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Plasmid#214354PurposeExpresses SlugABE-NNG and puromycin resistance genesDepositorInsertSlugABE-NNG and puromycin resistance genes
UseTagsExpressionMammalianMutationQ782R/S888R/L906R/N984S/E1012K/K1016IPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST1374-NLS-flag-linker-Cas9
Plasmid#44758DepositorInsertHuman codon optimized Cas9
UseCRISPRTags32 amino acid linker, Flag, and NLSExpressionMammalianMutationcodon-optimizedPromoterCMVAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
SlugCas9-HF
Plasmid#163796PurposeExpresses highly specific SlugCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationSlugCas9 (R247A, N415A, T421A, R656A)PromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AsCas12a-Plus-mCherry
Plasmid#191231PurposeTo express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificityDepositorInsertAsCas12a-Plus-P2A-mCherry
UseCRISPRTagsExpressionMutationE174R, R951K, R955APromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR
Plasmid#154003PurposeVector expressed CasRx and sgRNA_LACZ for AAV packageDepositorInsertCasRx
UseCRISPRTagsExpressionMutationPromoterEFSAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SlugABE-NNG
Plasmid#214356PurposeExpresses SlugABE-NNGDepositorInsertSlugABE-NNG
UseAAVTagsExpressionMammalianMutationQ782R/S888R/L906R/N984S/E1012K/K1016IPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SlugCBE-NNG-P2A-puro
Plasmid#214355PurposeExpresses SlugCBE-NNG and puromycin resistance genesDepositorInsertSlugCBE-NNG and puromycin resistance genes
UseTagsExpressionMammalianMutationQ782R/S888R/L906R/N984S/E1012K/K1016IPromoterAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST1374-N-NLS-flag-linker-Cas9-H840A
Plasmid#51129PurposeCas9 nickase optimized for nuclear import.DepositorInsertCas9 nickase
UseCRISPRTagsFlag and NLSExpressionMammalianMutationH840APromoterCMVAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PCS2-FlipGFP(Casp3 cleavage seq)
Plasmid#124432PurposeExpresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)DepositorInsertFlipGFP(Casp3 cleavage seq)
UseZebrafish (good expression)TagsExpressionMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP
Plasmid#172670PurposeFor expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition siteDepositorInsertSDHB pegRNA and SDHB_nick-sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-LbCas12a-Plus-mCherry
Plasmid#191230PurposeTo express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificityDepositorInsertLbCas12a-Plus-P2A-mCherry
UseCRISPRTagsExpressionMutationD156R, R883K, R887APromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry
Plasmid#124493PurposeExpresses FlipYFP (TEV cleavage sequence) and T2A mCherryDepositorInsertFlipYFP(TEV cleavage seq) T2A mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGL3-U6-ALDOB_pegRNA-Csy4RS-nick_sgRNA-EGFP
Plasmid#172668PurposeFor expression of transcript containing pegRNA and nick-sgRNA targeting human ALDOB gene from the U6 promoter with pegRNA flanked by Csy4 recognition siteDepositorInsertALDOB pegRNA and ALDOB_nick-sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP
Plasmid#172669PurposeFor expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition siteDepositorInsertRNF2 pegRNA and RNF2_nick-sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCG150_double_phiC31_attB
Plasmid#169693Purposecargo vector for the phiC31 system with, unc-119 rescue fragment, no fluorescent marker. Gateway R3R4 sites (for gene of interest insertion)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9HF1-P2A-puro
Plasmid#214357PurposeExpresses SpCas9HF1 and puromycin resistance genesDepositorInsertSpCas9HF1 and puromycin resistance genes
UseTagsExpressionMammalianMutationN497A/R661A/Q695A/Q926APromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-5U6-sgRNAs-hsyn-EGFP
Plasmid#112213PurposeTargeted DNA methylationDepositorInsertsgRNAs
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE4
Plasmid#181846PurposeFourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.DepositorInsertICUE4 (RAPGEF3 Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationGlutamine 122 in Epac1 domain mutated to glutamat…PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIα-ICUE4
Plasmid#181848PurposeICUE4 cAMP sensor tethered to the PKA RIα subunit.DepositorUseTagsECFP, PKA RIα, and cpVenus(L194)ExpressionMammalianMutationGlutamine 122 in Epac1 domain mutated to glutamat…PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry
Plasmid#181853PurposemCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.DepositorUseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-KMS
Plasmid#188038PurposePolycistronic human KLF4, MYC and SOX2 were cloned into the pMXs vectorDepositorUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PDE4D2cat-ICUE4
Plasmid#181847PurposeICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.DepositorUseTagsECFP, PDE4D2(86-418), and cpVenus(L194)ExpressionMammalianMutationGlutamine 122 in Epac1 domain mutated to glutamat…PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124428PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124434PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in DrosophilaDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST1374-N-NLS-flag-linker-Cas9-D10A
Plasmid#51130PurposeCas9 nickase optimized for nuclear import.DepositorInsertCas9 nickase
UseCRISPRTagsFlag and NLSExpressionMammalianMutationD10A (nickase mutant)PromoterCMVAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6-cj-E sgRNA
Plasmid#169915PurposeExpression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH
Plasmid#112211PurposeTargeted DNA methylationDepositorInsertC-terminus of dCas9
UseAAVTagsExpressionMutationD10APromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry
Plasmid#124429PurposeExpresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(TEV cleavage seq) T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124431PurposeExpresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafishDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseZebrafish (if expression level is too high in zeb…TagsExpressionMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
YW249_Pzipt17-loxP-H2BGFP-miniMOS
Plasmid#196063Purposea plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.DepositorInsertsH2B::GFP
NeoR
Minimal Mos1
zipt17p::zipt17::zipt17 3UTR
UseTagsExpressionWormMutationPromoterAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
YW347_Ptbb2UTR-loxP-RFP-miniMOS
Plasmid#196064Purposea plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.DepositorInsertsmyo2p driven tagRFP
NeoR
Minimal Mos1
UseTagsExpressionWormMutationPromotermyo-2Available sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-SARS2-B.1.617.2
Plasmid#172320Purposeexpressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.DepositorInsertSpike of B.1.617.2 strain (S SARS-CoV-2)
UseTagsExpressionMammalianMutationT19R, 156G, 157-158del, L452R, T478K, D614G, P681…PromoterCMV-FAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-SARS2-B.1.617.1
Plasmid#172319Purposeexpressing SARS-CoV-2 B.1.617.1 (kappa strain) spike protein for pseudovirus productionDepositorInsertSpike of B.1.617.1 strain (S SARS-CoV-2)
UseTagsExpressionMammalianMutationG142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…PromoterCMVAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-NTD-AVI
Plasmid#160475PurposeExpresses SARS-CoV-2 NTD domain with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-NTD
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-AVI
Plasmid#160476PurposeExpresses SARS-CoV-2 RBD domain with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_Mu
Plasmid#177578Purposeexpressing SARS-CoV-2 B.1.621 (Mu) spike protein for pseudovirus productionDepositorInsertSpike (Mu variant) (S SARS-CoV-2)
UseRetroviralTagsExpressionMammalianMutationT95I, Y144T, Y145S, R346K, E484K, N501Y, D614G, …PromoterCMVAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_Lambda
Plasmid#177579Purposeexpressing SARS-CoV-2 C.37 (lambda) spike protein for pseudovirus productionDepositorInsertSpike (Lambda variant) (S SARS-CoV-2)
UseRetroviralTagsExpressionMammalianMutation75-76VI, 246-253N, L452Q, F490S, D614G, T859N, la…PromoterCMVAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-AVI
Plasmid#160474PurposeExpresses SARS-CoV-2 S2P protein with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-SD1-AVI
Plasmid#160477PurposeExpresses SARS-CoV-2 RBD-SD1 domain with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD-SD1
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-mCherry-P2A-Cre-WPRE
Plasmid#107312PurposeEncoding hSyn-mCherry-CreDepositorHas ServiceAAV1InserthSyn-mCherry-P2A-Cre-WPRE
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_UCN1.0
Plasmid#208669PurposeExpresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in a cre-dependent mannerDepositorInsertGPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_SST1.0
Plasmid#208664PurposeExpresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in mammalian cellsDepositorInsertGPCR activation based somatostatin (SST) sensor GRAB_SST1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_NPY1.0
Plasmid#208675PurposeExpresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in mammalian cellsDepositorInsertGPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_CRF1.0
Plasmid#208655PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in mammalian cellsDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_SST1.0
Plasmid#208665PurposeExpresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in neuronsDepositorInsertGPCR activation based somatostatin (SST) sensor GRAB_SST1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_UCN1.0
Plasmid#208667PurposeExpresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in mammalian cellsDepositorInsertGPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4
Plasmid#107310PurposeEncoding dCas9-10xGCN4 driven by dSV40 promoterDepositorInsertdSV40-dCas9-10xGCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-VPR-S-L1-dCjCas9 MiniCAFE
Plasmid#169910PurposeExpression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.DepositorInsertMiniCAFE
UseCRISPRTagsExpressionMammalianMutationD8A, HNH-truncation (Δ495–609 aa) in CjCas9PromoterCMVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19_TELO2_dTAG
Plasmid#224380PurposeGenerates TELO2 dTAG N terminal knock-in cell linesDepositorInsertTELO2 (TELO2 Human)
UseTagsdTAGExpressionBacterialMutationSynonymous mutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NPY1.0
Plasmid#208676PurposeExpresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in neuronsDepositorInsertGPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only