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pENTR221-NFAT5

Plasmid

#79506

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDS

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

NFAT5 (NFAT5 Human)

Use

Gateway Cloning

Promoter

no Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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f-CTS-EGFP

Plasmid

#86064

Purpose

A mammalian expression plasmid encoding fibrocystin ciliary targeting signal domain with C-terminus EGFP.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Fibrocystin (Pkhd1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

contains ciliary targeting signal (3870-3887 amin…

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pTX179

Plasmid

#89261

Purpose

Express STU (Single Transcriptional Unit) Cas9 nickase with Maize Ubiquitin1 promoter in plants

Depositor

Daniel Voytas

Publication

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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RHPMP125 (mEOS3.1-alpha Tubulin)

Plasmid

#88834

Purpose

Localization by Super-Resolution microscopy of alpha-Tubulin fused to mEOS3.1

Depositor

Ricardo Henriques

Insert

alpha-tubulin (TUBA1B) (TUBA1B Human)

Tags

mEOS3.1

Expression

Mammalian

Promoter

CMV

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-GFP-Ubl4A

Plasmid

#86921

Purpose

To express Ubl4A tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Publication

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_KDM1A

Plasmid

#86260

Purpose

Donor vector for 3' FLAG tag of human KDM1A

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Insert

KDM1A homology arms (KDM1A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCytERM_mScarlet-H_N1

Plasmid

#85067

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Tags

mScarlet-H

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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5HT2C-RNA2-Flag

Plasmid

#79679

Purpose

serotonin receptor 2C isoform 2 with C-terminal Flag tag, non-edited isoform

Depositor

Stefan Stamm

Publication

Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

Insert

serotonin receptor 2C, RNA2 (HTR2C Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-BLZinCh-1

Plasmid

#85082

Purpose

mammalian vector for the expression of a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging

Depositor

Maarten Merkx

Publication

Aper et al ACS Chem Biol. 2016 Oct 21;11(10):2854-2

Insert

BL-ZinCh-1

Expression

Mammalian

Promoter

CMV

Available since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-PH-SWAP70

Plasmid

#84582

Purpose

Expresses mCherry-tagged PH-domain of SWAP70 in mammalian cells, binds to PI(3,4)P2

Depositor

Geert van den Bogaart

Publication

Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

Insert

SWAP70 residues 209-306 (SWAP70 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Pleckstrin-homology domain, residues 209-306

Promoter

CMV

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCis GFPuv-SS Himar1 A7

Plasmid

#84686

Purpose

Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

Depositor

Ulrike Munderloh

Publication

Munderloh et al Intracellular Pathogens II: Rickettsiale

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pRSF-DUET Cas1 Cas2

Plasmid

#80088

Purpose

Encodes Cas1 and Cas2

Depositor

George Church

Publication

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 E. coli (K12))
Cas2

Expression

Bacterial

Promoter

T7

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mCerulean3

Plasmid

#75156

Purpose

Nuclear mCerulean2 (blue) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Publication

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR-TIR1

Plasmid

#83875

Purpose

Gateway donor vector for TIR1

Depositor

Jennifer Nemhauser

Publication

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

TIR1 (TIR1 Mustard Weed)

Use

Gateway Cloning

Available since

Oct. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9FL-P2A-turboGFP

Plasmid

#80941

Purpose

Plasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP.

Depositor

George Church

Publication

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9FL

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pME-TagRFPt-NS

Plasmid

#75343

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagRFPt

Depositor

Rob Parton

Publication

Don et al Zebrafish. 2016 Sep 15.

Insert

TagRFPt

Expression

Mammalian

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pEN-L1-K2-L2

Plasmid

#80684

Purpose

Gateway Entry vector containing a temperature-sensitive mouse DHFR

Depositor

Nico Dissmeyer

Publication

Faden et al Nat Commun. 2016 Jul 22;7:12202. doi: 10

Inserts

DHFR (Dhfr Mouse)
DHFR (Dhfr Mouse, Mustard Weed)

Use

Synthetic Biology

Tags

3xHA

Expression

Bacterial, Insect, Mammalia…

Mutation

mouse DHFR with N-terminal Ubiquitin

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCOLA-T7-YhjH

Plasmid

#79165

Purpose

Expresses YhjH under a T7 promoter

Depositor

Ming Hammond

Publication

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

yhjH

Expression

Bacterial

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a-Hook3 aa 1-552

Plasmid

#74614

Purpose

Expresses a truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Publication

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-552 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEB3-miRFP703

Plasmid

#79994

Purpose

EB3 (microtubule-associated protein) labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

EB3 (MAPRE3 Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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