We narrowed to 14,176 results for: TIM
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Plasmid#60151PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB812
Plasmid#60160PurposeYeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.DepositorInsertyEVenus-PEST
TagsPEST degronExpressionYeastPromoterRNR1prAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBHM2403
Plasmid#171687PurposepRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.DepositorInsertCnoCAS9
UseCRISPRExpressionBacterial and YeastPromoterTEF1Available SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1661-sgMUC4-E3(F+E)
Plasmid#51025PurposeLentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3DepositorInsertoptimized sgRNA (MUC4 Mouse, Human)
UseCRISPR and LentiviralExpressionMammalianPromotermouse U6Available SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ heiCas9
Plasmid#186929PurposeFor in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLSDepositorInsertmyc-flexible linker-oNLS-Cas9-oNLS
UseCRISPRTagsmyc-flexible linker-optimizedNLS and optimizedNLSExpressionMammalianPromotersp6Available SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTDpelB-NTwinStrep
Plasmid#45940Purposeuse to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequenceDepositorTypeEmpty backboneTags6xHis, PelB, and Twin-StrepExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEX-CMV-SP-CFP-STM2(15-746)
Plasmid#18863DepositorInsertStromal Interaction Molecule 2 (STIM2 Human)
TagsCFPExpressionMammalianMutationCFP inserted between signal peptide and residues …Available SinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRiboI-APEX2m
Plasmid#176843PurposeExpression of APEX2 from a codon-optimized gene (single-copy integrating plasmid)DepositorInsertpRibo-APEX2m
UseIntegratingExpressionBacterialPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRibo-APEX2m
Plasmid#176842PurposeExpression of APEX2 from a codon-optimized gene (multi-copy episomal plasmid)DepositorInsertpRibo-APEX2m
ExpressionBacterialPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
JBEI-3716
Plasmid#100169PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertcodon-optimized MK
ExpressionBacterialAvailable SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNB785
Plasmid#60164PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB787
Plasmid#60152PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB789
Plasmid#60166PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB682
Plasmid#60143PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationA4VPromoterMET17prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRiboI-Sec-APEX2m
Plasmid#176845PurposeExpression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)DepositorInsertpRibo-Sec-APEX2m
ExpressionBacterialPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-opEBNA2-IRES-mCherry
Plasmid#220355PurposeExpresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression)DepositorInsertopEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
UseRetroviralExpressionMammalianMutationcodon-optimised for expression in mammalian cellsAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRibo-Sec-APEX2m
Plasmid#176844PurposeExpression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)DepositorInsertpRibo-Sec-APEX2m
UseInducibleExpressionBacterialPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLT 651
Plasmid#59998PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorExpressionBacterialMutationpartial genomicAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLT 653
Plasmid#59996PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorExpressionBacterialMutationpartial genomicAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLT 652
Plasmid#59997PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only