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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,344 results for: multi

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Showing: 701 - 720 of 18344 results

  1. pMZ374

    Plasmid
    #59896
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).
    Depositor
    Mikel Zaratiegui
    Publication
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    59896-plasmid-map-sequence-id-313658

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  3. FUW-tetO-EGFP

    Plasmid
    #84041
    Purpose
    3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoter
    Depositor
    Stefano Piccolo
    Publication
    Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    TRE-CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    84041-plasmid-map-sequence-id-490455

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  5. pMH0006

    Plasmid
    #135448
    Purpose
    Human expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.
    Depositor
    Martin Kampmann, Jonathan Weissman
    Publication
    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094
    Insert
    dCas9-BFP-KRAB
    Use
    Lentiviral
    Tags
    tagBFP
    Expression
    Mammalian
    Promoter
    EF1A
    Available since
    July 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13 Articles
    135448-plasmid-map-sequence-id-304832

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  6. pSL1777 (pEffector)

    Plasmid
    #160731
    Purpose
    Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.
    Depositor
    Samuel H. Sternberg
    Publication
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins and crRNA
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    160731-plasmid-map-sequence-id-318598

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  8. pCRISPomyces-1

    Plasmid
    #61736
    Purpose
    Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA
    Depositor
    Huimin Zhao
    Publication
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    tracrRNA
    crRNA cassette
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    61736-plasmid-map-sequence-id-256988

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  13. pDG458

    Plasmid
    #100900
    Purpose
    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    16 Articles
    100900-plasmid-map-sequence-id-457690

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  17. pCRISPathBrick

    Plasmid
    #65006
    Purpose
    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks
    Depositor
    Mattheos Koffas
    Publication
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive native promoters
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    65006-plasmid-map-sequence-id-109903

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  18. pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry

    Plasmid
    #35500
    Purpose
    AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
    Depositor
    Karl Deisseroth
    Publication
    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    CaMKIIa
    Available since
    April 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    35500-plasmid-map-sequence-id-43570

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  20. pEGFP-C1 Lifeact-EGFP-2XNLS

    Plasmid
    #58467
    Purpose
    Expresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoter
    Depositor
    Dyche Mullins
    Publication
    Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Lifeact
    2X NLS with 2X stop codons
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    58467-plasmid-map-sequence-id-516847

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Showing: 701 - 720 of 18344 results