNRNPK sgRNA 1 for dox-inducible knockdown

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

   Insert

   HNRNPK (Hnrnpk Mouse)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB_rtTA_BsmB-HNRNPK_sgRNA_3

   Plasmid

   #242898

   Purpose

   PiggyBac cargo vector with HNRNPK sgRNA 3 for dox-inducible knockdown

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

   Insert

   HNRNPK (Hnrnpk Mouse)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-4T-3-GST-Tev-Af1521(K35E/Y145R)-myc

   Plasmid

   #196241

   Purpose

   N-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminus

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   N-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag
   

   Tags

   GST tag and myc tag

   Expression

   Bacterial

   Mutation

   amino acid 35 lysine is replaced with glutamic ac…

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. TCTN3 minigene (pAVA3939)

   Plasmid

   #239345

   Purpose

   Expresses TCTN3 minigene (EFS-6xMyc-TCTN3-Ex10-I10-Ex11-copGFP-3xFLAG)

   Depositor

   Andrei Alexandrov

   Article

   Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

   Insert

   EFS-6xMyc-TCTN3-Ex10-I10-Ex11-copGFP-3xFLAG-BGH polyA (TCTN3 Human)
   

   Tags

   3xFLAG, 6xMyc, and CopGFP

   Expression

   Mammalian

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Rab11 single guide RNA

    Plasmid

    #229683

    Purpose

    guide RNA for Rab11 tagging at the N-terminus in pX459

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    Rab11 guide (RAB11A Human)
    

    Use

    CRISPR

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #223323

    Purpose

    Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

    Depositor

    Jonathan Arias

    Article

    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

    Insert

    SpCas9 and sgRNA-T2 (cas9 )
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG8Ha-EWS_RRM(359-447)

    Plasmid

    #188045

    Purpose

    Expresses His8-EWS_RRM(359-447) with a TEV site for removal of the His8 tag.

    Depositor

    David Libich

    Article

    Selig et al Biopolymers. 2023 May;114(5):e23536. doi

    Insert

    EWSR1 (EWSR1 Human)
    

    Tags

    His8

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-358, 446-656

    Promoter

    T7

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG8Ha-EWS_RRM-RGG(359-513)

    Plasmid

    #188046

    Purpose

    Expresses His8-EWS_RRM-RGG(359-513) with a TEV site for removal of the His8 tag.

    Depositor

    David Libich

    Article

    Selig et al Biopolymers. 2023 May;114(5):e23536. doi

    Insert

    EWSR1 (EWSR1 Human)
    

    Tags

    His8

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-358, 514-656

    Promoter

    T7

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEmal10H-MBP-EWSR1-449-513

    Plasmid

    #199439

    Purpose

    Expresses RG2 (the second RG-rich region, 449-513) of EWS

    Depositor

    David Libich

    Article

    Selig et al Biopolymers. 2023 May;114(5):e23536. doi

    Insert

    EWSR1 (EWSR1 Human)
    

    Tags

    His10 and MBP

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-448, 514-656

    Promoter

    T7

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Click editor utilizing mSA for template recruitment - pCMV-T7-mSA-nCas9-EcKlenow (JO1391)

    Plasmid

    #217802

    Purpose

    Variant CE1 construct with mSA for template recruitment, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    mSA-linker-SV40NLS-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Unfused click editor construct; mSA-EcKlenow fusion - pCMV-T7-mSA-EcKlenow (JO1401)

    Plasmid

    #217806

    Purpose

    Unfused click editor construct expressing mSA-EcKlenow, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    mSA-linker-SV40NLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. HUHe variant click editor (CE1) - pCMV-T7-StCV-nCas9-EcKlenow (JO1269)

    Plasmid

    #217790

    Purpose

    Variant CE1 construct with StCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    StCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HUHe variant click editor (CE1) - pCMV-T7-CaCV-nCas9-EcKlenow (JO1261)

    Plasmid

    #217791

    Purpose

    Variant CE1 construct with CaCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    CaCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HUHe variant click editor (CE1) - pCMV-T7-Chimp17-nCas9-EcKlenow (JO1275)

    Plasmid

    #217792

    Purpose

    Variant CE1 construct with Chim HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Chimp17-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HUHe variant click editor (CE1) - pCMV-T7-GoCV-nCas9-EcKlenow (JO1277)

    Plasmid

    #217793

    Purpose

    Variant CE1 construct with GoCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    GoCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge