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hSyn-GW-dCas9-SET2-IRES2-mCherry

Plasmid

#202089

Purpose

Neuron-specific expression vector of dCas9-Set2

Depositor

Elizabeth Heller

Article

Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

Insert

dCas9-Set2

Tags

3x FLAG and mCherry

Expression

Mammalian

Promoter

human Synapsin

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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SRSF8_RRM_pGEX

Plasmid

#135119

Purpose

Recombinant expression of SRSF8 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SRSF8 (SRSF8 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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mApple-Alpha-Actinin-19

Plasmid

#54865

Purpose

Localization: Alpha Actinin, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Stricker et al PLoS One. 2013 Jul 29;8(7):e70652. doi:

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
Hy_pMT dati

Plasmid

#91800

Purpose

Expresses the Drosophila dati circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

Insert

dati (dati Fly)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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mTFP1-H2A-10

Plasmid

#55488

Purpose

Localization: Nucleus/Histones, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

H2A (H2AC13 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGK401red-hENT1

Plasmid

#166028

Purpose

Expresses human equilibrative nucleoside transporter 1 (hENT1)

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

human equilibrative nucleoside transporter 1 (SLC29A1 Human)

Use

Synthetic Biology

Expression

Yeast

Promoter

pPGK1

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)

Plasmid

#55199

Purpose

CFP reporter along with FF4 miRNA binding sites and 28 nt sequences

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

ECFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-Craf S338A

Plasmid

#118335

Purpose

Expression of human Craf

Depositor

Philip Stork

Article

Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

Insert

Craf S338A (RAF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S338A

Promoter

CMV

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-metap2

Plasmid

#100516

Purpose

Expresse Myc tagged metap2 in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

Metap2 (METAP2 Human)

Tags

Myc

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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GCN2-KD1-pGEX4T-1

Plasmid

#21876

Depositor

David Ron

Article

Harding et al Mol Cell. 2000 Nov . 6(5):1099-108.

Insert

GCN2 (Eif2ak4 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

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NUPL2_ZNF_pGEX

Plasmid

#135135

Purpose

Recombinant expression of NUPL2 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

NUPL2 (NUPL2 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-XPLN

Plasmid

#73375

Purpose

GST-tagged expression of XPLN (ARHGEF3) in bacteria

Depositor

Jie Chen, Krister Wennerberg

Article

Arthur et al J Biol Chem. 2002 Nov 8;277(45):42964-72

Insert

arhgef3 (ARHGEF3 Human)

Tags

GST

Expression

Bacterial

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
plexm-mouse LRRC33

Plasmid

#111601

Purpose

Express mouse LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

mouse LRRC33 (Nrros Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Starts at aa 18

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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Dronpa-Mito-7

Plasmid

#57288

Purpose

Localization: Mitochondria, Excitation: 503, Emission: 518

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Insert

Mito (COX8A Human)

Tags

Dronpa

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pK RagA(Q66L)-RagC

Plasmid

#99700

Purpose

Bacterial expression of RagA(Q66L)-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

L91P

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLL3.7 shRNA against CRTC2

Plasmid

#72711

Purpose

Lentiviral expression of shRNA against CRTC2 (3rd generation)

Depositor

Freddy Jeanneteau

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

CRTC2

Use

Lentiviral

Tags

CMV-EGFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only
GAL4-mNFAT1(2-144)

Plasmid

#11108

Purpose

Expresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cells

Depositor

Anjana Rao

Article

Garcia-Rodriguez et al Eur J Immunol. 2000 Aug . 30(8):2432-6.

Insert

NFAT1 (Nfatc2 Mouse)

Tags

GAL4-DBD

Expression

Mammalian

Mutation

Contains NFAT1 amino acids 2-144; L78P and P140Q …

Available Since

April 24, 2006

Availability

Academic Institutions and Nonprofits only

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pLEX-FKBP-BclXL-Blasticidin

Plasmid

#120718

Purpose

Expresses mitochondrial membrane-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Bl2-like protein 1, isoform Bcl-X(L)

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

33 c-terminal amino acids

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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3535 pET15b Bid p22

Plasmid

#8784

Depositor

Stanley Korsmeyer

Article

Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

Insert

Bid p22 (Bid Mouse)

Tags

His

Expression

Bacterial

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pSFG-TFII-I-GFP delta

Plasmid

#22190

Depositor

Ananda Roy

Article

Desgranges et al Mol Cell Biol. 2005 Dec . 25(24):10940-5

Insert

TFII-I (GTF2I Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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