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pPB-cT3G-cERP2-MSX1

Plasmid

#222557

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of MSX1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

MSX1 (MSX1 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-PRDM9

Plasmid

#222565

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of PRDM9

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

PRDM9 (PRDM9 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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p3992 pET ubcH5

Plasmid

#8652

Depositor

Peter Howley

Article

Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

Insert

UbcH5 (UBE2D1 Human)

Expression

Bacterial

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pEPQD0KN0245

Plasmid

#162284

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPI896

Plasmid

#207453

Purpose

REMI integrated Expression for mNeonGreen-Nap1

Depositor

Rob Kay

Article

Lutton et al Curr Biol. 2023 Aug 7;33(15):3083-3096.e

Insert

Nap1

Use

Unspecified

Tags

mNeonGreen

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pEPQDKN0729

Plasmid

#162316

Purpose

TEV protease plasmid for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

T7prom-RBS-NET-TEVprotease

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJKW1658

Plasmid

#119179

Purpose

E.coli expression plasmid for Pm4CL1 and PmSPS2

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Inserts

Pm4CL1
PmSPS2

Expression

Bacterial

Promoter

pGAP

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-nGFPMNase

Plasmid

#187826

Purpose

express nGFPMNase protein in E coli

Depositor

Yikang Rong

Article

Stefanis et al Brain Res Mol Brain Res. 1998 Jan;53(1-2

Insert

nGFPMNASE

Tags

his tag

Expression

Bacterial

Promoter

T7 Promotor

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-miR-31 sponge

Plasmid

#25025

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

CMV-miR-31 sponge

Expression

Mammalian

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
pISI-mmP16 3' UTR

Plasmid

#22693

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

mmP16 3'UTR

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pFThGW

Plasmid

#27228

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus Insulin 2

Plasmid

#25786

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2002 Aug . 116(1-2):169-72.

Insert

Insulin 2

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only
pFSy(336)GW

Plasmid

#27231

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pR5-30

Plasmid

#22290

Depositor

Louise Prakash

Article

Pages et al Genetics. 2008 Sep . 180(1):73-82.

Insert

RAD5 (RAD5 Budding Yeast)

Expression

Yeast

Mutation

D681, E682->AA

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
AP-p002

Plasmid

#31493

Depositor

David Wang

Article

Gaynor et al PLoS Pathog. 2007 May 4. 3(5):e64.

Insert

Half genome of WU polyomavirus

Expression

Bacterial

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pIS1-wt Fzd3 3'UTR

Plasmid

#25024

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

wt Fzd3 3'UTR

Use

Luciferase

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only
pRGM10

Plasmid

#48790

Purpose

I-SceI DSB repair reporter cassette

Depositor

Michael Glickman

Article

Gupta et al Mol Microbiol. 2011 Jan;79(2):316-30. do

Inserts

lacZ(I-SceI)
lacZ-deltaN

Use

Dsb repair reporter

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pPtGE31_ΔPtR

Plasmid

#236260

Purpose

A version of the episome pPtGE31 with a deleted nonessential region, contains the NAT cassette

Depositor

Bogumil Karas

Article

Walker et al Nat Commun. 2026 Jan 22. doi: 10.1038/s4

Type

Empty backbone

Use

Synthetic Biology; P. tricornutum expression

Expression

Bacterial and Yeast

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCW57.1 DOX off blast POLD1

Plasmid

#241438

Purpose

Expresses DOX-suppressible POLD1

Depositor

Richard Possemato

Insert

POLD1 (POLD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Partly codon optimized

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pSLB025

Plasmid

#234634

Purpose

pET-28a(+) based plasmid for expression of H16_A1358 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A1358

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pUMN105

Plasmid

#233024

Purpose

Encodes eGFP for ORBIT integration. High copy number.

Depositor

Anthony Baughn

Article

Varshney et al bioRxiv [Preprint]. 2026 Jan 22:2026.01.

Type

Empty backbone

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-RAN

Plasmid

#222533

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of RAN

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

RAN (RAN Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-PAX5

Plasmid

#222575

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of PAX5

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

PAX5 (PAX5 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-MYBL1

Plasmid

#222547

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of MYBL1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

MYBL1 (MYBL1 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-SMAD4

Plasmid

#222573

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SMAD4

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SMAD4 (SMAD4 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ZNF541

Plasmid

#222560

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ZNF541

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

ZNF541 (ZNF541 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-NFYB

Plasmid

#222561

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of NFYB

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

NFYB (NFYB Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAN099

Plasmid

#220044

Purpose

Mammalian expression of type III-A crRNA from Streptococcus thermophilus

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

crRNA

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEPMY1CB0001

Plasmid

#162317

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven expression in E. coli. Contains mRFP1 reporter.

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0030

Plasmid

#162312

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, stop codon

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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