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We narrowed to 301,227 results for: ins

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Showing: 7141 - 7160 of 301227 results

  1. Fyv6-NFLAG del134-173

    Plasmid
    #234135
    Purpose
    Amino acids 134-173 deleted in Fyv6 with FLAG epitope tag at N-terminus
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Expression
    Yeast
    Mutation
    C-terminus truncation
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234135-plasmid-map-sequence-id-466521

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  2. FLAG-D1-IRES-SS-D2-IRES-HA-D3

    Plasmid
    #215119
    Purpose
    Expresses FLAG-Cyclin D1, SS-Cyclin D2 and HA-Cyclin D3 in mammalian cells from an inducible lentiviral vector.
    Depositor
    Michele Pagano
    Article
    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012
    Inserts
    CCND1 (CCND1 Human)
    CCND2 (CCND2 Human)
    CCND3 (CCND3 Human)
    Use
    Lentiviral
    Tags
    FLAG-tag, HA-tag, and SS (STREP-STREP)
    Expression
    Mammalian
    Promoter
    IRES and TREG3
    Available Since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    215119-plasmid-map-sequence-id-426788

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  3. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118155
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    118155-plasmid-map-sequence-id-243704

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  4. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid
    #238007
    Purpose
    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.
    Depositor
    Simon Powell, Agnel Sfeir
    Article
    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:
    Insert
    AAVS1 (AAVS1, PPP1R12C Human)
    Use
    Rna donor to study rt-dsbr
    Expression
    Mammalian
    Mutation
    3bp insertion signature (ATC) at Cas9 cut site in…
    Promoter
    CMV
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238007-plasmid-map-sequence-id-472649

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  5. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107943-plasmid-map-sequence-id-207614

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  6. pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

    Plasmid
    #92139
    Purpose
    Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available Since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92139-plasmid-map-sequence-id-349059

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  7. pRS314-Cef1-M175I

    Plasmid
    #234118
    Purpose
    Suppressor screen mutant in Cef2
    Depositor
    Aaron Hoskins
    Insert
    Cef1
    Expression
    Yeast
    Mutation
    M175I
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234118-plasmid-map-sequence-id-466510

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  8. pRS314-Cef1-Q193P

    Plasmid
    #234117
    Purpose
    Suppressor screen mutant in Cef1
    Depositor
    Aaron Hoskins
    Insert
    Cef1
    Expression
    Yeast
    Mutation
    Q193P
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234117-plasmid-map-sequence-id-466508

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  9. pRS314-Cef1-A37V

    Plasmid
    #234119
    Purpose
    Suppressor screen mutant in Cef3
    Depositor
    Aaron Hoskins
    Insert
    Cef1
    Expression
    Yeast
    Mutation
    A37V
    Available Since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234119-plasmid-map-sequence-id-466463

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  10. pTRIPZ (M)-YFP-Eed

    Plasmid
    #82512
    Purpose
    Lentivirus vector. Expresses YFP-Eed fusion proteins in mammalian cells.
    Depositor
    Xiaojun Ren
    Insert
    embryonic ectoderm development
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    insert contains AA39-441 of EeD
    Promoter
    cmv
    Available Since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82512-plasmid-map-sequence-id-171489

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  11. pJK550

    Plasmid
    #72450
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    inserted Val2
    Promoter
    T7
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72450-plasmid-map-sequence-id-133877

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  12. mAzami Green-CytERM-N-17

    Plasmid
    #56279
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 492, Emission: 505
    Depositor
    Michael Davidson
    Insert
    CytERM
    Tags
    mAzami Green
    Expression
    Mammalian
    Mutation
    Plasmid contains first 29 AA of Rabbit P450
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    56279-plasmid-map-sequence-id-216425

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  13. pRS414-Syf1

    Plasmid
    #234114
    Purpose
    Syf1 region PCR, restriction digest and ligation into pRS414 backbone
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Expression
    Yeast
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234114-plasmid-map-sequence-id-465994

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  14. pET283xFlagPP7CPHis

    Plasmid
    #28174
    Depositor
    Kathleen Collins
    Insert
    PP7 coat protein optimized
    Tags
    6xhistidine and triple FLAG peptide
    Expression
    Bacterial
    Mutation
    C68A,C73A
    Available Since
    Feb. 24, 2011
    Availability
    Academic Institutions and Nonprofits only
    28174-plasmid-map-sequence-id-13350

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  15. CMV500 A-ATF2

    Plasmid
    #33362
    Depositor
    Charles Vinson
    Insert
    ATF
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Dominant Negative (see comments)
    Promoter
    CMV
    Available Since
    Dec. 19, 2011
    Availability
    Academic Institutions and Nonprofits only
    33362-plasmid-map-sequence-id-152190

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  16. pAc5.1C-RLuc-A90-HhR_O

    Plasmid
    #147073
    Purpose
    Insect Expression of A90-HhR
    Depositor
    Elisa Izaurralde
    Insert
    A90-HhR
    Expression
    Insect
    Mutation
    A127 instead of A90 (see comments below)
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147073-plasmid-map-sequence-id-456973

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  17. pRS416-Syf1

    Plasmid
    #234113
    Purpose
    Syf1 region PCR, restriction digest and ligation into pRS416 backbone
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Expression
    Yeast
    Available Since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234113-plasmid-map-sequence-id-466460

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  18. pCFD3.1-w-dU6:3gRNA

    Plasmid
    #123366
    Purpose
    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).
    Depositor
    Simon Bullock
    Insert
    U6:3-gRNA
    Use
    CRISPR
    Expression
    Insect
    Available Since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    123366-plasmid-map-sequence-id-242548

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  19. RABEP1A-c019

    Plasmid
    #175477
    Purpose
    Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    RABEP1 (RABEP1 Human)
    Tags
    TEV-TwinStrep
    Expression
    Insect
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175477-plasmid-map-sequence-id-353250

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  20. pHACK-GAL4>GAL80

    Plasmid
    #104874
    Purpose
    HACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.
    Depositor
    Christopher Potter
    Insert
    T2A-GAL80
    Use
    CRISPR
    Expression
    Insect
    Available Since
    March 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    104874-plasmid-map-sequence-id-204699

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Showing: 7141 - 7160 of 301227 results