We narrowed to 1,889 results for: IRES-GFP

1. pcs2-MTS-cox8A-GFP-IRES-mCherry

   Plasmid

   #226103

   Purpose

   Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   COX8A (COX8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   encodes only for mitochondrial targeting sequence…

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcs2-N-HRI(1-138)-GFP-IRES-mCherry

   Plasmid

   #226100

   Purpose

   HRI N-terminal region (residues 1-138) stability reporter construct for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   HRI (EIF2AK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains only N-HRI (aa 1-138)

   Promoter

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIRES2-eGFP:HA-mTREK-1(tandem S131C-S131C)

   Plasmid

   #224779

   Purpose

   Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Tandem TREK-1 S131C-S131C) (Kcnk2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S131C / S562C

   Promoter

   CMV

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-IRES-blast GFP-SPNS1(E164K)

   Plasmid

   #198572

   Purpose

   SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag-GFP

   Expression

   Mammalian

   Mutation

   changed glutamic acid E164 to lysine (E164K)

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-IRES-GFP/CALR ins5 YD/FL-FLAG

   Plasmid

   #214706

   Purpose

   Mammalian expression of human CALR ins5 YD/FL-FLAG

   Depositor

   Norio Komatsu

   Article

   Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

   Insert

   CALR ins5 YD/FL-FLAG (CALR Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   MSCV

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE FL NAP1 STOP C' EGFP IRES Blast

   Plasmid

   #179102

   Purpose

   Expresses untagged full length NAP1

   Depositor

   Alicia Pickrell

   Article

   Paul et al J Cell Biol. 2024 Feb 5;223(2):e20230308

   Insert

   Nak associated protein 1 (AZI2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFSynW SYT9 D197, 199N IRES GFP

   Plasmid

   #195701

   Purpose

   Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   full-length mouse SYT9 with D197, 199N mutations

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFSynW SYT9 D330, 332N IRES GFP

   Plasmid

   #195702

   Purpose

   Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   full-length mouse SYT9 with D330, 332N mutations

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-hSyn-GFP-IRES-hSNCA (A30P)

   Plasmid

   #185720

   Purpose

   AAV expression of GFP and human α-Synuclein with A30P mutation from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Changed Ala 30 to Pro

   Promoter

   hSyn

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMSCV-IRES-GFP-mouse-Caspase-1-H340D

    Plasmid

    #183362

    Purpose

    Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 H340D (Casp1 Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    H340D

    Promoter

    MESV

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMSCV-IRES-GFP-Mouse-caspase-1/11a

    Plasmid

    #183391

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    CARD domain of mouse caspase-1 fused to catalytic domain of mouse caspase-11 (Casp1 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-hSyn-GFP-IRES-hSNCA (S129D)

    Plasmid

    #185719

    Purpose

    AAV expression of GFP and human α-Synuclein with S129D mutation from hSyn promoter

    Depositor

    Jun Ding

    Article

    Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

    Insert

    synuclein, alpha (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Changed Ser 129 to Asp

    Promoter

    hSyn

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-GFP-IRES-hSNCA (S129A)

    Plasmid

    #185718

    Purpose

    AAV expression of GFP and human α-Synuclein with S129A mutation from hSyn promoter

    Depositor

    Jun Ding

    Article

    Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

    Insert

    synuclein, alpha (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Changed Ser 129 to Ala

    Promoter

    hSyn

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSCV-IRES-GFP-polar-bear-Caspase-1/4b

    Plasmid

    #183370

    Purpose

    Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1/4b
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMSCV-IRES-GFP-harbour-seal-Caspase-1/4b

    Plasmid

    #183368

    Purpose

    Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1/4b
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSCV-IRES-GFP-human-Caspase-1-H340D

    Plasmid

    #183360

    Purpose

    Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 H340D (CASP1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    H340D

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMSCV-IRES-GFP-Mouse-caspase-1/11b

    Plasmid

    #183392

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    CARD domain of mouse caspase-1 fused to mouse caspase-11 (Casp1 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

    Plasmid

    #135669

    Purpose

    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    sgPten
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-IRES-GFP-Caspase-1 (C285A) -NpLxIS

    Plasmid

    #131354

    Purpose

    Expresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its N-ternimus

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    Caspase-1 (C285A)-NpLxIS (Casp1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Caspase-1 (C285A)

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA

    Plasmid

    #121748

    Purpose

    pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporter

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    OLLAS epitope tag-IRES2-eGFP + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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