We narrowed to 29,532 results for: MUT

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pENTR_L1-Turbo-NES-YFP-STOP-L2

Plasmid

#127358

Purpose

gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, NES, V5, and YFP

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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FKBP-CD28TMD-NTEVp(H75E)

Plasmid

#137829

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(H75E)

Tags

3x FLAG

Expression

Mammalian

Mutation

Mutated Histidine 75 to Glutamic Acid in NTEVp fr…

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Ras85D CA

Plasmid

#52247

Purpose

Drosophila expression of YFP-Ras85D constitutively active mutant

Depositor

Matthew Scott

Insert

Ras85D (Ras85D Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q61L; CA mutation

Promoter

GAL4

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA

Plasmid

#100279

Purpose

In vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutant

Depositor

Yi Zhang

Article

Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

Insert

Kdm6b (Kdm6b Mouse)

Tags

Flag epitope tag

Expression

Mammalian

Mutation

Mutated catalytic domain (1025aa-End) (please see…

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pFUGW-GtACR1_C102A-EYFP

Plasmid

#119196

Purpose

Step-function mutant of Guillardia theta anion channelrhodopsin 1 (GtACR1) with dramatically prolonged open state

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin GtACR1 step-function mutant C102A

Use

Lentiviral

Tags

EYFP

Mutation

changed cysteine 102 to alanine

Promoter

Ubiquitin

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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PTPRA-SHC

Plasmid

#139611

Purpose

StrepIII-HA-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

PTPRA (PTPRA Human)

Tags

StrepIII-HA

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-G2-ATP8-V5 (puromycin)

Plasmid

#86852

Purpose

mammalian expression of ATP8 with G2 mitochondrial targeting sequence and V5 tag

Depositor

Matthew O'Connor

Article

Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

Insert

ATP8 (ATP8 Human)

Tags

ATP5G2 (G2) mitochondrial targeting signal and V5

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_A321V

Plasmid

#98671

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with A321V

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Mutation

A321V

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMGIB-3xFLAG-pontin

Plasmid

#53608

Purpose

Retroviral expression of human pontin

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

pontin (RUVBL1 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

pontin CDS mutated to confer resistance to shRNA.…

Promoter

LTR

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pET28a-PKM2-H391Y

Plasmid

#122692

Purpose

Express His-tagged PKM2 (H391Y) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Mutation

H391Y

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR-L26A,F27A

Plasmid

#89084

Purpose

mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

hAR FxxLF mutant (AR Human)

Expression

Mammalian

Mutation

hAR-L26A,F27A

Promoter

CMV

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
pLenti-SPA2G231V

Plasmid

#42079

Depositor

Christine Garcia

Article

Maitra et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

Surfactant Protein -A2 (SFTPA2 Human)

Use

Lentiviral; Plenti

Tags

V5

Expression

Bacterial and Mammalian

Mutation

Changed Glycine 231 to Valine

Promoter

CMV

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
mEmerald-Caveolin-N-10

Plasmid

#54026

Purpose

Localization: Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

Insert

Caveolin (CAV1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
GFP-nArgBP2ΔNSE

Plasmid

#74515

Purpose

Express GFP-nArgBP2ΔNSE fusion protein in mammalian cells. nArgBP2ΔNSE is the mutant without the neuronal specific exon of nArgBP2.

Depositor

Guoping Feng

Article

Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

Insert

nArgBP2delNSE (Sorbs2 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe puro H-Ras G12V T35S

Plasmid

#12274

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V T35S (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The T35S mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-hAR-F23A

Plasmid

#89089

Purpose

mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

hAR-FxxLF mutant (AR Human)

Expression

Mammalian

Mutation

hAR-F23A

Promoter

CMV

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
pHHM.X513-iNicSnFR3b-(V7)

Plasmid

#124881

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR3b-(V7)

Tags

HA Tag, His Tag, and NA

Expression

Bacterial

Mutation

NA

Promoter

T7

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Neo-PuroDTK

Plasmid

#84036

Purpose

PiggyBac cassette contains Neomycin, puromycin and TK genes

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Expression

Bacterial and Mammalian

Promoter

CAG promoter

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_mup53_Ex5

Plasmid

#85535

Purpose

Inducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

mu p53

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pPong_v2

Plasmid

#47101

Purpose

Expresses the Pong ORF1 and transposase from rice to allow mPing movement. Contains ORF1 and transposase driven by a native soybean promoter. Contains mPing and BAR for basta selection.

Depositor

Wayne Parrott

Article

Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d

Inserts

mPing
Pong Open Reading Frame 1 (ORF1)
Pong Transposase

Use

Plant expression

Promoter

GmUbi_AP, GmUbi_XS, and no promoter

Available Since

Oct. 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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