We narrowed to 1,058 results for: IM

1. pT7gRNA-smyhc1_977

   Plasmid

   #140867

   Purpose

   sgRNA synthesis vector for smyhc1_977 (zebrafish slow myosin heavy chain 1).

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   zebrafish smyhc1_977 sgRNA for in vitro transcription (smyhc1 Synthetic, Zebrafish)
   

   Use

   In vitro transcription of sgrnas

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2 Halo-Tev- HSPB7 (zebrafish) C117S

   Plasmid

   #108915

   Purpose

   Express C117S mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cells

   Depositor

   Yimon Aye

   Article

   Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

   Insert

   Halo-TEV-HSPB7
   

   Use

   Tags

   Halo-TEV

   Expression

   Mammalian

   Mutation

   Cysteine 117 to Serine

   Promoter

   SP6

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2 Halo-Tev- HSPB7(zebrafish) C49S

   Plasmid

   #108914

   Purpose

   Express C49S mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cells

   Depositor

   Yimon Aye

   Article

   Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

   Insert

   Halo-TEV-HSPB7
   

   Use

   Tags

   Halo-TEV

   Expression

   Mammalian

   Mutation

   Cysteine 49 to Serine

   Promoter

   SP6

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDK366

   Plasmid

   #134196

   Purpose

   Retroviral plasmid with SKAP insert

   Depositor

   Iain Cheeseman

   Article

   Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

   Insert

   5xRD SKAP Alternate Start- LAP (with G5 hardeneded) (KNSTRN Human)
   

   Use

   Retroviral

   Tags

   LAP (GFP)

   Expression

   Mammalian

   Mutation

   5xRD, Alternate Start, G5 hardeneded

   Promoter

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFR093

   Plasmid

   #134149

   Purpose

   Expression of CENP-T

   Depositor

   Iain Cheeseman

   Article

   Nishino et al EMBO J. 2013 Feb 6;32(3):424-36. doi: 10

   Insert

   CENP-T (CENPT Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   aa 1-375 with T85D mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIW040

   Plasmid

   #134127

   Purpose

   Bacterial expression of human Ska1 microtubule binding domain (MTBD)

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-Precission-human Ska1 MTBD (SKA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   aa 132-255 R236A

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIW039

   Plasmid

   #134126

   Purpose

   Bacterial expression of human Ska1 microtubule binding domain (MTBD)

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-Precission-human Ska1 MTBD (SKA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   aa 132-255 and K203A K206A

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIW028

   Plasmid

   #134125

   Purpose

   Bacterial expression of human Ska1 microtubule binding domain (MTBD)

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-Precission-human Ska1 MTBD (SKA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   aa 132-255 and K183A K184A

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. NDUFAB1 C3.3 gRNA

   Plasmid

   #90791

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NDUFAB1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   NDUFAB1 (Guide Designation C3.3) (NDUFAB1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. NDUFAB1 C4.3 gRNA

    Plasmid

    #90793

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NDUFAB1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    NDUFAB1 (Guide Designation C4.3) (NDUFAB1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. TAL3424

    Plasmid

    #47143

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-dyrk1aa-left (dyrk1aa Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCDNA3 HaloTag-V5-TurboID

    Plasmid

    #226474

    Purpose

    Expresses fusion of HaloTag and TurboID in mammalian cells

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Cell Chem Biol. 2022 Dec 15;29(12):1739-

    Insert

    TurboID
    

    Use

    Tags

    HaloTag and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pY001 (pFnCpf1_full)

    Plasmid

    #69973

    Purpose

    Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 locus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCSF107mT-GATEWAY-3'-Myc tag

    Plasmid

    #67617

    Purpose

    A pCS2 based vector to generate a 3'-Myc tag using GATEWAY cloning technology

    Depositor

    Todd Stukenberg

    Article

    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

    Type

    Empty backbone

    Use

    In vitro transcription or translation off of an s…

    Tags

    C-terminal Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SP6

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-NBRE3

    Plasmid

    #208254

    Purpose

    pGL3 promoter luciferase reporter vector containing 3 copies of the NBRE DNA response element (5′-GAGTTTTAAAAGGTCATGCTCAATT TGTC-3′) used for luciferase reporter assays in mammalian cells

    Depositor

    Douglas Kojetin

    Article

    Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

    Insert

    3X NBRE-LUC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV GFP

    Plasmid

    #49055

    Purpose

    adeno-associated virus (AAV) encoding a green fluorescent protein

    Depositor

    Fred Gage

    Article

    Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-NurRE3

    Plasmid

    #208253

    Purpose

    pGL3 promoter luciferase reporter vector containing 3 copies of the NurRE POMC promoter DNA response element (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter assays in mammalian cells

    Depositor

    Douglas Kojetin

    Article

    Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

    Insert

    3X Nur response element LUC
    

    Use

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCSF107mT-GATEWAY-3'-FLAG

    Plasmid

    #67619

    Purpose

    A pCS2 based vector to generate a 3'-Flag tag using GATEWAY cloning technology

    Depositor

    Todd Stukenberg

    Article

    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

    Type

    Empty backbone

    Use

    In vitro transcription or translation off of an s…

    Tags

    C-terminal Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SP6

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer

    Plasmid

    #90013

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-Dicer

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Ago2
    NBirA*-Dicer
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCSF107mT-GATEWAY-3'-3HA

    Plasmid

    #67616

    Purpose

    A pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technology

    Depositor

    Todd Stukenberg

    Article

    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

    Type

    Empty backbone

    Use

    In vitro transcription or translation off of an s…

    Tags

    three inframe 3' HA tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SP6

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only