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Plasmid#235274Purpose3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF5)-TS.FF5x1-bGH
Plasmid#235261PurposeComMAND closed-loop circuit regulating mRuby2 with miRE-FF5DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-tagBFP-bGH
Plasmid#235257PurposetagBFP expression to serve as a "filler" plasmid for transfectionsDepositorInserttagBFP
UseSynthetic BiologyTagsExpressionMammalianMutationSilent mutations to remove BsaI sites in tagBFPPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mGL(miRE.FF4)-bGH
Plasmid#235264PurposemicroRNA expression for dual-vector iFFL circuitDepositorInsertmGreenLantern
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EFS-mRuby2-bGH
Plasmid#235253PurposeComMAND base gene regulating mRuby2, EFS-drivenDepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEFS (human EF1a short, intronless)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2-bGH
Plasmid#235269PurposeComMAND base gene regulating mRuby2, EF1aDepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF6x1-mRuby2-bGH
Plasmid#235275Purpose3' microRNA target site expression for dual-vector iFFL circuit (open-loop)DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2-TS.FF4x1-bGH
Plasmid#235272Purpose3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2-TS.FF6x1-bGH
Plasmid#235273Purpose3' microRNA target site expression for dual-vector iFFL circuit (open-loop)DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Setd2CD-CatMut
Plasmid#235588PurposeDox-inducible expression of control catalytic-mutant Setd2 CD fused with scFVDepositorInsertSetd2 (Setd2 Mouse)
UseCRISPRTagsExpressionMutationR1599CPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Ring1bCD-CatMut
Plasmid#235583PurposeDox-inducible expression of control catalytic-mutant Ring1b CD fused with scFVDepositorInsertRing1b (Rnf2 Mouse)
UseCRISPRTagsExpressionMutationI53SPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-FLuc0_EMCV-Ren
Plasmid#210590PurposeExpresses SM(FLAG)-nonoptimal Firefly luciferase, and EMCVIRES-driven Renilla luciferaseDepositorInsertsSpaghetti monster (FLAG)-nonoptimal FLuc
Optimal Renilla luciferase
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterEMCV IRES and UBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-FLuc100_EMCV-Ren
Plasmid#210591PurposeExpresses SM(FLAG)-optimal Firefly luciferase, and EMCVIRES-driven Renilla luciferaseDepositorInsertsSpaghetti monster (FLAG)-optimal FLuc
Optimal Renilla luciferase
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterEMCV IRES and UBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_HD_160-223AA
Plasmid#221489PurposeNKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationDeletion of 1-159AA N-terminal and 224-371AA C-te…PromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
LacI pm
Plasmid#218097PurposePhagemid that expresses LacI but no phage proteins, used as a controlDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_CHO-Bip_gRNA1
Plasmid#183694PurposeCRISPRa gRNA expression vector for Chinese Hamster Bip (Hspa5)DepositorInsertgRNA targeting chinese hamster Bip (Hspa5)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRIN-E-shTAF12.364
Plasmid#105572PurposeDox-inducible mir30 MYB shRNA/dsRED expression with GFP marker and neo resistanceDepositorInsertTAF12 shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-MYB-TAD(251-327)
Plasmid#105619Purposebacterially express GST tagged MYB TADDepositorInsertMYB-TAD domain (Myb Mouse)
UseTagsGSTExpressionBacterialMutationamino acids 251-327PromoterAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-N3FLAG-TAF12-HFD(50-130)
Plasmid#105615Purposetransient express TAF12 HFD with 3*FLAG tag at N terminal in HEK 293T cellsDepositorInsertTAF12-HFD amino acid 50-130 with FLAG tag (Taf12 Mouse)
UseTags3*FLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
LENC-shTAF12.364
Plasmid#105579Purposeretrovirally express TAF12 shRNA with Neo resistance and mCherry markerDepositorInsertTAF12 shRNA #364
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only