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pcDNA5-FRT-TO-Nsp5

Plasmid

#157685

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp5 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp1

Plasmid

#157679

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp1 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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evoCas9

Plasmid

#138558

Purpose

Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

evoCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

M495V, Y515N, K526E, R661Q

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBBA57-TEV-His12

Plasmid

#137033

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66270.1 (BB_A57 Borrelia burgdorferi B31)

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length BBA57, contains signal pep…

Promoter

T7

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CreLite

Plasmid

#131785

Purpose

AAV donor/transfer vector expressing CreLite system components, PhyBΔCreC and PIF6CreN, driven by CBh promoter

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC-P2A-PIF6CreN

Use

AAV, Cre/Lox, and Synthetic Biology

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSK267

Plasmid

#129272

Purpose

"ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

NLS-VP16-ZF 43-8-NES

Tags

6xHA

Expression

Yeast

Promoter

SUP35

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_SRY_P2A_Hygro_Barcode

Plasmid

#120485

Purpose

Barcoded lentiviral vector to express SRY in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SRY (SRY Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_POU5F1_P2A_Hygro_Barcode

Plasmid

#120474

Purpose

Barcoded lentiviral vector to express POU5F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

POU5F1 (POU5F1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_HOXA10_P2A_Hygro_Barcode

Plasmid

#120452

Purpose

Barcoded lentiviral vector to express HOXA10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXA10 (HOXA10 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ133C2.0

Plasmid

#99893

Purpose

Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL003877-Cas9

Plasmid

#100581

Purpose

The promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

Inserts

AAEL003877-hspcas9-GFP
Opie-dsRed

Use

CRISPR

Tags

GFP and dsRED

Expression

Insect

Promoter

AAEL003877 and Opie2

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDQM048

Plasmid

#247634

Purpose

to generate mRNA for membrane mStayGold(Ando) for zebrafish

Depositor

Gokul Upadhyayula

Article

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mStayGold(A)

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pDQM049

Plasmid

#247635

Purpose

to generate mRNA for membrane mStayGold(E138D) for zebrafish

Depositor

Gokul Upadhyayula

Article

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mStayGold(E138D)

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pZZMP122

Plasmid

#231756

Purpose

Mammalian expression of SPO11-YF

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

SPO11-YF (Spo11 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Y138F

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pZZMP154

Plasmid

#231760

Purpose

Mammalian expression of LZ-SPO11

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

LZ-SPO11 (Spo11 Mouse, Budding Yeast)

Tags

Flag-LeucineZipper

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Tom70NTD-ABHD16Acyto-mNG

Plasmid

#195159

Purpose

mammalian expression of outer mitochondrial membrane localized ABHD16A tagged with mNeonGreen

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

Tom70NTD (TOMM70 Human)

Tags

ABHD16A cytoplasmic domain and mNeonGreen

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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p8209 MSCV-neo C-V5 Empty

Plasmid

#193975

Purpose

Empty vector control

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Type

Empty backbone

Use

Retroviral

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:miRFP670-cetn4

Plasmid

#192357

Purpose

zfCETN4 tagged with miRFP670, expressed under the UAS promoter

Depositor

Darren Gilmour

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

CETN4 (cetn4 Zebrafish)

Tags

miRFP670

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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T_antiEGFPsynsrRNA_UA

Plasmid

#185974

Purpose

Expresses antiEGFPsynsrRNA from PlTetO-1 promoter in E.coli Dh5alpha cell. For experiment use Dh5alphaz1.

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biosens Bioelectron. 2020 Nov 1;167:1124

Insert

PlTetO-1 AntiEGFPsynsrRNA

Use

Synthetic Biology

Promoter

PlTetO-1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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L_EGFP_AC

Plasmid

#185971

Purpose

Expresses EGFP from PlLaco-1 promoter in E.coli Dh5alpha cell. For experiment use Dh5alphaz1

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biosens Bioelectron. 2020 Nov 1;167:1124

Insert

Pl-LacO1-Egfp

Use

Synthetic Biology

Promoter

PlLacO-1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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