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pEF-1α-p37-mClover2

Plasmid

#225500

Purpose

Expression of mClover2-tagged p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Use

Lentiviral

Tags

mClover2

Expression

Mammalian

Mutation

no

Promoter

EF1A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5a-p37WT-FLAG

Plasmid

#225498

Purpose

Expression of FLAG-tagged wildtype p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Tags

FLAG

Expression

Mammalian

Mutation

no

Promoter

CMV promoter

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5a-p37SHPmut_FLAG

Plasmid

#225499

Purpose

Expression of FLAG-tagged mutated p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Tags

FLAG

Expression

Mammalian

Mutation

F215A/E218A/QKL220-222AAA

Promoter

CMV promoter

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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p413TEF-NQYFP

Plasmid

#99556

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Du et al Mol Microbiol. 2017 Apr;104(1):105-124.

Insert

Swi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)

Tags

YFP

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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NHA-Delta 12am

Plasmid

#36935

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A Delta 12am (TNRC6A Human)

Tags

NHA

Expression

Mammalian

Mutation

aa 896-1045 only; all GW or WG changed to AA

Available Since

May 30, 2012

Availability

Academic Institutions and Nonprofits only
NHA-Delta 12b

Plasmid

#36934

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A Delta 12b (TNRC6A Human)

Tags

NHA

Expression

Mammalian

Mutation

aa 1045-1219 only

Available Since

May 30, 2012

Availability

Academic Institutions and Nonprofits only
NHA-Delta 12a

Plasmid

#36933

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A Delta 12a (TNRC6A Human)

Tags

NHA

Expression

Mammalian

Mutation

aa 896-1045 only

Available Since

May 30, 2012

Availability

Academic Institutions and Nonprofits only
Drosophila_sgRNA_group_2

Pooled Library

#134583

Purpose

Targets Drosophila orthologs of vertebrate genes that have undergone expansion in the vertebrate line

Depositor

Norbert Perrimon

Article

Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

Expression

Insect

Species

Drosophila melanogaster

Use

CRISPR

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only
pJet1.2 kanR cassette

Plasmid

#117122

Purpose

Kanamycin resistance cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Kanamycin resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-PABPC1

Plasmid

#232944

Purpose

Expresses PABPC1 protein

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

PABPC1 (PABPC1 Human)

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pNK5785

Plasmid

#219753

Purpose

pGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - nnLuz_v4 - tAOX

Use

Luciferase

Expression

Yeast

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-602_NY-ESO-1_TCR_RFP

Plasmid

#207483

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP, NY-ESO-1_TCR

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-702_CD19-BBz_CAR_tNGFR

Plasmid

#207485

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tNGFR, CD19-BBz_CAR (NGFR Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_TMPRSS2_ERG

Plasmid

#205943

Purpose

Express mEGFP-tagged fusion protein, TMPRSS2_ERG from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TMPRSS2_ERG (TMPRSS2, ERG Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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A*11:01:01:01

Plasmid

#203276

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*11:01:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pB-Tet-On-Arl13b-RhoA sensor

Plasmid

#189613

Purpose

Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

Arl13b

Tags

GFP and mScarlet

Expression

Mammalian

Promoter

Tet3G

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-GRAB-HTR6-cilia

Plasmid

#189615

Purpose

Expresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoter

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

HTR6_GRAB

Use

AAV

Promoter

Syn1

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pTR-346 pUC19-tNGFR-P2A-IL2RA N

Plasmid

#186065

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

IL2RA (IL2RA Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-345 pUC19-tNGFR-P2A-IL2RB N

Plasmid

#186064

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

IL2RB (IL2RB Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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px330-CIB1-dCas9

Plasmid

#63665

Purpose

Mammalian gRNA expression vector also expressing CIB1-dCas9

Depositor

Dong Kong

Article

Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 and CMV

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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