We narrowed to 7,679 results for: URE
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Plasmid#189950PurposeLentiviral vector to co-express a mouse Itgb1 spsgRNA (sg1-Itgb1) with NLS-mScarlet-IDepositorInsertNLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #1
UseLentiviralExpressionMammalianAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pW402-lenti-sg2-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#189951PurposeLentiviral vector to co-express a mouse Itgb1 spsgRNA (sg2-Itgb1) with NLS-mScarlet-IDepositorInsertNLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #2
UseLentiviralExpressionMammalianAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pW403-lenti-sg3-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#189952PurposeLentiviral vector to co-express a mouse Itgb1 spsgRNA (sg3-Itgb1) with NLS-mScarlet-IDepositorInsertNLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #3
UseLentiviralExpressionMammalianAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATX3-D1
Plasmid#184246PurposeRecombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertAtaxin-3 Josephin domain and UIM1 (ATXN3 Human)
Tags6x His-Tag and TEV cleavage siteExpressionBacterialMutationPremature stop codon N263XPromoterT7 promoterAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAP14
Plasmid#186262PurposewgaA (a.k.a expA1) and wgaB (a.k.a expA23), bicistronic, under light light responsive PEL222 promoter and EL222 under constitutively active LacIq promoterDepositorInsertsExpressionBacterialPromoterLacIq (CGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCC…Available SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1043
Plasmid#169621PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA) .DepositorInsertPCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH266-Tier1-OTetO7-PCMVmin2-SEAP-sTRSV
Plasmid#169589PurposeTier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR sTRSV ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRsTRSV-pA).DepositorInserttetracycline-controlled SEAP expression
ExpressionMammalianPromoterTetO7-PCMVmin-2Available SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1042
Plasmid#169620PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH267-Tier1-OTetO7-PCMVmin2-SEAP-env140
Plasmid#169590PurposeTier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR env140 ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRenv140-pA).DepositorInserttetracycline-controlled SEAP expression
ExpressionMammalianPromoterTetO7-PCMVmin-2Available SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2304-Tier1-Tactb-PPGK-NLuc_TB
Plasmid#169549PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertTactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
ExpressionMammalianPromoterPPGKAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR
Plasmid#169499PurposeTier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)DepositorInsertPRPBSA-driven YPet-P2aA-PuroR
ExpressionMammalianPromoterPRBSAAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1047-Tier1-PhCMV-YPet-p2A-PuroR
Plasmid#169498PurposeTier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).DepositorInsertPCMV-driven YPet-P2aA-PuroR
ExpressionMammalianPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso2-6xmut_U
Plasmid#147732PurposeMammalian Expression of HsNot1iso2-6xmutDepositorInsertHsNot1iso2-6xmut (CNOT1 Human)
ExpressionMammalianMutation4 silent and two non silent G917E and R2353Q muta…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]
Plasmid#149427PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.DepositorInsertsU6.3-gRNAs[TraB, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10
UseCRISPRTagseGFPExpressionInsectAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot2-del458-482-dsRNAres_U
Plasmid#147716PurposeInsect Expression of DmNot2-del458-482-dsRNAresDepositorInsertDmNot2-del458-482-dsRNAres (Rga Fly)
ExpressionInsectAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-2R-2K+3D
Plasmid#178680PurposeBacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues removed, two Lys removed, three Asp added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-2R+2K+3D (HNRNPA1 Human)
Tags6xHisExpressionBacterialMutationtwo Arg residues removed, two Lys added, three As…Available SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-1 (BTLA ITIM)-mGFP
Plasmid#180817PurposeExpression of human PD-1 with immunoreceptor tyrosine-based inhibitory motif (ITIM) from BTLA, fused to mEGFPDepositorInsertPD-1 (BTLA ITIM) (PDCD1 Human)
UseLentiviralTagsmEGFPMutationreplaced PD-1-ITIM (VDYGEL) with BTLA-ITIM (IVYAS…PromoterSFFVAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4G_601-660-GB1_AD
Plasmid#148506PurposeBacterial Expression of DmeIF4G_601-660DepositorInsertDmeIF4G_601-660 (eIF4G1 Fly)
ExpressionBacterialAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-4R-2K+5D
Plasmid#178681PurposeBacterial expression of N-terminally 6His tagged A1-LCD with four Arg residues removed, two Lys removed, five Asp added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-4R+2K+5D (HNRNPA1 Human)
Tags6xHisExpressionBacterialMutationfour Arg residues removed, two Lys added, five As…Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVD1 tRNA-gRNA E4-En-1 (GB2243)
Plasmid#160565PurposetRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.DepositorInserttRNA-gRNA position E4-En-1 (Multiplexing Edit)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only