We narrowed to 166,390 results for: addgene

1. qTAG-AAVS1-PGK-Puro-moxGFP

   Plasmid

   #227275

   Purpose

   AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX335-EN477

   Plasmid

   #86232

   Purpose

   For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   spCas9-nickase and sgRNA against mouse CTCF STOP Codon
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CMV-cGAS-N-HA

   Plasmid

   #130913

   Purpose

   Expresses human cGAS N terminus(aa1-159)-HA; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   cGAS N terminus
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. IFNAR2_pCSdest

   Plasmid

   #53914

   Purpose

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   IFNAR2 (IFNAR2 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

5. Lenti-LbCpf1-Blast

   Plasmid

   #84751

   Purpose

   Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   LbCpf1
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-3xHA (C terminal on insert)

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVTHM-shN

   Plasmid

   #83046

   Purpose

   Control shRNA pLVTHM-shN

   Depositor

   Joe Landry

   Article

   Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

   Insert

   shRNA Neg Control
   

   Use

   Lentiviral

   Promoter

   TRE-H1

   Available Since

   Oct. 24, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pLKO mouse shRNA 1 raptor

   Plasmid

   #21339

   Depositor

   David Sabatini

   Article

   Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

   Insert

   raptor shRNA 1 (Rptor Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 7, 2009

   Availability

   Academic Institutions and Nonprofits only

8. LiPOP1

   Plasmid

   #168264

   Purpose

   A single-component optogenetic tool for photoswitchable necroptosis

   Depositor

   Yubin Zhou

   Article

   He et al Adv Sci (Weinh). 2021 May 6;8(13):210042

   Insert

   MLKL(1-125) (MLKL Human)
   

   Tags

   mCherry, CRY2-PHR

   Expression

   Mammalian

   Mutation

   H15A, K16A, R17A

   Promoter

   CMV

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1 p18 shRNA

   Plasmid

   #26631

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   p18 shRNA (LAMTOR1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pKSB-AeU6-3gRNA

    Plasmid

    #183914

    Purpose

    gRNA-expressing plasmid under Aedres aegypti AAEL017763 U6 promoter

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    gRNA scaffold for protospacer cloning into BbsI sites
    

    Promoter

    Aedes aegypti U6 promoter (AAEL017763)

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. mMaple-OMP25

    Plasmid

    #141151

    Purpose

    Photoconvertible fluorescent protein targeted to the outer mitochondrial membrane (OMM) in mammalian cells.

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    OMP25 (Synj2bp Rat)
    

    Tags

    mMaple

    Expression

    Mammalian

    Mutation

    OMP25 c terminus from addgene plasmid #69598

    Promoter

    CMV

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. sh-RNA hAtg13

    Plasmid

    #31971

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    Atg13 (ATG13 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pBabe-puro GSK3 beta (S9A)

    Plasmid

    #14128

    Depositor

    Brendan Manning

    Article

    Zhang et al Mol Cell. 2006 Oct 20. 24(2):185-97.

    Insert

    GSK3 beta (S9A) (GSK3B Human)
    

    Use

    Retroviral

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    S9A. Mutant version of GSK3 that lacks the inhibi…

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pSLIK TT 3xFLAG SMAD4 neo

    Plasmid

    #83273

    Purpose

    Lentiviral vector for inducible expression of SMAD4 with 3xFLAG N-terminal tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    SMAD4 (SMAD4 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

15. pCMV-T7-AcrIIA16_Lmo-NLS(SV40) (KAC508)

    Plasmid

    #134332

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Lmo with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA16_Lmo
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-ms-Sox7

    Plasmid

    #50780

    Purpose

    retroviral expression of mouse Sox7

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

    Insert

    Sox7 (Sox7 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas9-EcRT-GAL-HIS3

    Plasmid

    #232093

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas9-EcRT-GAL-Nat

    Plasmid

    #232090

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9-EcRT-GAL-Kan

    Plasmid

    #232092

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. OA-1053B

    Plasmid

    #184007

    Purpose

    Expresses arrays of gRNA targeting hh promoter under U6b/c promoter

    Depositor

    Omar Akbari

    Article

    Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

    Insert

    U6b:sgRNAhh1 - U6c:sgRNAhh2
    

    Expression

    Insect

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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