We narrowed to 19,702 results for: CAL

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pLenti-eGFP-dSaCas9-blast

Plasmid

#191653

Purpose

Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

Insert

eGFP-T2A-dSaCas9

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX_305-N-dTAG_OGT

Plasmid

#155196

Purpose

N-terminal FKBP12(F36V)-2xHA tag on lentiviral OGT construct

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

2x HA and FKBP12(F36V)

Expression

Mammalian

Mutation

lacking N-terminal Met

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLP-PIGA2 (pLP300)

Plasmid

#168462

Purpose

Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA-P2A-mScarlet expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.

Depositor

Matthew Maurano

Article

Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

Type

Empty backbone

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Expression

Mammalian

Promoter

EF1a

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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iSH-iRFP670-sspB

Plasmid

#176102

Purpose

Heterodimerization with iLID, phosphatidylinositol 3-kinase derived iSH

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

phosphatidylinositol 3-kinase (Pik3r1 Mouse)

Expression

Mammalian

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-4mtD3cpv

Plasmid

#181874

Purpose

Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

Depositor

Hilmar Bading

Article

Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

Insert

4mtD3cpv

Use

AAV and Adenoviral

Expression

Mammalian

Mutation

the 4mtD3cpv CDS differs from the recorded GeneBa…

Promoter

CaMKIIa

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PTBP1-Halo

Plasmid

#175302

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PTBP1 (PTBP1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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mVenus-NES-SspB-MCS-P2A-iLIDcaax

Plasmid

#173864

Purpose

Mammalian expression of mVenus-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-MCS-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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AiP11466 - pAAV-eHGT_078m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1466)

Plasmid

#163508

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBetaGlobin

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-myosin Vb tail 1436-1846

Plasmid

#162714

Purpose

Mammalian expression of rat WT Myosin Vb with deletion of the 1435 N-terminal residues

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

Myosin Vb (Myo5b Rat)

Tags

GFP

Expression

Mammalian

Mutation

WT with deletion of the 1435 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-4580NES

Plasmid

#138376

Purpose

Booster-JNK, a Red-Shifted Genetically Encoded FRET Biosensor for JNK activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-JNK

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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HS38 pEYFPN1 anti-Homer1 nanobody

Plasmid

#135221

Purpose

Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody HS38 (Homer1 L. glama (llama))

Tags

6xHis-HA-EYFP

Expression

Mammalian

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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HS22 pEYFPN1 anti-Homer1 nanobody

Plasmid

#135219

Purpose

Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody HS22 (Homer1 L. glama (llama))

Tags

6xHis-HA-EYFP

Expression

Mammalian

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-A2AR

Plasmid

#106066

Purpose

Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-A2AR; Opto-A2AR; F8; 719)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-A2AR (ADORA2A Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RL3Sc

Plasmid

#109365

Purpose

Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)

Tags

1D4

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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CIAR_pcDNA5/FRT/TO

Plasmid

#86502

Purpose

Expresses CIAR in mammalian cells. Used to generate Flp-In stables.

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

CIAR (SOS1 Human)

Use

Frt

Expression

Mammalian

Mutation

T968L in SOScat

Promoter

CMV (tet operator)

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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Sema6b(L)-Fc-His

Plasmid

#72165

Purpose

Expresses the extracellular region of the Sema6B protein (entire extracellular domain; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6b (Sema6b Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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Dscaml1-Fc-His

Plasmid

#72072

Purpose

Expresses the extracellular region of the Dscaml1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscaml1 (Dscaml1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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L1cam-Fc-His

Plasmid

#72083

Purpose

Expresses the extracellular region of the L1CAM protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

L1cam (L1cam Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/SECFP-mATG5

Plasmid

#58744

Purpose

Expresses /SECFP-ATG5 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

autophagy related genes 5 (Atg5 Mouse)

Use

Retroviral

Tags

SECFP

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
PhyB-mCherry-MYO1

Plasmid

#51570

Purpose

Constitutively express myosin ring localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

MYO1 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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