We narrowed to 10,196 results for: Uty
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Plasmid#183244PurposeExpression of human 41BBL and CD86 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cellsDepositorInsertCD86, 41BBL
ExpressionMammalianAvailable SinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNICKclos1.0
Plasmid#73639PurposeGenome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824DepositorInsertsCas9 nickase
sGRNA to pyrE
UseE.coli-clostridium shuttle vectorMutationD10APromoterj23119 and ptbAvailable SinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCASclos
Plasmid#73640PurposeTranscriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824DepositorInsertsdCas9
sgRNA to spo0A
UseE.coli-clostridium shuttle vectorMutationD10A, H840APromoterPj23119 and ptbAvailable SinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-LUC2CP/ARE
Plasmid#62857PurposeSplicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseExpressionMammalianMutationdestabilizing sequences (DS) added to the C termi…PromoterCMVAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVAX1-BMP2
Plasmid#137909PurposeConstitutive mammalian overexpression vector for human bone morphogenetic protein 2 cDNADepositorAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMIA4.721-Keppel
Plasmid#214444PurposeBxbI landing-pad HDR donor for targeting Keppel-19 GSH. CAGG-driven Casp9-P2A-Bsd.DepositorInsert2xFKBP12-Casp9
UseHdr donorTagsP2A-BsdAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIA4.721-Olônne
Plasmid#214443PurposeBxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd.DepositorInsert2xFKBP12-Casp9
UseHdr donorTagsP2A-BsdAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSH624-ssr
Plasmid#199241PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertsingle-stranded DNA recombineering
ExpressionBacterialPromoterlacIq-PtrcAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
RSV-Flag-Brd4
Plasmid#86616PurposeFlag tagged mouse Brd4 expression construct for cell transfectionDepositorAvailable SinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCCM’
Plasmid#162709PurposepCCM reconstructed after selection for CCMB1 growth on glycerol under ambient airDepositorInsertSecond CCM operon cloned from H. neapolitanus
ExpressionBacterialMutationp15A origin replaced with high-copy colE1 originPromoterPLtet0-1 promoterAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmROSA26-attP50-Neo-mKate2-3xNLS-attP50
Plasmid#183609PurposeTargeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.DepositorInsertsNeo
mKate2-3xNLS
UseMouse TargetingTags3xNLSAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAP79-Dronpa
Plasmid#182332PurposeAKAP79-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.DepositorAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCB’
Plasmid#162703PurposepCB reconstructed after selection for CCMB1 growth on glycerol under ambient airDepositorInsertpHnCB10 derived carboxysome operon, prk
ExpressionBacterialMutation5513T>G (tetR E37A); 7758G>T (second tet op…PromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
RSV-Flag-Brd2
Plasmid#86614PurposeFlag tagged mouse Brd2 expression construct for cell transfectionDepositorAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMIA4.721-Pansio
Plasmid#214441PurposeBxbI landing-pad HDR donor for targeting Pansio-1 GSH. CAGG-driven Casp9-P2A-Bsd.DepositorInsert2xFKBP12-Casp9
UseHdr donorTagsP2A-BsdAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6411
Plasmid#47050PurposeBglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coliDepositorInsertahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
UseSynthetic Biology; BglbrickExpressionBacterialPromoterPbadAvailable SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
RSV-Flag-Brd3
Plasmid#86615PurposeFlag tagged mouse Brd3 expression construct for cell transfectionDepositorAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC
Plasmid#135465PurposeMembrane-anchored TF used for yeast directed evolution of TEV proteaseDepositorInsertSTE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)-LexAVP16
TagsBFP, flagExpressionYeastPromoterTDH3Available SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 1475 (pLenti-BLAST-GATA4-P2A-H2A-mCherry)
Plasmid#233549PurposeA CMV driven human GATA4 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.DepositorInsertGATA4 (GATA4 Human)
UseLentiviralAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-MKK6-EE
Plasmid#82718PurposeExpresses constitutively active human MKK6. Multiple cloning site 2 is empty.DepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only