We narrowed to 150,751 results for: ins

1. pET28a_6HT-TBP6.7(R52A)

   Plasmid

   #112729

   Purpose

   52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R52A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R52A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMGF169

   Plasmid

   #96996

   Purpose

   ish1 mCherry insertion cassette with G418 resistance gene

   Depositor

   Adam Frost, Wesley Sundquist

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   ish1 (ish1 Fission Yeast)
   

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   ish1 C-terminal domain fused with mCherry + G418 …

   Promoter

   N/A

   Available Since

   Aug. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. KCC4_HUMAN_D0

   Plasmid

   #79744

   Purpose

   This plasmid encodes the kinase domain of KCC4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KCC4 (SLC12A7 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. KS6A1_HUMAN_D1

   Plasmid

   #79720

   Purpose

   This plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KS6A1 (RPS6KA1 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. STK10_HUMAN_D0

   Plasmid

   #79722

   Purpose

   This plasmid encodes the kinase domain of STK10. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   STK10 (STK10 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. Hy_pMT Laccase2 MCS-ZKSCAN1 548-847

   Plasmid

   #69885

   Purpose

   Expresses a miniature version of the ZKSCAN1 circular RNA in Drosophila

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1 (stw, ZKSCAN1 Human)
   

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUAST-HA, Pbl-A DH domain

   Plasmid

   #69794

   Purpose

   Pebble isoform A, DH domain, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

   Depositor

   Arno Muller

   Article

   van Impel et al Development. 2009 Mar;136(5):813-22. doi

   Insert

   Pebble DH domain
   

   Use

   P element-based puast vector for gal4-regulated e…

   Tags

   HA-Tag

   Expression

   Insect

   Mutation

   Contains only the Pebble DH domain

   Promoter

   hsp70

   Available Since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pZM3[I:FruR-L,I:RbsR-L]

   Plasmid

   #60777

   Purpose

   Contains PI driving expression FruR-L, and PI driving expression of RbsR-L.

   Depositor

   Matthew Bennett

   Article

   Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

   Inserts

   FruR-L
   RbsR-L
   

   Mutation

   LacI/GalR repressor chimera. LacI DBD with FruR L…

   Available Since

   July 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-p53 (1-73) (K24N)

   Plasmid

   #62079

   Purpose

   Expression of human p53 (1-73) (K24N) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

   Insert

   p53 (TP53 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-73; K24N

   Promoter

   T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

10. BRCA1-(219-408)

    Plasmid

    #35067

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 219-408

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pTriEx-mCherry-PA-Rac1

    Plasmid

    #22027

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Tags

    6xHis and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIDS-LSD1fl

    Plasmid

    #109157

    Purpose

    Encodes human full-length LSD1 to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human full-length LSD1, sequence-optimized for insect cells (KDM1A Human)
    

    Expression

    Insect

    Promoter

    p10

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRE-BI-osTIR1

    Plasmid

    #207840

    Purpose

    Inducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

    Depositor

    Michaela Mueller-McNicoll

    Article

    Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

    Inserts

    osTIR
    mCherry
    mAID_-VHH-GFP4
    

    Tags

    3X HA-Tag and weak NLS

    Expression

    Mammalian

    Mutation

    All K mutated to R

    Promoter

    TRE3G BI promoter

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-PR rep/cap

    Plasmid

    #197565

    Purpose

    encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery

    Depositor

    Casey Maguire

    Article

    Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089

    Inserts

    AAV Rep genes
    AAV9 VP1 Cap gene with PR insert
    

    Use

    AAV

    Mutation

    contains 7mer insert PRPPSTH between amino acids …

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTTCC

    Plasmid

    #164264

    Purpose

    Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

    Depositor

    Gudmundur Hreggvidsson

    Article

    Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

    Inserts

    Cas9
    gRNA scaffold
    

    Expression

    Bacterial

    Promoter

    Pcel and trc

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid

    #140001

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-UBC-XRI-HA

    Plasmid

    #178056

    Purpose

    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    XRI-HA
    

    Use

    AAV

    Tags

    HA-MBP

    Expression

    Mammalian

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid

    #139998

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET32a-hHBEGF

    Plasmid

    #199234

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Proheparin-binding EGF-like growth factor (HBEGF Human)
    

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-CAAX

    Plasmid

    #86056

    Purpose

    A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Homo sapiens Harvey rat sarcoma (HRAS Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains the region from 825-857 of HRAS as a far…

    Promoter

    CMV

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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