We narrowed to 7,545 results for: alp;

1. pIREShyg-PA-GFP-alpha Tubulin

   Plasmid

   #12296

   Depositor

   Patricia Wadsworth

   Article

   Tulu et al Curr Biol. 2003 Oct 28. 13(21):1894-9.

   Insert

   alpha Tubulin (TUBA1B Human)
   

   Use

   Tags

   PA-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO-GAlpha15-RLuc8

   Plasmid

   #140984

   Purpose

   Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlpha15-RLuc8 (GNA15 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5/FRT/TO-GAlphaoA-RLuc8

   Plasmid

   #140976

   Purpose

   Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaoA-RLuc8 (GNAO1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5/FRT/TO-GAlphai3-RLuc8

   Plasmid

   #140975

   Purpose

   Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphai3-RLuc8 (GNAI3 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-Flag-PGC-1alpha1

   Plasmid

   #45501

   Depositor

   Bruce Spiegelman, Jorge Ruas

   Article

   Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

   Insert

   PGC-1alpha Isoform1 (Ppargc1a Mouse)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Q339K relative to NM_008904

   Promoter

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-hSyn-Galpha-oA ONE-GO

   Plasmid

   #204335

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   hSyn Galpha-oA ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. Stat1 alpha pRC/CMV

   Plasmid

   #8690

   Depositor

   Jim Darnell

   Article

   Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

   Insert

   STAT1 alpha (STAT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

8. FusX alphaDdCBE DddA6-11 1397C

   Plasmid

   #221938

   Purpose

   Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6/11 1397C

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   3xFLAG

   Expression

   Mutation

   TALE: Q231R, W232G, S233A | DddA: T1413I

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FusX alphaDdCBE DddA6 1397N

   Plasmid

   #221939

   Purpose

   Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6 1397N

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   3xFLAG

   Expression

   Mutation

   TALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FusX alphaDdCBE DddA11 1397N

    Plasmid

    #221940

    Purpose

    Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA11 1397N

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    3xFLAG

    Expression

    Mutation

    TALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-EF1alpha-soCoChR-tdTomato

    Plasmid

    #107714

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-tdTomato
    

    Use

    AAV

    Tags

    KA2 and tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-myc-PDGFRalpha-V242K

    Plasmid

    #136436

    Purpose

    Mammalian expression plasmid for c-myc-tagged PDGFRA (V242K mutant)

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRalpha (PDGFRA Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    V242K (disrupts PDGF binding, but still binds HCM…

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiCRISPR v2-sgAMPK alpha1 clone1

    Plasmid

    #162119

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)

    Plasmid

    #160607

    Purpose

    TU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.

    Depositor

    Diego Orzaez

    Insert

    CUP2Gal4AD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LMP AMPK alpha1 hp1

    Plasmid

    #89489

    Purpose

    shRNA against AMPK alpha1 in mouse/human to knock down protein expression

    Depositor

    Russell Jones

    Article

    Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

    Insert

    hairpin against mouse/human AMPKalpha1 (PRKAA1, Prkaa1 Mouse, Human)
    

    Use

    Mouse Targeting and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (The GFP-Puro is from a PGK promoter; the shRN…

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA5/FRT/TO-GAlpha11-RLuc8

    Plasmid

    #140983

    Purpose

    Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha11-RLuc8 (GNA11 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pscALPS-HIV-1 Group P-vpr

    Plasmid

    #115837

    Purpose

    Encodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    HIV1 Group P Vpr (HQ179987)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBSSK WT p85 alpha flag

    Plasmid

    #13429

    Depositor

    Ronald Kahn

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

    Insert

    p85 alpha (PIK3R1 Human)
    

    Use

    Tags

    flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V

    Plasmid

    #66789

    Purpose

    Expression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoter

    Depositor

    Lotte Pedersen

    Article

    Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

    Insert

    PDGFRalpha (Pdgfra Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D842V (constitutively active)

    Promoter

    EF1a

    Available since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pEJS887_pTetR-P2A-BFPnls/sgAlpha

    Plasmid

    #108651

    Purpose

    Alpha satellite repeats targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Alpha satellite repeats targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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