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pDESTmycPABPC4

Plasmid

#19877

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

PABPC4 (PABPC4 Human)

Tags

MYC

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smurf2 C716A

Plasmid

#11747

Depositor

Jeff Wrana

Article

Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

Insert

SMURF2 (SMURF2 Human)

Tags

Flag

Expression

Mammalian

Mutation

C716A: catalytically inactive

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pVTU_260_BirA

Plasmid

#63682

Purpose

Encodes E. coli biotin ligase gene (BirA) for expression in yeast cells

Depositor

Aaron Hoskins

Article

Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

Insert

BirA

Tags

6X Histidine and TEV cleavage site

Expression

Yeast

Promoter

ADH1/yeast

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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MiCy-pBAD

Plasmid

#54565

Purpose

Localization: Protein Expression Vector , Excitation: 472, Emission: 495

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Tags

6xHis and MiCy

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pFlagCMV2-UbcH13

Plasmid

#12460

Depositor

Dong-Er Zhang

Article

Zou et al Biochem Biophys Res Commun. 2005 Oct 14.

Insert

Ubc13 (UBE2N Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-S577A

Plasmid

#92235

Purpose

Express S577A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-S577A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Serine 577 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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R26TV CAG LSL 2-5

Plasmid

#68412

Purpose

Rosa26 targeting vector backbone for use with GMAP

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting; Gmap

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-D661A

Plasmid

#92236

Purpose

Express D661A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-D661A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Aspartate 661 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-H698N

Plasmid

#92242

Purpose

Express H698N mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-H698N mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Histidine 698 to Asparagine

Promoter

T7 promoter

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA-Lib

Plasmid

#53121

Purpose

sgRNA expression construction in a 3rd generation lentiviral backbone

Depositor

Wensheng Wei

Article

Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

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pUC_sgRNA

Plasmid

#68710

Purpose

Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

Depositor

Roberto Docampo

Article

Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Available Since

Sept. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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dKatushka-pBAD

Plasmid

#54775

Purpose

Localization: Protein Expression Vector , Excitation: 588, Emission: 635

Depositor

Dmitriy Chudakov, Michael Davidson

Article

Shcherbo et al Nat Methods. 2007 Sep . 4(9):741-6.

Type

Empty backbone

Tags

6xHis and dKatushka

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pZac2.1 hSynapsin1 FLEX NAPA-N SV40

Plasmid

#92283

Purpose

FRET probe; extracellular synapse targeted mCherry, Cre-dependently expressed

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

Neuron-astrocyte proximity assay - Neuronal

Use

AAV

Promoter

hSynapsin1

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-WBP2

Plasmid

#27478

Depositor

Marius Sudol

Article

Chen et al J Biol Chem. 1997 Jul 4. 272(27):17070-7

Insert

WW domain binding protein-2 (WBP2 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
pENTR4 TNRC6C

Plasmid

#20023

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Trinucleotide repeat containing 6C (TNRC6C Human)

Use

Entry vector

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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2xNLS-pMJ915v2

Plasmid

#88916

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-3xFLAG-empty-pgk-hph

Plasmid

#48754

Purpose

PiggyBac backbone, CAG promoter, Hygromycin selection, N-terminal 3xFLAG tag

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Type

Empty backbone

Use

Piggybac

Expression

Mammalian

Promoter

CAG

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only
pLenti6.2-ccdB-Renilla

Plasmid

#87073

Purpose

Lentiviral Gateway-compatible expression vector backbone with a C-terminal Renilla luciferase

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Renilla luciferase

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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mAzami Green-N1 (mAG)

Plasmid

#54798

Purpose

Localization: N1 Cloning Vector, Excitation: 492, Emission: 505

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al J Biol Chem. 2003 Sep 5. 278(36):34167-7

Type

Empty backbone

Tags

mAzami Green

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
mPapaya1-N1

Plasmid

#54732

Purpose

Localization: N1 Cloning Vector, Excitation: 530, Emission: 541

Depositor

Robert Campbell, Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Type

Empty backbone

Tags

mPapaya1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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