We narrowed to 1,594 results for: PTS

1. pJET-61-SFFV-T2A-PuroR-polyA-R11a

   Plasmid

   #179892

   Purpose

   Donor with homologous arms for Rab11a to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11A, member RAS oncogene family (RAB11A Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJET-22-DExCon-mNeonGreen-R11a

   Plasmid

   #179897

   Purpose

   Donor with homologous arms for Rab11a to knock in DExCon-mNeonGreen module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11A, member RAS oncogene family (RAB11A Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pESC-LEU-IpgD

   Plasmid

   #183672

   Purpose

   Inducible Shigella flexneri IpgD for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   IpgD
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-OSF-PP-squirrel-monkey-CHMP3(155)

   Plasmid

   #154183

   Purpose

   expresses squirrel monkey CHMP3(155) in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Insert

   CHMP3(155)
   

   Use

   Tags

   FLAG, Strep-Tag II, and preScission site

   Expression

   Mammalian

   Mutation

   C-terminal truncation of CHMP3 at amino acid 155

   Promoter

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. N295A (EcN), E166Q (EcN) MetNI LmH

   Plasmid

   #118269

   Purpose

   N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetI (metI Escherichia coli)
   MetN (E166Q ; N295A) (metN Escherichia coli)
   

   Use

   Tags

   6x His Tag

   Expression

   Bacterial

   Mutation

   Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…

   Promoter

   T7 Promoter

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. exon K-renilla

   Plasmid

   #81007

   Purpose

   luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

   Depositor

   Richard Baines

   Article

   Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

   Insert

   paralytic (para Fly)
   

   Use

   Tags

   Renilla luciferase

   Expression

   Insect

   Mutation

   spanning exon 24 to exon 26, termination codon in…

   Promoter

   P-AC5 (Actin 5C)

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC293 - pAAV EF1a V5-synuclein (WT)

   Plasmid

   #60057

   Purpose

   An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   alpha-synuclein (SNCA Human, human)
   

   Use

   AAV

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N1 Full length Importin beta

   Plasmid

   #106941

   Purpose

   Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

   Depositor

   Patrizia Lavia

   Article

   Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

   Insert

   human Importin beta-1 (KPNB1) (KPNB1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-GAL4-UAS-huCD25- IRES-mCherry-PGK-BFP

   Plasmid

   #223211

   Purpose

   Lentiviral vector - mCherry reporter and huCD25 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

   Depositor

   Wendell Lim

   Article

   Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

   Insert

   CD25 (IL2RA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFPC1-hVAP-A

    Plasmid

    #104447

    Purpose

    Expression of human VAP-A fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein A (VAPA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFPC1-hVAP-B

    Plasmid

    #104448

    Purpose

    Expression of human VAP-B fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein B (VAPB Human)
    

    Use

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pinducer 20 DN-KASH

    Plasmid

    #125554

    Purpose

    Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

    Depositor

    Daniel Conway

    Article

    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

    Insert

    SYNE1 (SYNE1 Human)
    

    Use

    Lentiviral

    Tags

    mcherry

    Expression

    Mutation

    Truncated form: only the KASH domain of nesprin-…

    Promoter

    tetracycline responsive element

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. anxA2-GFP

    Plasmid

    #107196

    Purpose

    Expresses human annexin A2-GFP fusion protein for live cell imaging

    Depositor

    Volker Gerke, Ursula Rescher

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    annexin A2 (ANXA2 Human)
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Ala residue 65 in human sequence replaced by Glu …

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDF-hLPYK

    Plasmid

    #220231

    Purpose

    Inducible expression of human liver pyruvate kinase

    Depositor

    Liskin Swint-Kruse, Aron Fenton

    Article

    Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

    Insert

    Human liver pyruvate kinase (PKLR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-GAL4-IL10-IRES-mCherry-PGK-BFP

    Plasmid

    #223209

    Purpose

    Lentiviral vector - mCherry reporter and IL10 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    IL10 (IL10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pD40-His/V5-c-MycT58A

    Plasmid

    #45598

    Depositor

    Rosalie Sears

    Article

    Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

    Insert

    c-Myc T58A (Myc Mouse)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    CMV

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

17. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid

    #118586

    Purpose

    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.

    Depositor

    Radhakrishnan Padmanabhan

    Article

    Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.

    Insert

    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    

    Use

    Yeast-e. col shuttle vector prs424

    Tags

    Expression

    Mutation

    The conserved GDD in the NS5 polymerase gene muta…

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-GAL4-UAS-activemTGFb1-IRES-mCherry-PGK-BFP

    Plasmid

    #223213

    Purpose

    Lentiviral vector - mCherry reporter and TGFb1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    TGFb1 active - membrane (TGFB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR-GAL4-IL35-IRES-mCherry-PGK-BFP

    Plasmid

    #223210

    Purpose

    Lentiviral vector - mCherry reporter and IL35 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    IL35 (IL12A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-GAL4-PDL1-IRES-mCherry-PGK-BFP

    Plasmid

    #223208

    Purpose

    Lentiviral vector - mCherry reporter and PDL1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    PDL1 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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