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pCDH-EF1a-extGluc-mKate-NGFR

Plasmid

#104831

Purpose

extGluc luciferase for invivo Imaging, mKate and NGFR for flourescent detection

Depositor

Irmela Jeremias

Publication

Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

Inserts

mKate
NGFR

Use

Lentiviral

Expression

Mammalian

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pKK-mRuby3-TEV

Plasmid

#105780

Purpose

Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.

Depositor

Andrzej Dziembowski

Publication

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

mRuby3-TEV

Expression

Mammalian

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYFP-NEAT1m_v1-RT

Plasmid

#97089

Purpose

Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.

Depositor

Archa Fox

Publication

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

NEAT1m_v1-RT (NEAT1 Human)

Use

CRISPR

Tags

EYFP

Promoter

CMV

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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DHFR-PP7-VP64_T2A_GFP

Plasmid

#86167

Purpose

Expresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cells

Depositor

Amit Choudhary

Publication

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-PP7-VP64_T2A_GFP

Use

CRISPR and Lentiviral

Tags

Destabilized domain (N-terminus version) of E.col…

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1DA

Plasmid

#35028

Depositor

Sheila Stewart

Publication

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1DA (FEN1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

D181A--NUCLEASE DEAD POINT MUTANT

Promoter

CMV

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-PRKAR1B

Plasmid

#23376

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAR1B (PRKAR1B Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKG2

Plasmid

#23435

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKG2 (PRKG2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLifeact-mScarlet3-H (mYongHong)

Plasmid

#239415

Purpose

Expresses Lifeact-mScarlet3-H (aka mYongHong) in mammalian cells

Depositor

Zhifei Fu

Publication

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

Lifeact-mScarlet3-H

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-LATS2

Plasmid

#166474

Purpose

Expresses fusion of mEGFP and LATS2

Depositor

Shuguo Sun

Publication

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, LATS2 (LATS2 Human)

Expression

Mammalian

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3

Plasmid

#127128

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221-TCF7

Plasmid

#79498

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDS

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

TCF7 (TCF7 Human)

Use

Gateway Cloning

Promoter

no Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-Flp-DOG

Plasmid

#80084

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

Depositor

Connie Cepko

Publication

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

Flp-DOG

Expression

Mammalian

Promoter

CAG

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-TRE-DsRed-IRES-d24EGFP

Plasmid

#41945

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Publication

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-ULK3

Plasmid

#23646

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ULK3 (ULK3 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
CMV-miR-31 sponge

Plasmid

#25025

Depositor

Bob Weinberg

Publication

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

CMV-miR-31 sponge

Expression

Mammalian

Available since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBAD-hfYFP

Plasmid

#186522

Purpose

Hyperfolder YFP fluorescent protein: bacterial expression plasmid

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Tags

6xHis

Expression

Bacterial

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

araB

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::PuroR-bGHpA

Plasmid

#62439

Purpose

MXS_chaining vector with CMV::PuroR-bGHpA

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Puromycin with CMV Promoter

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-FLAG-CyclinF-ΔF

Plasmid

#32973

Depositor

Michele Pagano

Publication

D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

Insert

CYCLIN F (CCNF Human)

Tags

FLAG

Expression

Mammalian

Mutation

F-box mutations: L35A and P36A

Promoter

CMV

Available since

Feb. 3, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP4K2

Plasmid

#23644

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP4K2 (MAP4K2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLPC MYC hTel2

Plasmid

#22802

Depositor

Titia de Lange

Publication

Takai et al Cell. 2007 Dec 28. 131(7):1248-59.

Insert

Telomere Maintenance 2 (TELO2 Human)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles