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Tad-CBE(TCT1)

Plasmid

#235065

Purpose

Expresses Tad-CBE(TCT1) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(TCT1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_HDAC3

Plasmid

#183299

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC3

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_DNMT1

Plasmid

#183282

Purpose

All-in-One CRISPRko system with a guide RNA that targets DNMT1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

DNMT1

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pClathrin-miRFP670nano

Plasmid

#127430

Purpose

Clathrin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

miRFP670nano-clathrin light chain

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_013

Plasmid

#123676

Purpose

Encodes ConL5 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L5-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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GLURP-bio-His

Plasmid

#50802

Purpose

Expresses enzymatically monobiotinylated full length GLURP

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

GLURP

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pC0044-EF1a-RanCas13b-NES-mapk

Plasmid

#103855

Purpose

Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

RanCas13b

Use

CRISPR and Lentiviral

Tags

3xHA and mapk NES

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(ACC3)

Plasmid

#235062

Purpose

Expresses Tad-CBE(ACC3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACC3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2_hMGFP-60A

Plasmid

#200393

Purpose

A plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

monster GFP

Expression

Mammalian

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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myc-GABAAR Gamma2

Plasmid

#127619

Purpose

N-terminal myc-tagged Gamma2 subunit of GABAAR

Depositor

Josef Kittler

Article

Arancibia-Carcamo et al Proc Natl Acad Sci U S A. 2009 Oct 13;10

Insert

GABA-A receptor subunit gamma-2

Tags

myc

Expression

Mammalian

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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ITPKA_3xmNeonGreen

Plasmid

#129606

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

F-tractin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

Plasmid

#174529

Purpose

Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

Depositor

Yu-Hsuan Tsai

Article

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Inserts

PylS
PylT

Tags

Flag

Expression

Mammalian

Promoter

U6

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-B1

Plasmid

#31705

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-106a

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
SERA9-bio-His

Plasmid

#50820

Purpose

Expresses enzymatically monobiotinylated full length SERA9

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA9

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 12, 2014

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P19

Plasmid

#124789

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry-mNeonGreen

Use

Pcr template

Tags

mCherry-mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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CT452

Plasmid

#233665

Purpose

Red light-inducible Flp-N; Flp-N396-FHY1

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Flp-N396-FHY1

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT438

Plasmid

#233666

Purpose

Red light-inducible Flp-N; Flp-N27-PhyA

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Flp-N27-PhyA

Expression

Mammalian

Mutation

None

Promoter

CAG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT445

Plasmid

#233668

Purpose

Red light-inducible Flp-C; Flp-397C-PhyA

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Flp-397C-PhyA

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT447

Plasmid

#233663

Purpose

Red light-inducible Flp-C; Flp-28C-FHY1

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Flp-28C-FHY1

Expression

Mammalian

Mutation

None

Promoter

CAG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pNkx-GFP

Plasmid

#45497

Depositor

Sean Wu

Article

Wu et al Cell. 2006 Dec 15;127(6):1137-50. Epub 2

Insert

2.5kb mouse Nkx2.5 enhancer and promoter fragment (Nkx2-5 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

2.5kb Nkx2.5 enhancer and promoter fragment

Available Since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

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