We narrowed to 30,814 results for: his

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pCA528 SPASTIN , residues 108-200

Plasmid

#180628

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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SUMO_MT3

Plasmid

#200303

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

Depositor

Artur Krężel

Article

Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

Insert

metallothionein 3 (MT3 Human)

Tags

Smt3

Expression

Bacterial

Promoter

T7

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CHMP1B 1-199

Plasmid

#115326

Purpose

Expresses residues 1-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-24

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Tags

His-SUMO

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pQlinkH-PTPIP51(36-235)

Plasmid

#170533

Purpose

Expresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. Coli

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Tags

His tag

Expression

Bacterial

Promoter

T5 promoter

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a Ce-iPGM-bio

Plasmid

#162559

Purpose

Expresses C. elegans iPGM containing a C-terminal biotinylation site and His tag

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

C. elegans iPGM bio-6His (ipgm-1 Nematode)

Tags

6His tag, T7 tag, Thrombin cleavage site, and bio…

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a Bm-iPGM-bio

Plasmid

#162565

Purpose

Expresses B. malayi iPGM containing a C-terminal biotinylation site and His tag

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

B. malayi iPGM bio-6His

Tags

6His tag, T7 tag, biotinylation sequence, and thr…

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-HLICNot

Plasmid

#140181

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 6x His-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

pEU3-NII-HLICNot

Promoter

T7

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2-N

Plasmid

#128308

Purpose

Express hNABP2 N-terminus (1–138 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP2 N terminal fragment (OB fold) (NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

N-terminal domain (1–138 aa)

Promoter

T7

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6

Plasmid

#127679

Purpose

Bacterial expression of His-tagged OphA-delta C6

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6

Tags

His8

Expression

Bacterial

Mutation

delta C6

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1-C

Plasmid

#128311

Purpose

Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCDB368

Plasmid

#110276

Purpose

gHH_44 with a N-terminal 10-His DsbC TEV-peptide fusion

Depositor

Christopher Bahl

Article

Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

Insert

gHH_44

Tags

10-His, Tev recognition peptide, and mature DsbC …

Expression

Bacterial

Promoter

T7

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETB-S28 histone 3 phosphorylation reporter

Plasmid

#32788

Depositor

Alice Ting

Article

Lin et al Angew Chem Int Ed Engl. 2004 May 24;43(2

Insert

CFP-14-3-3tau-H3 peptide-YFP

Tags

CFP and YFP

Expression

Bacterial

Available Since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only
Ub-LPNTG-pET

Plasmid

#221850

Purpose

Expresses Ub with sortase motif and His tag

Depositor

Artur Krężel

Article

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

Ubiquitin

Expression

Bacterial

Mutation

Arg74ProLeuThr

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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SUMO-LPQTG-pET

Plasmid

#221851

Purpose

Expresses SUMO-2 with sortase motif and His tag

Depositor

Artur Krężel

Article

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

SUMO-2 (SUMO2 Human)

Expression

Bacterial

Mutation

Gln88Leu, Gln89Pro

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAPtag5-IgK-NCAN ELS

Plasmid

#219813

Purpose

Expresses His-tagged NCAN-ELS to produce recombinant protein

Depositor

Cagla Eroglu

Article

Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

Insert

IgK (Igk Mouse)

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-degFusionRed

Plasmid

#199463

Purpose

Arabinose-induced expression of the photodegradable His-tagged protein degFusionRed in E. coli.

Depositor

Vladislav Verkhusha

Article

Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

Insert

degFusionRed

Tags

6xHis

Expression

Bacterial

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2196

Plasmid

#170400

Purpose

For expresson of LetA-[LetB PLL6->1] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ702-717, replaced with 114-130)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2197

Plasmid

#170401

Purpose

For expresson of LetA-[LetB PLL6->3] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ702-716, replace with 347-360)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2198

Plasmid

#170402

Purpose

For expresson of LetA-[LetB PLL6->5] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ702-716, replace with 580-600)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2251

Plasmid

#170403

Purpose

For expresson of LetA-[LetB PLL6->2] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ700-717, replace with 225-249)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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