We narrowed to 35,954 results for: Spr;
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Plasmid#111445PurposeSolo vector pV1382 + sgScADE2DepositorInsertCaCas9/sgScADE2/stop-codon repair
UseCRISPRExpressionYeastAvailable SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIK198
Plasmid#65629Purpose"flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.DepositorTypeEmpty backboneUseCRISPRExpressionWormPromoterU6Available SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_MLX
Plasmid#86277PurposeDonor vector for 3' FLAG tag of human MLXDepositorAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133D2.0
Plasmid#99894PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterOsU3Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131D2.0
Plasmid#100044PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterOsU3Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_TEAD1
Plasmid#86291PurposeDonor vector for 3' FLAG tag of human TEAD1DepositorAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB6681
Plasmid#106157PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_K1082A
Plasmid#91863PurposeBacterial expression for Cas13aDepositorInsertLbuCas13a (LbuC2c2) K1082A
TagsHis6-MBP-TEV siteExpressionBacterialMutationK1082AAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AaCas12b(D977A)-2AeGFP
Plasmid#121962PurposeMammalian expression, Transcription regulation, deactivated AaCas12bDepositorInsertCatalytically deactivated AaCas12b
Tags3xHA tagExpressionMammalianMutationD977AAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTFAP2C.1.0-gDNA
Plasmid#113792PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFAP2CDepositorAvailable SinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas2D
Plasmid#82375PurposedCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACGDepositorInsertdCas9
PromoterPEZ3Available SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZGPAT
Plasmid#86276PurposeDonor vector for 3' FLAG tag of human ZGPATDepositorAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-p65(150-261)-RTA(125-190)
Plasmid#99674PurposeExpresses dSp Cas9 fused to combination of truncated activation domainsDepositorArticleInsertdCas9
TagsVP64-P65(151-261)-RTA(125-190)ExpressionMammalianMutationdead Cas9Available SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mEGFP-N
Plasmid#113746PurposeGateway multi-site entry clone for second position mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP
UseCRISPR; Gateway entry vectorTagsmEGFPAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0272_pET30_pCat-tetR-Term-ptetA-Rluc-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI
Plasmid#116956PurposeExpresses FsRT-Cas1-Cas2 under pT7lac and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
ExpressionBacterialPromoterT7Available SinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mEGFP-C
Plasmid#113749PurposeGateway multi-site entry clone for second position mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP
UseCRISPR; Gateway entry vectorTagsmEGFPAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mRuby-C
Plasmid#113755PurposeGateway multi-site entry clone for second position mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2
UseCRISPR; Gateway entry vectorTagsmRuby2Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmRuby-C
Plasmid#113757PurposeGateway multi-site entry clone for second position 3x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags3xmRuby2Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_SOX5
Plasmid#86292PurposeDonor vector for 3' FLAG tag of human SOX5DepositorAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF644
Plasmid#86264PurposeDonor vector for 3' FLAG tag of human ZNF644DepositorAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only