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pMito.iGABASnFR.F102Y.Y137L

Plasmid

#112177

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Expression

Mammalian

Mutation

F102Y.Y137L

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGL4.26_4xUAS_DSCP_GFP

Plasmid

#111931

Purpose

Vector to express GFP (under the control of the DSCP and an array of 4 UAS sites)

Depositor

Alexander Stark

Article

Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

Insert

4xUAS GFP

Expression

Insect

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pPUR-hU6-sgRNA-Sirius-8XMS2

Plasmid

#121942

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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EC70188

Plasmid

#209449

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

HvU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSmGFP

Plasmid

#186542

Purpose

LSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmid

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

6xHis

Expression

Bacterial

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

araB

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Crop-Seq-BFP-WPRE-TS-hU6-BsmbI

Plasmid

#137993

Purpose

Backbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencing

Depositor

Amy Brock

Article

Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPAmCherry1-C1

Plasmid

#31929

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Expression

Mammalian

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-Tet1-CD

Plasmid

#83341

Purpose

To introduce MS2 coat protein fused Tet1-CD

Depositor

Ronggui Hu

Article

Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

Insert

MS2-Tet1-CD

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-R-Cdc42

Plasmid

#180578

Purpose

ddRFP sensor of Cdc42

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Cdc42

Expression

Mammalian

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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ABE8.20-NG

Plasmid

#226857

Purpose

Expresses ABE8.20-NG base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

ABE8.20-NG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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LifeAct-LSSmGFP

Plasmid

#186534

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

LifeAct

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-CytERM-mCherry-(F30-2xPepper)10

Plasmid

#129403

Purpose

CytERM-mCherry mRNA reporter tagged with (F30-2xPepper)10

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

CytERM-mCherry-(F30-2xPepper)10

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-LTB

Plasmid

#184605

Purpose

For expression of a fluorescent sensor for leukotriene B4 in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-LTB

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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FosB

Plasmid

#187908

Purpose

Expresses FosB in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

FosB (Fosb Mouse)

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDIRE

Plasmid

#26745

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Insert

iCre expression cassette

Use

Cre/Lox; Flp/frt

Expression

Mammalian

Available Since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pAG1169 FIREmate-ECFP-Cb5

Plasmid

#205167

Purpose

Expresses FIREmate-ECFP-Cb5 in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

FIREmate-ECFP-Cb5

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMSP1E1D1

Plasmid

#161605

Purpose

Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E1D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

Plasmid

#175540

Purpose

Expression of HaloTag9 in the endoplasmic reticulum of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CalR-HaloTag9-KDEL

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Leu2 2u

Plasmid

#220622

Purpose

empty backbone for shuttle vector construction

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Insert

empty vector (LEU2 Budding Yeast)

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAG1186 Lyn11-ECFP-FIREmate

Plasmid

#205168

Purpose

Expresses Lyn11-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

Lyn11-ECFP-FIREmate

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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