We narrowed to 7,733 results for: CCH
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Plasmid#98745PurposeFor phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.DepositorInsertsUseSynthetic BiologyExpressionYeastMutationCodon optimized for Saccharomyces cerevisiaePromoterADH1m and PGK1Available SinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only
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PJ-INPP5E
Plasmid#38001DepositorTagsmRFPExpressionMammalianMutationCys 779 mutated to SerAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-E2-Crimson
Plasmid#117661PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-Ost1-alphaf(I)-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
PJ-DEAD
Plasmid#38002DepositorTagsmRFPExpressionMammalianMutationAsp 1263 mutated to Ala and Cys 779 mutated to SerAvailable SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pC-G418-YR
Plasmid#61767PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).DepositorInsertsnptII gene
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…PromotertrpC promoterAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPtGE34
Plasmid#107932PurposeExpresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugationDepositorInsertsOriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3
UseCRISPR and Synthetic Biology; Episomal vector for…Available SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 TIM9,10
Plasmid#170280PurposeExpresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertExpressionBacterialPromoterTim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…Available SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-dCas9-VPR
Plasmid#63801PurposepAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vectorDepositorInsertdCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionYeastPromoterGPDAvailable SinceApril 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA
Plasmid#171619PurposeAAV floxed Soma-targeted Cal-Light with KA2DepositorInsertIE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…ExpressionMammalianPromoterCMVAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
yeast_DualReporter
Plasmid#127546PurposeA dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).DepositorInsertsUseSynthetic BiologyExpressionYeastPromoterTEF, TEF2, URA3, bla (E. coli), and pTpA+MCSAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
PJ-Sac
Plasmid#38000DepositorTagsmRFPExpressionMammalianMutationAsp 1263 mutated to AlaAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-Btl2
Plasmid#117667PurposeStudy secretion efficiency of Btl2DepositorInsertpre-Ost1-alphaf(I)-Btl2
ExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)
Plasmid#198035PurposeUsed for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)DepositorAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-pML-1B_6xHis-Cas12m
Plasmid#192282PurposeMmCas12m fused to His-tag for protein purificationDepositorInsertMmCas12m
UseCRISPRTagsHis-tagExpressionBacterialPromoterT7Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-Trim46
Plasmid#176402PurposeTRIM46 tagged with GFPDepositorAvailable SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3 1 kb prom. + Enhancer CD274
Plasmid#107005PurposeModified Luciferase expression vectorDepositorInsertCD274 (CD274 Human)
UseLuciferaseExpressionMammalianMutationN.B. There are two published SNPs in this region …Available SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Trim46
Plasmid#176401PurposeTRIM46 tagged with mCherryDepositorAvailable SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final
Plasmid#107009PurposeModified Luciferase expression vectorDepositorAvailable SinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNAI-GAL4-CREB-S133A
Plasmid#46770PurposeExpression of GAL4 fusion to CREB-S133ADepositorTagsGAL4ExpressionMammalianMutationChanged CREB serine 133 to AlaninePromoterCMVAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNAI-GAL4-CREB-S142A
Plasmid#46771PurposeExpression of GAL4 fusion to CREB-S142ADepositorTagsGAL4ExpressionMammalianMutationChanged CREB serine 142 to AlaninePromoterCMVAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only