We narrowed to 23,671 results for: crispr

1. pPPC019.AAV

   Plasmid

   #171147

   Purpose

   Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   hAAVS1 scRNA
   J1-BBa_J23117-mRFP
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119 and J1-BBa_J23117

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-Cas9C-VPR

   Plasmid

   #80933

   Purpose

   Expresses Cas9C-VPR in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9C-VPR
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pN-PITCh-3XHA

   Plasmid

   #127881

   Purpose

   Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   GFP-Puro-3XHA (PPP2CA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ230-D156R (ttLbCas12a)

   Plasmid

   #195366

   Purpose

   A temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12a

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   LbCas12a-D156R (ttLbCas12a)
   

   Use

   CRISPR; Gateway compatible lbcas12a-d156r (ttlbca…

   Expression

   Plant

   Promoter

   ZmUBI1

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

   Plasmid

   #83947

   Purpose

   gRNA cassette-carrying vector with natMX marker

   Depositor

   Irina Borodina

   Article

   Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

   Insert

   gRNA targeting ADE2 gene
   

   Expression

   Yeast

   Available Since

   Oct. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPPC005

   Plasmid

   #171140

   Purpose

   For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   XylS-Pm
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   Pm

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. miniCMV eGFP hPGK BSD

   Plasmid

   #188519

   Purpose

   Backbone for CRISPR activation (CRISPRa) isogenic PAM testing

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-NLS-dMisCas13b-SNAPf-NLS-3xFlag

   Plasmid

   #191536

   Purpose

   Overexpression of NLS-dMisCas13b-SNAPf-NLS-3xFlag in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-dMisCas13b-SNAPf-NLS-3xFlag
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-IRES-puro-NLS-dMisCas13b-EGFP-NLS

   Plasmid

   #191359

   Purpose

   Overexpression of NLS-dMisCas13b-EGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-dMisCas13b-EGFP-NLS
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. DHFR-PP7-VP64_T2A_GFP

    Plasmid

    #86167

    Purpose

    Expresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    DHFR-PP7-VP64_T2A_GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Destabilized domain (N-terminus version) of E.col…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. dSp-VP64-p65(100-261)-RTA(125-190)

    Plasmid

    #99673

    Purpose

    Expresses dSp Cas9 fused to combination of truncated activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    VP64-p65(101-261)-RTA(125-190)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. LbdCas12a KRAB

    Plasmid

    #188503

    Purpose

    Expresses FLAG tagged LbdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-dSa-VP64-p65(100-261)-RTA(125-190)

    Plasmid

    #99679

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VP64-p65(101-261)-RTA(125-190)

    Mutation

    dead Cas9

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CMV-dSa-VPR mini.-2X snRP1

    Plasmid

    #99688

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Mutation

    dead Cas9

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5

    Plasmid

    #61255

    Purpose

    CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    2xTY1-V5
    

    Use

    CRISPR

    Tags

    TY1 and V5

    Available Since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. P823_eSpCas9(1.1)-RecA

    Plasmid

    #87264

    Purpose

    Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-SUMO-AsCas12f

    Plasmid

    #171612

    Purpose

    AsCas12f1 heterologous expression plasmid

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1
    

    Expression

    Bacterial

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas34

    Plasmid

    #82386

    Purpose

    sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PEZ3

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6_(GLuc)_INT[BoBS]

    Plasmid

    #68431

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. p1071-Lenti-Single loxP

    Plasmid

    #217892

    Purpose

    Lentiviral vector, entry vector for Lentiviral Switch-OVER

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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