We narrowed to 166,390 results for: addgene

1. pBEG R2-dsRed-L3 #BGV203

   Plasmid

   #48973

   Purpose

   Supplies the selection marker (dsRed) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   dsRed
   

   Use

   Gateway cloning vector

   Tags

   IRES (strong)

   Promoter

   none

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pKG139

   Plasmid

   #69755

   Purpose

   Retroviral expression of CENP-C

   Depositor

   Iain Cheeseman

   Article

   Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

   Insert

   CENP-C (CENPC Human)
   

   Use

   Retroviral

   Tags

   mCherry-TEV-S

   Expression

   Mammalian

   Mutation

   L341F, N570D relative to NP_001803.2 (numbering b…

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPC-NMYC-mTRF1

   Plasmid

   #64162

   Purpose

   Retroviral vector expressing mouse TRF1 with N-terminal Myc tag

   Depositor

   Titia de Lange

   Article

   Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

   Insert

   mTRF1 (Terf1 Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

4. MAC_C_LAMP1

   Plasmid

   #111669

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   LAMP1 (LAMP1 Human)
   

   Expression

   Mammalian

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSP64T-hLbCpf1

   Plasmid

   #107187

   Purpose

   plasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)

   Depositor

   Antonio Giraldez

   Article

   Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

   Insert

   hLbCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Promoter

   sp6

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe puro Myr HA PIK3CB

   Plasmid

   #12527

   Depositor

   Jean Zhao

   Article

   Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

   Insert

   PIK3CB (PIK3CB Human)
   

   Use

   Retroviral

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Myristoylation. Constitutively active allele.

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. IFNGR2_pCSdest

   Plasmid

   #53823

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   IFNGR2 (IFNGR2 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pBABE MDER

   Plasmid

   #13494

   Depositor

   Stephen Tapscott

   Article

   Hollenberg et al Proc Natl Acad Sci U S A. 1993 Sep 1. 90

   Insert

   MyoD1-Estrogen Receptor Fusion Protein (Myod1 Mouse, Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   full length MyoD was fused to aa282-595 of Esr1. …

   Available Since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-Solo-mScarlet-H2BC11

   Plasmid

   #207756

   Purpose

   Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

    Plasmid

    #174891

    Purpose

    amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNH11 (pmyo-2::Arch(D95N)::2xMycTag)

    Plasmid

    #130275

    Purpose

    Expresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

    Insert

    Arch(D95N)
    

    Tags

    2x Myc-tag

    Expression

    Worm

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. gh39

    Plasmid

    #106713

    Purpose

    expression of gRNA targeting STT3B

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    STT3B (STT3B Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPS0263

    Plasmid

    #133228

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with PsNifH (pOGG043), gusA (pOGG083) and T-pharma (pOGG003)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPS0262

    Plasmid

    #133227

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with PsNifH (pOGG043), celB (pOGG050) and T-pharma (pOGG003)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. BPK2797

    Plasmid

    #72251

    Purpose

    Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335Q, and T1337R

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBEG R2-iBlast-L3 #BGV204

    Plasmid

    #48982

    Purpose

    Supplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Insert

    blasticidin-S deaminase
    

    Use

    Gateway cloning vector

    Tags

    IRES (weak)

    Promoter

    na

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pSCT-GAL93-SFPQ

    Plasmid

    #46320

    Depositor

    Steven Brown

    Article

    Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

    Insert

    SFPQ (SFPQ Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

18. MAC_C_EZRI

    Plasmid

    #111665

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    EZRI (EZR Human)
    

    Expression

    Mammalian

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. LLP784_SnoopCatcher-DNMT3A-CO

    Plasmid

    #211777

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Spy-EZH2
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect EZH2 e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBEG R3-miRNA(ccdB)-L4 #BGV228

    Plasmid

    #48953

    Purpose

    To create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Gateway cloning vector

    Promoter

    na

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only