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We narrowed to 10,082 results for: crispr plasmids

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Showing: 7081 - 7100 of 10082 results

  1. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid
    #117687
    Purpose
    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.
    Depositor
    Erik Sontheimer
    Article
    Gao et al Methods Enzymol. 2019;616:365-383. doi:
    Inserts
    Kanamycin cassette
    TetR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and hPGK
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    117687-plasmid-map-sequence-id-228342

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  5. eSpCas9(1.1)-Puro

    Plasmid
    #117686
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Depositor
    Luc Leyns
    Article
    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    PuroR (R166H)
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    U6
    Available Since
    Feb. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    117686-plasmid-map-sequence-id-236350

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  6. pJE53

    Plasmid
    #194153
    Purpose
    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.
    Depositor
    Edward Geisinger
    Article
    Bai et al J Bacteriol. 2021 May 20;203(12):e005652
    Insert
    sgRNA with mrfp control guide sequence
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    194153-plasmid-map-sequence-id-382435

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  11. pV92.2

    Plasmid
    #78409
    Purpose
    pKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array driven by its native leader, and the tmRNA::td intron construct
    Depositor
    Andrew Fire
    Article
    Silas et al Science. 2016 Feb 26;351(6276):aad4234.
    Inserts
    RT-Cas1
    Cas2
    Available Since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    78409-plasmid-map-sequence-id-146291

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  13. pYDE007

    Plasmid
    #194152
    Purpose
    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, CarbR. Contains mrfp nontargeting control guide sequence.
    Depositor
    Edward Geisinger
    Article
    Bai et al J Bacteriol. 2021 May 20;203(12):e005652
    Insert
    sgRNA with mrfp control guide sequence
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    194152-plasmid-map-sequence-id-382443

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  15. pQdCas12a.luxR(mut)-empty

    Plasmid
    #236037
    Purpose
    The plasmid pQdCas12a.luxR(mut)-empty expresses the dCas12a endonuclease. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI, dcas12a, and lacZ-alpha (fragment).
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236037-plasmid-map-sequence-id-469909

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  20. dCas9-NLS-gs10-cGR2176

    Plasmid
    #188258
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cGR2176
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    188258-plasmid-map-sequence-id-377988

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Showing: 7081 - 7100 of 10082 results