We narrowed to 13,457 results for: sequence
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Plasmid#182321PurposePlasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertLyn-μDronpa-TagRFP-T
TagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b-LaG9(S151C)
Plasmid#172490PurposeEncodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).DepositorInsertLaG9(S151C)
Tags6xHis, PPX cleavage site, and pelB leader sequenc…ExpressionBacterialMutationS151CPromoterT7Available SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
TAL-L1-1.3-KRAB
Plasmid#103034Purpose[Edit] Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by KRAB domainDepositorInsertTAL-L1-1.3-KRAB
UseT3 in vitro transcription for expression in mamma…TagsHA and KRABAvailable SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB331
Plasmid#68659PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsEmerald Luc fused with PEST sequence [ELuc(PEST)]ExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-miR9/9*
Plasmid#177805PurposeThe pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.DepositorInsertmiR-9/9*
ExpressionMammalianAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SUMO FAAA)3
Plasmid#130703PurposeMammalian expression plasmid for negative control of mCherry-(SUMO)3.DepositorInsert3 copies of human SUMO1 separated by (GGS)4
ExpressionMammalianMutationFKVK sequence in each SUMO1 module changed to FAAAPromoterCMVAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV+ plasmid
Plasmid#169740PurposeUnregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.DepositorInserteGFP
UseSynthetic BiologyExpressionMammalianPromoterpCMV-tetO2Available SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_human-4xUAS
Plasmid#125150PurposeVector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cellsDepositorInsert4 x upstream activating sequence (UAS)
UseStap-seq screening vectorAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSA120
Plasmid#59789PurposeC. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the same operonDepositorInsertshsp-16.41 promoter
zif-1 coding
gpd-2/gpd-3 intergenic sequence
mCherry (worm)
unc-54 3' UTR
ExpressionBacterialAvailable SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP Δnef/U3
Plasmid#101346PurposeDeletion from NL4-3 nt 8911-9022 and 9088-9377. This deletes nef and U3 LTR sequencesDepositorInsertNL4-3
UseLentiviralMutationdelta nef/LTRAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
SEPTOPO
Plasmid#64944Purposesource of SEP (Super Ecliptic pHluorin) sequenceDepositorInsertSEP
ExpressionBacterialPromoterlacAvailable SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-'S-mito
Plasmid#158765PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
ExpressionMammalianMutationFlexible linkers between the protease and msGFP a…Available SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-miR124
Plasmid#177806PurposeThe pre-miR-124 sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.DepositorInsertmiR-124
ExpressionMammalianAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
TAL-L1-2.4-Ctrl
Plasmid#103037PurposePlasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning siteDepositorInsertTAL-L1-2.4
UseT3 in vitro transcription for expression in mamma…TagsFLAG and HAAvailable SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6Biso3_C
Plasmid#146088PurposeMammalian Expression of HsTNRC6Biso3DepositorInsertHsTNRC6Biso3 (TNRC6B Human)
ExpressionMammalianMutationAA 3-188 and AA residues 242-1029 (with N242D) co…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYL1300UaUf
Plasmid#183173PurposePlant binary vector with ccdB flanked by Ua and Uf unique nucleotide sequences.DepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRvACT-mCherry
Plasmid#205002PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.DepositorInsertmCherry-NLS flanked by 1kbp upstream and downstream of the actin gene from R. varieornatus
UseTardigrade expressionAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsMago_I
Plasmid#146582PurposeMammalian Expression of HsMagoDepositorInsertHsMago (MAGOH Human)
ExpressionMammalianMutationone non silent mutation Y123C compared to the seq…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
eTC GFP ß-actin ΔZip
Plasmid#27124DepositorInsertbeta-actin (ACTB Human)
TagsEGFP and TC tagExpressionMammalianMutationThis construct lacks β-actin 3′UTR and therefore …Available SinceJan. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a
Plasmid#216385PurposeBaculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)DepositorInsertNorrin (NDP Human)
UseBaculovirusTagsGP64 signal sequence-MBP-3C and Rho1D4ExpressionInsectMutationC93A/C95A/C131APromoterPolyhedrinAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only