We narrowed to 7,206 results for: SIM
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Plasmid#221717PurposeExpression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.DepositorInserthsN-drAgo2 (ago2 Zebrafish)
UseFish expressionTags3xFLAGMutationSwap of N-domain for human Ago2 N-domainPromotersimian CMV IE94Available SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2EF-DY
Plasmid#221723PurposeExpression of human Ago2 with E637D and F666Y mutations and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2EF-DY (AGO2 Human)
UseFish expressionTags3xFLAGMutationE637D and F666Y in active sitePromotersimian CMV IE94Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 Ta-sro1 WWE-PARP
Plasmid#192548PurposepENTR plasmid Triticum aestivum sro1, amino acids 1-434, cultivar SR3, no stop codonDepositorInsertSIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
UseGateway entry vectorAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINF Ta-sro1 PARP
Plasmid#192549PurposeE. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)DepositorInsertSIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)
TagsHis6, 3C protease cleavage siteExpressionBacterial, Insect, and Mamm…PromoterT7 promoterAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG
Plasmid#154778Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertLaG17-SynNotch
TagsLaG17ExpressionMammalianMutation204TAA 442TAG in LaG17-SynNotch, hybrid PylT with…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-Bcan-Ntrk1_4
Plasmid#136413PurposeLentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.DepositorInsertU6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-Myb-Qk_1
Plasmid#136415PurposeLentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.DepositorInsertU6_sgRNA(Myb)_U6_sgRNA(Qk)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-SOCS1-Y80D
Plasmid#129516PurposeRetroviral vector for the expression of human SOCS1 with a Y80D mutation and a FLAG tag in N-terminalDepositorInsertSOCS1 (SOCS1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationChanged Tyrosine 80 to Aspartic acidPromoterCMVAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-SOCS1-Y80F
Plasmid#129517PurposeRetroviral vector for the expression of human SOCS1 with a Y80F mutation and a FLAG tag in N-terminalDepositorInsertSOCS1 (SOCS1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationChanged Tyrosine 80 to PhenylalaninePromotercmvAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CMVs.Pl.Cre.rBG (AAV rh10)
Viral Prep#105537-rh10PurposeReady-to-use AAV rh10 (similar to AAV10) particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCMVAvailable SinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.PI.EGFP.WPRE.bGH (AAV rh10)
Viral Prep#105530-rh10PurposeReady-to-use AAV rh10 (similar to AAV10) particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCMVTagsEGFPAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+HyPer7-NLS
Plasmid#136468PurposeMammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
TagsTriple nuclear localization signal of SV40 (simia…ExpressionMammalianPromoterCMV, SP6Available SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GenEPi
Plasmid#140236PurposeMammalian expression of Piezo1-based fluorescent force sensor GenEPiDepositorInsertGenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
TagsGCaMP 6s RS1 EF4ExpressionMammalianPromotersimian CMV IE94Available SinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
ITSN-mcherry-sspB
Plasmid#85220PurposeRole of Cdc-42 selective GEF domain from ITSN in immune cell migrationDepositorInsertsTagsNAExpressionMammalianMutationR73QPromoterCMVAvailable SinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
MCS-3_Endofin_LgBiT
Plasmid#182574PurposeThis plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.DepositorInsertzinc finger FYVE-type (ZFYVE16 Human)
TagsLgBiT (Large BiT)ExpressionMammalianMutationFYVE domain Q739-K806PromoterHerpes simplex virus (HSV)Available SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-AbKRS-chIPYE
Plasmid#140020PurposePlasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integrationDepositorInsertAbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
TagsFLAGExpressionMammalianMutation1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…PromoterEF1Available SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA
Plasmid#190790Purpose[E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.DepositorInsertsCas9
mazF-LVA
UseCRISPR and Synthetic BiologyTagsLVAExpressionBacterialMutationsilent mutations of BpiI-sites in original Cas9 g…PromoterPnrsB and Ptrc-[E*](104)Available SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A
Plasmid#154773Purposeplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertG1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
TagsFLAGExpressionMammalianMutationY125A in G1PylS, hybrid PylT with A41AA and C55A …PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cry2 mCherry GRK2 ct in pcDNA3.1
Plasmid#64212PurposemCherry fused between Cry2 and GRK2ct to study cell migrationDepositorTagsmcherryExpressionMammalianPromoterCMV and cmvAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
NLS-4LNDD-pcDNA3.1(+)
Plasmid#172313PurposeExpresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cellsDepositorInsertLambda N peptide - human ADAR2 Deaminase Domain
Tags6xHis, Flag, and Nuclear Localization Signal of S…ExpressionMammalianPromoterCMVAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only