166,461 results

1. Lenti-4XPRE-GFP

   Plasmid

   #206170

   Purpose

   pMVP Gateway destination vector (3rd generation lentiviral vector) containing 4 consensus PGR binding sites upstream of a minimal 2YBTATA promoter

   Depositor

   Renee van Amerongen

   Article

   Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

   Insert

   4X PRE-eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   2YBTATA

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

   Plasmid

   #138148

   Purpose

   Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NES

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. ABE8e-Cas9NG

   Plasmid

   #203328

   Purpose

   Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   ABE8e-Cas9NG
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1hSIRT3_H248Y_HA

   Plasmid

   #24492

   Depositor

   Eric Verdin

   Article

   Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

   Insert

   Human Sirtuin 3 catalytically inactive mutant (SIRT3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H248Y

   Promoter

   Available Since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. p177bp-LacOx162-Hyg

   Plasmid

   #210215

   Purpose

   Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Insert

   162xLacO repeats derived from: PMID: 12864855.
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ166-OsPE2

   Plasmid

   #141080

   Purpose

   Gateway entry clone for CRISPR-Cas9(H840A) prime editing

   Depositor

   Yiping Qi

   Article

   Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

   Insert

   zCas9(H840A)-OsM-MLV-RT
   

   Use

   CRISPR; Gateway compatible zcas9(h840a)-osm-mlv-rt

   Tags

   3x FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221 msNLRP3

   Plasmid

   #218639

   Purpose

   Gateway shuttling vector for cloning C-terminal NLRP3 tags (contains no stop codon).

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (Nlrp3 Mouse)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Thy1.1-G5

   Plasmid

   #234839

   Purpose

   pMP71 backbone. Expresses Thy1.1 with N-terminal 5xGly (G5)

   Depositor

   Gabriel Victora

   Article

   Nakandakari-Higa et al Nature. 2024 Mar;627(8003):399-406. doi:

   Insert

   Thy1.1 (Thy1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP-P2A-5xGly

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRL_SRSF2_WT_mCherry

   Plasmid

   #84020

   Purpose

   expression of SRSF2 in mammalian cells

   Depositor

   Robert Bradley

   Article

   Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

   Insert

   SRSF2 (SRSF2 Human)
   

   Use

   Lentiviral

   Tags

   2TA-mCherry and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-AAV-syn-jGCaMP8m-WPRE

    Plasmid

    #162375

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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