We narrowed to 7,745 results for: ef1a;

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pEF HaloTag-SYT1

Plasmid

#197280

Purpose

Encodes full-length wild type synaptotagmin 1 (SYT1) with an N-terminal HaloTag

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYT1 and HaloTag (Syt1 Rat)

Expression

Mammalian

Promoter

EF1alpha

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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LacZ_pHIVEGFP

Plasmid

#191251

Purpose

Expresses LacZ in mammalian cells (used as a control)

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

LacZ

Tags

EGFP

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMK1200

Plasmid

#84219

Purpose

Lentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherry

Depositor

Martin Kampmann, Jonathan Weissman

Article

Kampmann et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pHS-435

Plasmid

#195470

Purpose

Lentiviral Integratable Constitutive super IL-2 cytokine

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pEF1a-Super IL-2-2A-EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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p1.1

Plasmid

#162737

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_GFP*_Intein_DD

Plasmid

#162812

Purpose

STELLA plasmid for amber suppression of C-terminally labelled GFP

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

GFP(C-TAG)-Intein-DD

Use

Piggybac

Expression

Mammalian

Mutation

GFP(Opal247,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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aCE120

Plasmid

#204491

Purpose

Figure 1. reporter circuit

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

EBFP2
mKate2
EYFP

Use

Synthetic Biology

Tags

none

Expression

Bacterial and Mammalian

Promoter

hEF1a

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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LI A-B EF1 c2

Plasmid

#54435

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

promoter region of A. thaliana EF1alpha1 /AT1G07940.1

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_NoBody*_Intein_DD

Plasmid

#162813

Purpose

STELLA plasmid for amber suppression of C-terminally labelled NoBody

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

NoBody(C-TAG)-Intein-DD

Use

Piggybac

Expression

Mammalian

Mutation

NoBody(Opal69,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSL1045

Plasmid

#129525

Purpose

pDEF-SpCas9, Empty Vector for CRISPRi, PbEF1a promoter, 1st Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

dSpCas9 (Other)

Use

Plasmodium, rodent-infectious species

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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aCE121

Plasmid

#204492

Purpose

Cadherin circuit

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

EBFP2
Cdh6-mKate2
Cdh1-EYFP

Use

Synthetic Biology

Tags

2A

Expression

Bacterial and Mammalian

Promoter

hEF1a

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*NoBody

Plasmid

#162807

Purpose

STELLA plasmid for amber suppression of N-terminally labelled NoBody

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)NoBody

Use

Piggybac

Expression

Mammalian

Mutation

NoBody (Met1, Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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PALI1_pHIVEGFP

Plasmid

#191252

Purpose

Expresses PALI1 in mammalian cells

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

LCoR

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

NA

Promoter

EF1alpha

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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MLM3585

Plasmid

#43885

Purpose

Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: HD (binds C)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator VP64 Backbone

Use

Tale transcriptional activator expression vector

Promoter

EF1alpha

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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MLM3367

Plasmid

#43884

Purpose

Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NI (binds A)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator VP64 Backbone

Use

Tale transcriptional activator expression vector

Promoter

EF1alpha

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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MLM2579

Plasmid

#43891

Purpose

Activation Domain: NF-kB; RVD of C-terminal 0.5 TALE repeat: NG (binds T)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator p65 backbone

Use

Tale transcriptional activator expression vector

Promoter

EF1alpha

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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BII-ChPtinGW

Plasmid

#133377

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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MLM2583

Plasmid

#43889

Purpose

Activation Domain: NF-kB; RVD of C-terminal 0.5 TALE repeat: HD (binds C)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator p65 backbone

Use

Tale transcriptional activator expression vector

Promoter

EF1alpha

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
MLM3583

Plasmid

#43887

Purpose

Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NG (binds T)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator VP64 Backbone

Use

Tale transcriptional activator expression vector

Promoter

EF1alpha

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
MLM2581

Plasmid

#43888

Purpose

Activation Domain: NF-kB; RVD of C-terminal 0.5 TALE repeat: NI (binds A)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator p65 backbone

Use

Tale transcriptional activator expression vector

Promoter

EF1alpha

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge