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A*24:07:01

Plasmid

#203283

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*24:07:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-PGK-hKLF4

Plasmid

#60435

Purpose

Over-expression of human KLF4

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Insert

KLF4 (KLF4 Human)

Expression

Mammalian

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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A*33:01:01

Plasmid

#203288

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*33:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pICSL11055

Plasmid

#68252

Purpose

Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos

Use

Synthetic Biology

Expression

Plant

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT(UUA)_FLAG-AcKRS

Plasmid

#140010

Purpose

Plasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

AcKRS-3 (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Mutation

L301M, L305I, Y306F, L309A, C348F

Promoter

EF1

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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A*03:02:01

Plasmid

#203294

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*03:02:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA Ago2

Plasmid

#10821

Depositor

Thomas Tuschl

Article

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

Argonaute 2 (AGO2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

R668H. Additionally, N terminus different from wi…

Available Since

Oct. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-PAFAH1B1_R22-

Plasmid

#92431

Purpose

mammalian expression of PAFAH1B1 R22 mutant. The protein is truncated and non functional (negative control).

Depositor

Ralf Brandes, Matthias Leisegang

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Expression

Mammalian

Mutation

R22 (stop codon in position 22)

Promoter

CMV

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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attB53_SNCB+_attB53

Plasmid

#183614

Purpose

RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Inserts

LaG17 GFP nanobody SynNotch tTA
TRE-mCherry-SV40pA
tagBFP

Use

Rmce

Tags

Myc and human CD8a signal sequence

Expression

Mammalian

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pPlacPbuCas13b-gRNA-T-MS2

Plasmid

#184839

Purpose

Expression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119 and lac promoter

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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