Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 16,420 results for: GRN

Clear keywords
Showing: 7321 - 7340 of 16420 results

  1. CROPseq-Puro-4xMS2

    Plasmid
    #212626
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212626-plasmid-map-sequence-id-420274

    Enlarge

  2. pICH41331::pU6cm-pegR::EURb7

    Plasmid
    #227695
    Purpose
    Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).
    Depositor
    Jae-Yean Kim
    Article
    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41
    Insert
    Altered epegRNA backbone with cloning sites for gRNA and RTT.
    Use
    CRISPR
    Expression
    Plant
    Promoter
    U6 composite
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    227695-plasmid-map-sequence-id-452432

    Enlarge

  6. LentiGuide_DKO

    Plasmid
    #183193
    Purpose
    Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Article
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, mU6
    Available Since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183193-plasmid-map-sequence-id-360099

    Enlarge

  7. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available Since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

    Enlarge

  8. pCBC-DT2T3

    Plasmid
    #50591
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T2, gRNA scaffold, U6-29t plus, U6-1p, T3
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50591-plasmid-map-sequence-id-244476

    Enlarge

  9. pLentiGuide

    Plasmid
    #117986
    Purpose
    pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.
    Depositor
    Paul Khavari
    Article
    Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31
    Insert
    sgRNA backbone (F+E)
    Use
    Lentiviral
    Available Since
    Feb. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    117986-plasmid-map-sequence-id-235371

    Enlarge

  11. DR274

    Plasmid
    #42250
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription
    Depositor
    Keith Joung
    Article
    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR; Zebrafish expression
    Expression
    Worm
    Promoter
    T7
    Available Since
    Jan. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    42250-plasmid-map-sequence-id-254852

    Enlarge

  12. pBLU-VIPR

    Plasmid
    #220498
    Purpose
    Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).
    Depositor
    Alexander Espinosa
    Article
    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.
    Type
    Empty backbone
    Use
    CRISPR; Transposon
    Expression
    Mammalian
    Available Since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220498-plasmid-map-sequence-id-442816

    Enlarge

  14. pCBC-DT3T4

    Plasmid
    #50592
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T3, gRNA scaffold, U6-1tplus, U6-26p, T4
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50592-plasmid-map-sequence-id-372989

    Enlarge

  18. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Article
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Available Since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

    Enlarge

Showing: 7321 - 7340 of 16420 results