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CA-0211 CMVTO-hIL2-rens-del.2

Plasmid

#231228

Purpose

CleaveIL-2_loop3_del

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL2-rens-del.2 (IL2 Human)

Expression

Mammalian

Mutation

L80F, R81D, L85V, I86V, I92F

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0201 CMVTO-hIL6-rens.1

Plasmid

#231197

Purpose

CleaveIL-6

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL6-rens.1 (IL6 Human)

Expression

Mammalian

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0212 CMVTO-hIL2-rens-del.3

Plasmid

#231229

Purpose

CleaveIL-2_loop2_del

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL2-rens-del.3 (IL2 Human)

Expression

Mammalian

Mutation

L80F, R81D, L85V, I86V, I92F

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0033 CMVTO-hIL6

Plasmid

#231192

Purpose

Human IL-6

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Human IL-6 (IL6 Human)

Expression

Mammalian

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pWP003

Plasmid

#198803

Purpose

Drives specific expression in the URX neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pflp-8-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHW489

Plasmid

#198771

Purpose

Drives specific expression in the ALA neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pflp-24-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/MACROD1

Plasmid

#172578

Purpose

For generation of FlpIn lines with untagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Tags

none

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1-d80

Plasmid

#114025

Purpose

Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-d80-HEC1 (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 1 to 80 deleted

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-d80

Plasmid

#114030

Purpose

Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-d80-HEC1 (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 1 to 80 deleted

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-8A/S15D

Plasmid

#114031

Purpose

Inducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1-8A/S15D (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 8A/S15D

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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CA-0039 CMVTO-hIL10 (Fused Dimer)

Plasmid

#231187

Purpose

Fused human IL-10 dimer

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Fused human IL-10 dimer (IL10 Human)

Expression

Mammalian

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pB-CAG-FLEX-F14F15-BFP-(mCherry)'

Plasmid

#201831

Purpose

piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Inserts

CAG
BFP

Expression

Mammalian

Promoter

CAG

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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CMVTO-KRAS

Plasmid

#179653

Purpose

KRAS protein

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

KRAS (KRAS Human)

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CA-0059 CMVTO-SumIL2-rens-5GS-IL2Rb

Plasmid

#231224

Purpose

CageIL-2 (C-term 25 AA)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

SumIL2-rens-5GS-IL2Rb (IL2 Human)

Expression

Mammalian

Mutation

L80F, R81D, L85V, I86V, I92F

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-KA2N-EGFP

Plasmid

#114378

Purpose

expresses Flpo recombinase-dependent GtACR2-KA2N, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-KA2N-EGFP (NEWENTRY )

Use

AAV

Tags

EGFP and KA2N

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC

Plasmid

#114376

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv2.1C-TlcnC (NEWENTRY )

Use

AAV

Tags

EYFP, Kv2.1C, and TlcnC

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-MBD

Plasmid

#114371

Purpose

expresses Flpo recombinase-dependent GtACR2-MBD, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-MBD (NEWENTRY )

Use

AAV

Tags

EYFP and MBD

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-TlcnC

Plasmid

#114374

Purpose

expresses Flpo recombinase-dependent GtACR2-TlcnC, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-TlcnC (NEWENTRY )

Use

AAV

Tags

EYFP and TlcnC

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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CA-0034 CMVTO-hIL6-rens-hIL6Ra_M4

Plasmid

#231217

Purpose

C-CageIL-6

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL6-rens-hIL6Ra_M4 (IL6 Human)

Expression

Mammalian

Mutation

A228D, N230D, H280S, D281V

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0200 CMVTO-hIL6Ra_M4-rens-hIL6

Plasmid

#231193

Purpose

CageIL-6 (N-term)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL6Ra_M4-rens-hIL6 (IL6 Human)

Expression

Mammalian

Mutation

A228D, N230D, H280S, D281V

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0057 CMVTO-SumIL2-rens-2GS-IL2Rb

Plasmid

#231222

Purpose

CageIL-2 (C-term 10 AA)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

SumIL2-rens-2GS-IL2Rb (IL2 Human)

Expression

Mammalian

Mutation

L80F, R81D, L85V, I86V, I92F

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0046 CMVTO-hIL10dimer-rens-IL10Ra

Plasmid

#231188

Purpose

Fused cageIL-10-dimer

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL10dimer-rens-IL10Ra (IL10 Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0062 CMVTO-SumIL2-3GS-rens-IL2Rb

Plasmid

#231220

Purpose

CageIL-2 (N-term 15 AA)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

SumIL2-3GS-rens-IL2Rb (IL2 Human)

Expression

Mammalian

Mutation

L80F, R81D, L85V, I86V, I92F

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0064 CMVTO-SumIL2-noGS-rens-IL2Rb

Plasmid

#231218

Purpose

CageIL-2 (N-term 0 AA)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

SumIL2-noGS-rens-IL2Rb (IL2 Human)

Expression

Mammalian

Mutation

L80F, R81D, L85V, I86V, I92F

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CA-0165 CMVTO-mono-hIL10 (R5A11M)-rens

Plasmid

#231196

Purpose

CleaveIL-10

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

mono-hIL10 (R5A11M)-rens (IL10 Human)

Expression

Mammalian

Mutation

N36I, N110I, K117N, F129L

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CA253 CMVTO-rens-3GS-IL10Ra-c-myc

Plasmid

#231213

Purpose

Cleaved IL-10Ra-myc

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

rens-3GS-IL10Ra-c-myc (IL10 Human)

Expression

Mammalian

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0202 CMVTO-hIL6-rens.1_Del

Plasmid

#231225

Purpose

CleaveIL-6_Del

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL6-rens.1_Del (IL6 Human)

Expression

Mammalian

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CA-0045 CMVTO-mono-hIL10 (R5A11M)

Plasmid

#231190

Purpose

Mutant human IL-10 monomer

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

mono-hIL10 (R5A11M) (IL10 Human)

Expression

Mammalian

Mutation

N36I, N110I, K117N, F129L

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CA-0058 CMVTO-SumIL2-rens-3GS-IL2Rb

Plasmid

#231223

Purpose

CageIL-2 (C-term 15 AA)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

SumIL2-rens-3GS-IL2Rb (IL2 Human)

Expression

Mammalian

Mutation

L80F, R81D, L85V, I86V, I92F

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CA-0063 CMVTO-SumIL2-5GS-rens-IL2Rb

Plasmid

#231221

Purpose

CageIL-2 (N-term 25 AA)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

SumIL2-5GS-rens-IL2Rb (IL2 Human)

Expression

Mammalian

Mutation

L80F, R81D, L85V, I86V, I92F

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CA-0061 CMVTO-SumIL2-2GS-rens-IL2Rb

Plasmid

#231219

Purpose

CageIL-2 (N-term 10 AA)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

SumIL2-2GS-rens-IL2Rb (IL2 Human)

Expression

Mammalian

Mutation

L80F, R81D, L85V, I86V, I92F

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-syn-F14F15S-jGCaMP7s

Plasmid

#178514

Purpose

AAV vector for Flpo recombinase dependent jGCaMP7s

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

jGCaMP7s

Use

AAV

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO/MACROD2

Plasmid

#172579

Purpose

For generation of FlpIn lines with untagged MACROD2

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD2 (MACROD2 Human)

Tags

none

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-RBDK65E-R89L-nTEVp

Plasmid

#179670

Purpose

Mutant Ras Binding Domain split-nTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Tags

nTEVP

Expression

Mammalian

Mutation

K65E, R89L

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-RBDK65E-R89L-cTEVp

Plasmid

#179671

Purpose

Mutant Ras Binding Domain split-cTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Tags

cTEVP

Expression

Mammalian

Mutation

K65E, R89L

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-HRAS-G12V

Plasmid

#179652

Purpose

HRAS active mutant (G12V mutation)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

HRAS (HRAS Human)

Expression

Mammalian

Mutation

G12V

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-LAP-BUB1

Plasmid

#115903

Purpose

Used for expression of siRNA-resistant BUB1-WT from the FRT site of FlpIn cells upon addition of doxycyclin.

Depositor

Geert Kops

Article

Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

Insert

BUB1 (BUB1 Human)

Tags

EYFP

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-hygro-eGFP-STOP-HEC1

Plasmid

#114026

Purpose

Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-STOP-HEC1 (NDC80 Human)

Expression

Mammalian

Mutation

Start codon of Hec1 is mutated to stop codon

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv4.2LL

Plasmid

#114373

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv4.2LL, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv4.2LL (NEWENTRY )

Use

AAV

Tags

EYFP and Kv4.2LL

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C

Plasmid

#114375

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv2.1C, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv2.1C (NEWENTRY )

Use

AAV

Tags

EYFP and Kv2.1C

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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