We narrowed to 17,881 results for: puromycin
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Plasmid#158193PurposeGateway destination clone of CDK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDK2 (CDK2 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianPromoterUbiquitinAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LbdCas12a KRAB
Plasmid#188503PurposeExpresses FLAG tagged LbdCas12a KRABDepositorInsertdCas12a
UseCRISPR and LentiviralExpressionMammalianMutationD832APromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-NODAL
Plasmid#115638PurposeFor targeted integration and inducible expression of human NODAL using doxycyclineDepositorPublicationAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CAMKK2_IDG-K
Plasmid#170577PurposeGateway destination clone of CAMKK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CAMKK2 (CAMKK2 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianPromoterUbiquitinAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV LDTS-LIRmut
Plasmid#189001Purposelipophagy-inducing adaptor protein, negative controlDepositorInsertLDTS-LIRmut
UseRetroviralTagsHAAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide_GFP_NT135
Plasmid#183118PurposepLentiGuide modified to express GFP and a non-targeting sgRNADepositorInsertNT135
UseLentiviralPromoterU6Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg83-PDGFR_EF1a_iRFP670
Plasmid#186311PurposePlasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg83-PDGFR
iRFP670
TagsHis, mycExpressionMammalianAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg88-PDGFR_EF1a_eBFP2
Plasmid#186312PurposePlasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.DepositorInsertssg88-PDGFR
eBFP2
TagsHis, mycExpressionMammalianAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
px459-Hira KO sgRNA
Plasmid#186938PurposeHira KO in mouse ES cellsDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHira KO sgRNA (Hira Mouse)
UseCRISPR and Mouse TargetingExpressionMammalianPromoterU6Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2
Plasmid#183741PurposeExpresses Ki67 (8 repeats+LR)-mNeonGreenDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
TagsmNeonGreenExpressionBacterial and MammalianMutationMKI67 LR domain + 8 Ki-67 repeatsPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO.1-Tet-Puro-shIAPEz_2
Plasmid#185017PurposeshRNA mediated knockdownDepositorInsertERV_IAPEz
UseLentiviralAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-OgNLuc_SV40:PuroR
Plasmid#183038PurposeExpresses the FLAG-tagged OgNLuc (Orange LumiFluor) in mammalian cells.DepositorInsertOgNLuc LumiFluor
UseLentiviralTagsFLAGExpressionMammalianPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMX-53BP1(1-1710) 3LA
Plasmid#185698PurposeMammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.DepositorInsert53BP1(1-1710) 3LA (TP53BP1 Human)
UseRetroviralTagsFlag tag, HA tag, and tetracysteine tagExpressionMammalianMutationL175A, L517A, and L693APromoterSV40Available sinceJuly 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMX-53BP1(1-1710) 3LA7A
Plasmid#185707PurposeMammalian Expression, Retroviral vector that encodes 7A and 3LA mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of double-strand breaks and disrupts 53BP1 activity.DepositorInsert53BP1(1-1710) 3LA7A (TP53BP1 Human)
UseRetroviralTagsFlag tag, HA tag, and tetracysteine tagExpressionMammalianMutationT302A, S452A, S523A, S543A, S625A, S784A, and S89…PromoterSV40Available sinceJuly 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
plentiCRISPv2_FDX1-2
Plasmid#184489PurposeLentivirus all in one CRISPR vector targeting human FDX1 geneDepositorInsertFDX1 (FDX1 Human)
UseLentiviralAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shIAPEz_1
Plasmid#185016PurposeshRNA mediated knockdownDepositorInsertERV_IAPEz
UseLentiviralAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEH_AAV_TERT_prom_C7C_1.8kb
Plasmid#183079PurposerAAV transfer plasmid with ITRs flanking: (1) TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.DepositorInsertTERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
UseAAV; Homologous recombination donor templateMutationWildtype promoter, Exon 1 C7C (C21>T21) silent…PromoterNone for primary insert (Though partial TERT prom…Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-hPPM1A
Plasmid#185382PurposeFor mammalian expression of shRNA: AGGGTAATGGGTTGCGATATG that targets human PPM1ADepositorAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro Xlone SOX17
Plasmid#179838PurposeDoxycycline-inducible expression of human SOX17DepositorInsertSOX17 (SOX17 Human)
UseAAV, CRISPR, Synthetic Biology, and TALEN ; Donor…ExpressionMammalianPromoterTRE3GSAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 KBTBD4 P311PP
Plasmid#184625PurposeDoxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 P311PP expression in mammalian cellsDepositorInsertN-terminal Flag tagged KBTBD4 P311PP (KBTBD4 Human)
UseLentiviral; Doxycycline inducibleExpressionMammalianMutationP311PPPromoterTRE promoter, Tet ONAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only