165,185 results

1. PVALB mAb _BCN14.1.1C9_HC_Mouse IgG2a

   Plasmid

   #182888

   Purpose

   Mammalian expression of PVALB mouse mAb HC gene

   Depositor

   Heng Zhu

   Insert

   anti-PVALB (human) heavy chain (PVALB Mouse)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   No

   Promoter

   CMV

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pE5770

   Plasmid

   #170777

   Purpose

   expression of hybrid proteins in E. coli

   Depositor

   Anthony Schryvers

   Article

   Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

   Insert

   Kan resistance (kanR )
   

   Use

   Tags

   polyhisbioMbpTEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC57-sgRNA-MS2-U6

   Plasmid

   #171694

   Purpose

   To construct expression vector for tBE-V5-mA3

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   sgRNA scaffold-MS2-U6
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-ccdB-boxB-tBE-V5-mA3

   Plasmid

   #171693

   Purpose

   Expresses tBE-V5-mA3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   ccdB-sgRNA scaffold-boxB-tBE-V5-mA3
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1a

   Available since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSG5-HA-p300

   Plasmid

   #89094

   Purpose

   mammalian expression of full-length human p300 in pSG5

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

   Insert

   p300 (EP300 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-APEX2-C Cloning Vector

   Plasmid

   #128146

   Purpose

   To express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEM

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   Flag-APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLL3.7-TNFa-shRNA3

   Plasmid

   #72598

   Purpose

   Express shRNA against mouse TNF-alpha

   Depositor

   Kazuhiro Oka

   Article

   Ogawa et al PLoS One. 2014 Mar 18;9(3):e92073. doi:

   Insert

   mouse TNF-alpha shRNA (Tnf Mouse)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Jan. 11, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. SARS-CoV-2 S HexaPro

   Plasmid

   #154754

   Purpose

   Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant

   Depositor

   Jason McLellan

   Article

   Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505

   Insert

   Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

   Expression

   Mammalian

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Promoter

   Available since

   July 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pJ3H-Mst1

   Plasmid

   #12203

   Depositor

   Jonathan Chernoff

   Article

   Creasy et al J Biol Chem. 1996 Aug 30. 271(35):21049-

   Insert

   Mst1 (STK4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQCXIZ GFP PODXL B WT

    Plasmid

    #202414

    Purpose

    Expression of GFP-tagged PODXL (isoform B) WT

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. T7-TPase

    Plasmid

    #51818

    Purpose

    For in vitro transcription of Tol2 transposase using T7 Promoter

    Depositor

    Elly Tanaka

    Article

    Khattak et al Nat Protoc. 2014 Mar;9(3):529-40. doi: 1

    Insert

    Tol2 Transposase
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEXTK-Pak1 70-117

    Plasmid

    #12217

    Depositor

    Jonathan Chernoff

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    PBD (p21-binding domain), aa 70-117

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRUPATH Triple GaoA

    Plasmid

    #196051

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

    Plasmid

    #50461

    Purpose

    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    hM4D(Gi)-mCherry (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA and mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    EF1a

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. JDS246

    Plasmid

    #43861

    Purpose

    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters

    Depositor

    Keith Joung

    Insert

    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    

    Use

    CRISPR

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85006

    Purpose

    For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    EGFP-ires-tTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_Floxed-STOP mCherry

    Plasmid

    #122963

    Purpose

    mCherry-based fluorescent reporter for Cre-loxP DNA recombination reaction

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

    Insert

    pcDNA3.1_Floxed-STOP mCherry
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV-LMP2A-IRES-mCherry

    Plasmid

    #220353

    Purpose

    Expresses EBV latent gene LMP2A in mammalian cells

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    LMP2A (LMP-2A Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)

    Plasmid

    #86419

    Purpose

    C-terminal PCR tagging module for yeast codon optimized split GFP1-10

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. MaLionR

    Plasmid

    #113908

    Purpose

    Red ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    MaLionR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge